Addition of Riboflavin-Coupled Magnetic Beads Increases Current Production in Bioelectrochemical Systems via the Increased Formation of Anode-Biofilms
Shewanella oneidensis is one of the best-understood model organisms for extracellular electron transfer. Endogenously produced and exported flavin molecules seem to play an important role in this process and mediate the connection between respiratory enzymes on the cell surface and the insoluble sub...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00126/full |
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| author | Tutut Arinda Laura-Alina Philipp David Rehnlund Miriam Edel Jonas Chodorski Markus Stöckl Dirk Holtmann Roland Ulber Johannes Gescher Johannes Gescher Katrin Sturm-Richter |
| author_facet | Tutut Arinda Laura-Alina Philipp David Rehnlund Miriam Edel Jonas Chodorski Markus Stöckl Dirk Holtmann Roland Ulber Johannes Gescher Johannes Gescher Katrin Sturm-Richter |
| author_sort | Tutut Arinda |
| collection | DOAJ |
| description | Shewanella oneidensis is one of the best-understood model organisms for extracellular electron transfer. Endogenously produced and exported flavin molecules seem to play an important role in this process and mediate the connection between respiratory enzymes on the cell surface and the insoluble substrate by acting as electron shuttle and cytochrome-bound cofactor. Consequently, the addition of riboflavin to a bioelectrochemical system (BES) containing S. oneidensis cells as biocatalyst leads to a strong current increase. Still, an external application of riboflavin to increase current production in continuously operating BESs does not seem to be applicable due to the constant washout of the soluble flavin compound. In this study, we developed a recyclable electron shuttle to overcome the limitation of mediator addition to BES. Riboflavin was coupled to magnetic beads that can easily be recycled from the medium. The effect on current production and cell distribution in a BES as well as the recovery rate and the stability of the beads was investigated. The addition of synthesized beads leads to a more than twofold higher current production, which was likely caused by increased biofilm production. Moreover, 90% of the flavin-coupled beads could be recovered from the BESs using a magnetic separator. |
| first_indexed | 2024-12-18T11:56:40Z |
| format | Article |
| id | doaj.art-9f18fb119fd04399a38fcdb993bf9216 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-18T11:56:40Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-9f18fb119fd04399a38fcdb993bf92162022-12-21T21:09:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-02-011010.3389/fmicb.2019.00126437979Addition of Riboflavin-Coupled Magnetic Beads Increases Current Production in Bioelectrochemical Systems via the Increased Formation of Anode-BiofilmsTutut Arinda0Laura-Alina Philipp1David Rehnlund2Miriam Edel3Jonas Chodorski4Markus Stöckl5Dirk Holtmann6Roland Ulber7Johannes Gescher8Johannes Gescher9Katrin Sturm-Richter10Institute for Applied Biosciences, Department of Applied Biology, Karlsruhe Institute of Technology, Karlsruhe, GermanyInstitute for Applied Biosciences, Department of Applied Biology, Karlsruhe Institute of Technology, Karlsruhe, GermanyInstitute for Applied Biosciences, Department of Applied Biology, Karlsruhe Institute of Technology, Karlsruhe, GermanyInstitute for Applied Biosciences, Department of Applied Biology, Karlsruhe Institute of Technology, Karlsruhe, GermanyChair of Bioprocess Engineering, Technical University of Kaiserslautern, Kaiserslautern, GermanyElectrochemistry, DECHEMA-Forschungsinstitut, Frankfurt, GermanyIndustrial Biotechnology, DECHEMA-Forschungsinstitut, Frankfurt, GermanyChair of Bioprocess Engineering, Technical University of Kaiserslautern, Kaiserslautern, GermanyInstitute for Applied Biosciences, Department of Applied Biology, Karlsruhe Institute of Technology, Karlsruhe, GermanyInstitute for Biological Interfaces, Karlsruhe Institute of Technology, Karlsruhe, GermanyInstitute for Applied Biosciences, Department of Applied Biology, Karlsruhe Institute of Technology, Karlsruhe, GermanyShewanella oneidensis is one of the best-understood model organisms for extracellular electron transfer. Endogenously produced and exported flavin molecules seem to play an important role in this process and mediate the connection between respiratory enzymes on the cell surface and the insoluble substrate by acting as electron shuttle and cytochrome-bound cofactor. Consequently, the addition of riboflavin to a bioelectrochemical system (BES) containing S. oneidensis cells as biocatalyst leads to a strong current increase. Still, an external application of riboflavin to increase current production in continuously operating BESs does not seem to be applicable due to the constant washout of the soluble flavin compound. In this study, we developed a recyclable electron shuttle to overcome the limitation of mediator addition to BES. Riboflavin was coupled to magnetic beads that can easily be recycled from the medium. The effect on current production and cell distribution in a BES as well as the recovery rate and the stability of the beads was investigated. The addition of synthesized beads leads to a more than twofold higher current production, which was likely caused by increased biofilm production. Moreover, 90% of the flavin-coupled beads could be recovered from the BESs using a magnetic separator.https://www.frontiersin.org/article/10.3389/fmicb.2019.00126/fullShewanella oneidensisbioelectrochemical systemselectron shuttleflavinsmagnetic beadsextracellular electron transfer |
| spellingShingle | Tutut Arinda Laura-Alina Philipp David Rehnlund Miriam Edel Jonas Chodorski Markus Stöckl Dirk Holtmann Roland Ulber Johannes Gescher Johannes Gescher Katrin Sturm-Richter Addition of Riboflavin-Coupled Magnetic Beads Increases Current Production in Bioelectrochemical Systems via the Increased Formation of Anode-Biofilms Frontiers in Microbiology Shewanella oneidensis bioelectrochemical systems electron shuttle flavins magnetic beads extracellular electron transfer |
| title | Addition of Riboflavin-Coupled Magnetic Beads Increases Current Production in Bioelectrochemical Systems via the Increased Formation of Anode-Biofilms |
| title_full | Addition of Riboflavin-Coupled Magnetic Beads Increases Current Production in Bioelectrochemical Systems via the Increased Formation of Anode-Biofilms |
| title_fullStr | Addition of Riboflavin-Coupled Magnetic Beads Increases Current Production in Bioelectrochemical Systems via the Increased Formation of Anode-Biofilms |
| title_full_unstemmed | Addition of Riboflavin-Coupled Magnetic Beads Increases Current Production in Bioelectrochemical Systems via the Increased Formation of Anode-Biofilms |
| title_short | Addition of Riboflavin-Coupled Magnetic Beads Increases Current Production in Bioelectrochemical Systems via the Increased Formation of Anode-Biofilms |
| title_sort | addition of riboflavin coupled magnetic beads increases current production in bioelectrochemical systems via the increased formation of anode biofilms |
| topic | Shewanella oneidensis bioelectrochemical systems electron shuttle flavins magnetic beads extracellular electron transfer |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00126/full |
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