ICSI using fresh and frozen PESA-TESA spermatozoa to examine assisted reproductive outcome retrospectively
ObjectiveThe male reproductive system generates, accumulates, and transports the sperm. In this study, 2 methods of surgically retrieving sperm, namely, testicular sperm aspiration (TESA) and percutaneous epididymal sperm aspiration (PESA), are discussed and studied in men aged ≤38 years to achieve...
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| Format: | Article |
| Language: | English |
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Korean Society of Obstetrics and Gynecology
2019-11-01
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| Series: | Obstetrics & Gynecology Science |
| Subjects: | |
| Online Access: | http://ogscience.org/upload/pdf/ogs-62-429.pdf |
| _version_ | 1818414735633154048 |
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| author | Aamir Javed Manjula Kannasandra Ramaiah Muralidhar Srinivasaih Talkad |
| author_facet | Aamir Javed Manjula Kannasandra Ramaiah Muralidhar Srinivasaih Talkad |
| author_sort | Aamir Javed |
| collection | DOAJ |
| description | ObjectiveThe male reproductive system generates, accumulates, and transports the sperm. In this study, 2 methods of surgically retrieving sperm, namely, testicular sperm aspiration (TESA) and percutaneous epididymal sperm aspiration (PESA), are discussed and studied in men aged ≤38 years to achieve successful conception using assisted reproductive technology. The purpose was to assess the fertilization rate (FA), clinical pregnancy, and live birth rate (LBR) with sperm.MethodsA total of 287 semen samples were divided into 4 groups as follows: fresh PESA (n=73), frozen PESA (n=65), fresh TESA (n=128), and frozen TESA (n=21). The DNA fragmentation test using sperm chromatin dispersion assay was measured and reported.ResultsFA was 70.3% and 65.5%, (P<0.022) for fresh and frozen epididymal sperm and 53.8% and 49.5%, (P<0.032) for fresh and frozen testicular sperm. LBR was 33.6% and 30.2% (P<0.075) for fresh and frozen epididymal sperm (PESA) and 22.7% and 18.2% (P<0.063) for fresh and frozen-thawed TESA sperm.ConclusionExposure to tissue shearing may adversely affect sperm quality. Increased sperm DNA damage due to long-term exposure while teasing enhances reactive oxygen species production foremost to membrane damage because of the oxidation of polyunsaturated fatty acid in lipids (lipid peroxidation), oxidation of amino acid in proteins, and inactivation of specific enzymes, all leading to enzymatic dipping and possibility of less fertilization and conception as indicated by the increase in LBR with fresh/frozen PESA compared to with fresh/frozen TESA. |
| first_indexed | 2024-12-14T11:23:49Z |
| format | Article |
| id | doaj.art-9f1bda5486ca41ddbd9897c002fd70e8 |
| institution | Directory Open Access Journal |
| issn | 2287-8572 2287-8580 |
| language | English |
| last_indexed | 2024-12-14T11:23:49Z |
| publishDate | 2019-11-01 |
| publisher | Korean Society of Obstetrics and Gynecology |
| record_format | Article |
| series | Obstetrics & Gynecology Science |
| spelling | doaj.art-9f1bda5486ca41ddbd9897c002fd70e82022-12-21T23:03:38ZengKorean Society of Obstetrics and GynecologyObstetrics & Gynecology Science2287-85722287-85802019-11-0162642943710.5468/ogs.2019.62.6.429609ICSI using fresh and frozen PESA-TESA spermatozoa to examine assisted reproductive outcome retrospectivelyAamir Javed0Manjula Kannasandra Ramaiah1Muralidhar Srinivasaih Talkad2Department of Biotechnology, REVA University, Bangalore, .IndiaDepartment of Biotechnology, REVA University, Bangalore, .IndiaPost Graduate-Department of Biotechnology, Dayananda Sagar Institutions, Bangalore, .IndiaObjectiveThe male reproductive system generates, accumulates, and transports the sperm. In this study, 2 methods of surgically retrieving sperm, namely, testicular sperm aspiration (TESA) and percutaneous epididymal sperm aspiration (PESA), are discussed and studied in men aged ≤38 years to achieve successful conception using assisted reproductive technology. The purpose was to assess the fertilization rate (FA), clinical pregnancy, and live birth rate (LBR) with sperm.MethodsA total of 287 semen samples were divided into 4 groups as follows: fresh PESA (n=73), frozen PESA (n=65), fresh TESA (n=128), and frozen TESA (n=21). The DNA fragmentation test using sperm chromatin dispersion assay was measured and reported.ResultsFA was 70.3% and 65.5%, (P<0.022) for fresh and frozen epididymal sperm and 53.8% and 49.5%, (P<0.032) for fresh and frozen testicular sperm. LBR was 33.6% and 30.2% (P<0.075) for fresh and frozen epididymal sperm (PESA) and 22.7% and 18.2% (P<0.063) for fresh and frozen-thawed TESA sperm.ConclusionExposure to tissue shearing may adversely affect sperm quality. Increased sperm DNA damage due to long-term exposure while teasing enhances reactive oxygen species production foremost to membrane damage because of the oxidation of polyunsaturated fatty acid in lipids (lipid peroxidation), oxidation of amino acid in proteins, and inactivation of specific enzymes, all leading to enzymatic dipping and possibility of less fertilization and conception as indicated by the increase in LBR with fresh/frozen PESA compared to with fresh/frozen TESA.http://ogscience.org/upload/pdf/ogs-62-429.pdfspermatozoafertilizationinfertilitydna fragmentation |
| spellingShingle | Aamir Javed Manjula Kannasandra Ramaiah Muralidhar Srinivasaih Talkad ICSI using fresh and frozen PESA-TESA spermatozoa to examine assisted reproductive outcome retrospectively Obstetrics & Gynecology Science spermatozoa fertilization infertility dna fragmentation |
| title | ICSI using fresh and frozen PESA-TESA spermatozoa to examine assisted reproductive outcome retrospectively |
| title_full | ICSI using fresh and frozen PESA-TESA spermatozoa to examine assisted reproductive outcome retrospectively |
| title_fullStr | ICSI using fresh and frozen PESA-TESA spermatozoa to examine assisted reproductive outcome retrospectively |
| title_full_unstemmed | ICSI using fresh and frozen PESA-TESA spermatozoa to examine assisted reproductive outcome retrospectively |
| title_short | ICSI using fresh and frozen PESA-TESA spermatozoa to examine assisted reproductive outcome retrospectively |
| title_sort | icsi using fresh and frozen pesa tesa spermatozoa to examine assisted reproductive outcome retrospectively |
| topic | spermatozoa fertilization infertility dna fragmentation |
| url | http://ogscience.org/upload/pdf/ogs-62-429.pdf |
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