Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoa
Background: Sperm DNA integrity is considered an important parameter to assess seminal fluid quality and can be used as a predictive test of potential fertility. Amongst the various tests to determine sperm DNA integrity, one is the Acridine Orange test. Recent studies have demonstrated the importan...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | Journal of Human Reproductive Sciences |
| Subjects: | |
| Online Access: | http://www.jhrsonline.org/article.asp?issn=0974-1208;year=2014;volume=7;issue=3;spage=212;epage=217;aulast=Raimondo |
| _version_ | 1811261569075511296 |
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| author | Salvatore Raimondo Tommaso Gentile Felice Cuomo Stefania De Filippo Gilda E Aprea John Guida |
| author_facet | Salvatore Raimondo Tommaso Gentile Felice Cuomo Stefania De Filippo Gilda E Aprea John Guida |
| author_sort | Salvatore Raimondo |
| collection | DOAJ |
| description | Background: Sperm DNA integrity is considered an important parameter to assess seminal fluid quality and can be used as a predictive test of potential fertility. Amongst the various tests to determine sperm DNA integrity, one is the Acridine Orange test. Recent studies have demonstrated the importance of p53 in maintaining sperm DNA integrity.
The aim of this study was to assess if a p53 ELISA assay could be a new indicator of DNA damage in human spermatozoa. Materials and Methods: 103 human semen samples were evaluated using both Acridine Orange test and p53 ELISA and results were compared. Results: A clear correlation between the values measured by two methods was obtained. Conclusions: If this hypothesis will be confirmed by further studies, the p53 ELISA assay could become a new and more precise indicator of DNA damage in human spermatozoa. |
| first_indexed | 2024-04-12T19:06:47Z |
| format | Article |
| id | doaj.art-9f1d1880f013439083ba5c8c35d134ad |
| institution | Directory Open Access Journal |
| issn | 0974-1208 1998-4766 |
| language | English |
| last_indexed | 2024-04-12T19:06:47Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Human Reproductive Sciences |
| spelling | doaj.art-9f1d1880f013439083ba5c8c35d134ad2022-12-22T03:20:00ZengWolters Kluwer Medknow PublicationsJournal of Human Reproductive Sciences0974-12081998-47662014-01-017321221710.4103/0974-1208.142490Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoaSalvatore RaimondoTommaso GentileFelice CuomoStefania De FilippoGilda E ApreaJohn GuidaBackground: Sperm DNA integrity is considered an important parameter to assess seminal fluid quality and can be used as a predictive test of potential fertility. Amongst the various tests to determine sperm DNA integrity, one is the Acridine Orange test. Recent studies have demonstrated the importance of p53 in maintaining sperm DNA integrity. The aim of this study was to assess if a p53 ELISA assay could be a new indicator of DNA damage in human spermatozoa. Materials and Methods: 103 human semen samples were evaluated using both Acridine Orange test and p53 ELISA and results were compared. Results: A clear correlation between the values measured by two methods was obtained. Conclusions: If this hypothesis will be confirmed by further studies, the p53 ELISA assay could become a new and more precise indicator of DNA damage in human spermatozoa.http://www.jhrsonline.org/article.asp?issn=0974-1208;year=2014;volume=7;issue=3;spage=212;epage=217;aulast=RaimondoAcridine Orange testP53sperm DNA damagesperm DNA fragmentation index |
| spellingShingle | Salvatore Raimondo Tommaso Gentile Felice Cuomo Stefania De Filippo Gilda E Aprea John Guida Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoa Journal of Human Reproductive Sciences Acridine Orange test P53 sperm DNA damage sperm DNA fragmentation index |
| title | Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoa |
| title_full | Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoa |
| title_fullStr | Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoa |
| title_full_unstemmed | Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoa |
| title_short | Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoa |
| title_sort | quantitative evaluation of p53 as a new indicator of dna damage in human spermatozoa |
| topic | Acridine Orange test P53 sperm DNA damage sperm DNA fragmentation index |
| url | http://www.jhrsonline.org/article.asp?issn=0974-1208;year=2014;volume=7;issue=3;spage=212;epage=217;aulast=Raimondo |
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