Application of Single Extracellular Vesicle Analysis Techniques
Junquan Zhu, Feifeng Wu,* Cuifang Li,* Jueyi Mao,* Yang Wang,* Xin Zhou,* Haotian Xie,* Chuan Wen Department of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, People’s Republic of China*These authors contributed eq...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-09-01
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| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/application-of-single-extracellular-vesicle-analysis-techniques-peer-reviewed-fulltext-article-IJN |
| _version_ | 1797678105800736768 |
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| author | Zhu J Wu F Li C Mao J Wang Y Zhou X Xie H Wen C |
| author_facet | Zhu J Wu F Li C Mao J Wang Y Zhou X Xie H Wen C |
| author_sort | Zhu J |
| collection | DOAJ |
| description | Junquan Zhu, Feifeng Wu,* Cuifang Li,* Jueyi Mao,* Yang Wang,* Xin Zhou,* Haotian Xie,* Chuan Wen Department of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chuan Wen, Department of Pediatrics, The Second Xiangya Hospital, Central South University, 139 Renmin Middle Road, Changsha, Hunan, 410011, People’s Republic of China, Tel +86-13755191815, Email chuanwen@csu.edu.cnAbstract: Extracellular vesicles (EVs) are lipid containers that are actively released by cells and contain complex molecular cargoes. These cargoes include abundant material such as genomes and proteins from cells of origin. They are involved in intercellular communication and various pathological processes, showing excellent potential for diagnosing and treating diseases. Given the significant heterogeneity of EVs in complex physiopathological processes, unveiling their composition is essential to understanding their function. Bulk detection methods have been previously used to analyze EVs, but they often mask their heterogeneity, leading to the loss of valuable information. To overcome this limitation, single extracellular vesicle (SEV) analysis techniques have been developed and advanced. These techniques allow for analyzing EVs’ physical information and biometric molecules at the SEV level. This paper reviews recent advances in SEV detection methods and summarizes some clinical applications for SEV detection strategies.Keywords: single extracellular vesicle analysis, heterogeneity, detection methods, disease diagnosis, new therapies |
| first_indexed | 2024-03-11T22:54:51Z |
| format | Article |
| id | doaj.art-9f2240286eb4464b9c6694ec8e5475bf |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-03-11T22:54:51Z |
| publishDate | 2023-09-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-9f2240286eb4464b9c6694ec8e5475bf2023-09-21T19:08:58ZengDove Medical PressInternational Journal of Nanomedicine1178-20132023-09-01Volume 185365537686821Application of Single Extracellular Vesicle Analysis TechniquesZhu JWu FLi CMao JWang YZhou XXie HWen CJunquan Zhu, Feifeng Wu,* Cuifang Li,* Jueyi Mao,* Yang Wang,* Xin Zhou,* Haotian Xie,* Chuan Wen Department of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chuan Wen, Department of Pediatrics, The Second Xiangya Hospital, Central South University, 139 Renmin Middle Road, Changsha, Hunan, 410011, People’s Republic of China, Tel +86-13755191815, Email chuanwen@csu.edu.cnAbstract: Extracellular vesicles (EVs) are lipid containers that are actively released by cells and contain complex molecular cargoes. These cargoes include abundant material such as genomes and proteins from cells of origin. They are involved in intercellular communication and various pathological processes, showing excellent potential for diagnosing and treating diseases. Given the significant heterogeneity of EVs in complex physiopathological processes, unveiling their composition is essential to understanding their function. Bulk detection methods have been previously used to analyze EVs, but they often mask their heterogeneity, leading to the loss of valuable information. To overcome this limitation, single extracellular vesicle (SEV) analysis techniques have been developed and advanced. These techniques allow for analyzing EVs’ physical information and biometric molecules at the SEV level. This paper reviews recent advances in SEV detection methods and summarizes some clinical applications for SEV detection strategies.Keywords: single extracellular vesicle analysis, heterogeneity, detection methods, disease diagnosis, new therapieshttps://www.dovepress.com/application-of-single-extracellular-vesicle-analysis-techniques-peer-reviewed-fulltext-article-IJNsingle extracellular vesicle analysisheterogeneitydetection methodsdisease diagnosisnew therapies |
| spellingShingle | Zhu J Wu F Li C Mao J Wang Y Zhou X Xie H Wen C Application of Single Extracellular Vesicle Analysis Techniques International Journal of Nanomedicine single extracellular vesicle analysis heterogeneity detection methods disease diagnosis new therapies |
| title | Application of Single Extracellular Vesicle Analysis Techniques |
| title_full | Application of Single Extracellular Vesicle Analysis Techniques |
| title_fullStr | Application of Single Extracellular Vesicle Analysis Techniques |
| title_full_unstemmed | Application of Single Extracellular Vesicle Analysis Techniques |
| title_short | Application of Single Extracellular Vesicle Analysis Techniques |
| title_sort | application of single extracellular vesicle analysis techniques |
| topic | single extracellular vesicle analysis heterogeneity detection methods disease diagnosis new therapies |
| url | https://www.dovepress.com/application-of-single-extracellular-vesicle-analysis-techniques-peer-reviewed-fulltext-article-IJN |
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