Functional imaging of conduction dynamics in cortical and spinal axons
Mammalian axons are specialized for transmitting action potentials to targets within the central and peripheral nervous system. A growing body of evidence suggests that, besides signal conduction, axons play essential roles in neural information processing, and their malfunctions are common hallmark...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2023-08-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/86512 |
| _version_ | 1797739335630454784 |
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| author | Milos Radivojevic Anna Rostedt Punga |
| author_facet | Milos Radivojevic Anna Rostedt Punga |
| author_sort | Milos Radivojevic |
| collection | DOAJ |
| description | Mammalian axons are specialized for transmitting action potentials to targets within the central and peripheral nervous system. A growing body of evidence suggests that, besides signal conduction, axons play essential roles in neural information processing, and their malfunctions are common hallmarks of neurodegenerative diseases. The technologies available to study axonal function and structure integrally limit the comprehension of axon neurobiology. High-density microelectrode arrays (HD-MEAs) allow for accessing axonal action potentials at high spatiotemporal resolution, but provide no insights on axonal morphology. Here, we demonstrate a method for electrical visualization of axonal morphologies based on extracellular action potentials recorded from cortical and motor neurons using HD-MEAs. The method enabled us to reconstruct up to 5-cm-long axonal arbors and directly monitor axonal conduction across thousands of recording sites. We reconstructed 1.86 m of cortical and spinal axons in total and found specific features in their structure and function. |
| first_indexed | 2024-03-12T13:56:05Z |
| format | Article |
| id | doaj.art-9f1cb544923941f5a51416ac90d83bc8 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-03-12T13:56:05Z |
| publishDate | 2023-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-9f1cb544923941f5a51416ac90d83bc82023-08-22T14:33:12ZengeLife Sciences Publications LtdeLife2050-084X2023-08-011210.7554/eLife.86512Functional imaging of conduction dynamics in cortical and spinal axonsMilos Radivojevic0https://orcid.org/0000-0001-8828-9475Anna Rostedt Punga1Uppsala University, Department of Medical Sciences, Uppsala, SwedenUppsala University, Department of Medical Sciences, Uppsala, SwedenMammalian axons are specialized for transmitting action potentials to targets within the central and peripheral nervous system. A growing body of evidence suggests that, besides signal conduction, axons play essential roles in neural information processing, and their malfunctions are common hallmarks of neurodegenerative diseases. The technologies available to study axonal function and structure integrally limit the comprehension of axon neurobiology. High-density microelectrode arrays (HD-MEAs) allow for accessing axonal action potentials at high spatiotemporal resolution, but provide no insights on axonal morphology. Here, we demonstrate a method for electrical visualization of axonal morphologies based on extracellular action potentials recorded from cortical and motor neurons using HD-MEAs. The method enabled us to reconstruct up to 5-cm-long axonal arbors and directly monitor axonal conduction across thousands of recording sites. We reconstructed 1.86 m of cortical and spinal axons in total and found specific features in their structure and function.https://elifesciences.org/articles/86512high-density microelectrode arrays (HD-MEAs)extracellular action potentialsaxonal conductionbioassaycortical neuronsmotor neurons |
| spellingShingle | Milos Radivojevic Anna Rostedt Punga Functional imaging of conduction dynamics in cortical and spinal axons eLife high-density microelectrode arrays (HD-MEAs) extracellular action potentials axonal conduction bioassay cortical neurons motor neurons |
| title | Functional imaging of conduction dynamics in cortical and spinal axons |
| title_full | Functional imaging of conduction dynamics in cortical and spinal axons |
| title_fullStr | Functional imaging of conduction dynamics in cortical and spinal axons |
| title_full_unstemmed | Functional imaging of conduction dynamics in cortical and spinal axons |
| title_short | Functional imaging of conduction dynamics in cortical and spinal axons |
| title_sort | functional imaging of conduction dynamics in cortical and spinal axons |
| topic | high-density microelectrode arrays (HD-MEAs) extracellular action potentials axonal conduction bioassay cortical neurons motor neurons |
| url | https://elifesciences.org/articles/86512 |
| work_keys_str_mv | AT milosradivojevic functionalimagingofconductiondynamicsincorticalandspinalaxons AT annarostedtpunga functionalimagingofconductiondynamicsincorticalandspinalaxons |