Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk
The flunixin residues in foods of animal origin could pose hazards for human health. In this paper, a broad-selectivity polyclonal antibody was obtained using 5-hydroxyflunixin coupled with bovine serum albumin as the immunogen. The semi-inhibitory concentration (IC50) of the polyclonal antibody was...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2019.1577365 |
| _version_ | 1818126581472690176 |
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| author | Xiaona Chen Shimin Peng Chen Liu Xin Zou Yuebin Ke Wenxiao Jiang |
| author_facet | Xiaona Chen Shimin Peng Chen Liu Xin Zou Yuebin Ke Wenxiao Jiang |
| author_sort | Xiaona Chen |
| collection | DOAJ |
| description | The flunixin residues in foods of animal origin could pose hazards for human health. In this paper, a broad-selectivity polyclonal antibody was obtained using 5-hydroxyflunixin coupled with bovine serum albumin as the immunogen. The semi-inhibitory concentration (IC50) of the polyclonal antibody was 1.43 ng/mL for flunixin and 0.29 ng/mL for 5-hydroxyflunixin, respectively, which were far below the maximum residue limits set by the United States, the European Union and China. The limits of detection were calculated to be 2.98 μg/kg for flunixin in bovine muscle and 0.78 μg/L for 5-hydroxyflunixin in milk. In spike and recovery tests, the average recovery ranged from 83% to 105%, with a satisfying coefficient of variance ranging from 5.8% to 11.3%. Furthermore, the ELISA method was validated by a well-established LC-MS/MS method. These results proved that the established method is suitable for flunixin and 5-hydroxyflunixin residue analysis. |
| first_indexed | 2024-12-11T07:03:44Z |
| format | Article |
| id | doaj.art-9f1a660a2929498c8c47a0ba85e6c035 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-11T07:03:44Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-9f1a660a2929498c8c47a0ba85e6c0352022-12-22T01:16:34ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432019-01-0130132033210.1080/09540105.2019.15773651577365Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milkXiaona Chen0Shimin Peng1Chen Liu2Xin Zou3Yuebin Ke4Wenxiao Jiang5Shenzhen University Health Sciences CenterShenzhen University Health Sciences CenterShenzhen People’s HospitalShenzhen University Health Sciences CenterShenzhen Center for Disease Control and PreventionShenzhen University Health Sciences CenterThe flunixin residues in foods of animal origin could pose hazards for human health. In this paper, a broad-selectivity polyclonal antibody was obtained using 5-hydroxyflunixin coupled with bovine serum albumin as the immunogen. The semi-inhibitory concentration (IC50) of the polyclonal antibody was 1.43 ng/mL for flunixin and 0.29 ng/mL for 5-hydroxyflunixin, respectively, which were far below the maximum residue limits set by the United States, the European Union and China. The limits of detection were calculated to be 2.98 μg/kg for flunixin in bovine muscle and 0.78 μg/L for 5-hydroxyflunixin in milk. In spike and recovery tests, the average recovery ranged from 83% to 105%, with a satisfying coefficient of variance ranging from 5.8% to 11.3%. Furthermore, the ELISA method was validated by a well-established LC-MS/MS method. These results proved that the established method is suitable for flunixin and 5-hydroxyflunixin residue analysis.http://dx.doi.org/10.1080/09540105.2019.1577365flunixin5-hydroxyflunixinimmunoassaybovine musclemilk |
| spellingShingle | Xiaona Chen Shimin Peng Chen Liu Xin Zou Yuebin Ke Wenxiao Jiang Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk Food and Agricultural Immunology flunixin 5-hydroxyflunixin immunoassay bovine muscle milk |
| title | Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk |
| title_full | Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk |
| title_fullStr | Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk |
| title_full_unstemmed | Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk |
| title_short | Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk |
| title_sort | development of an indirect competitive enzyme linked immunosorbent assay for detecting flunixin and 5 hydroxyflunixin residues in bovine muscle and milk |
| topic | flunixin 5-hydroxyflunixin immunoassay bovine muscle milk |
| url | http://dx.doi.org/10.1080/09540105.2019.1577365 |
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