Acetylation of carboxyl-terminal domain selectively regulates p53-mediated gene transcriptionn
Objective To explore whether carboxyl-terminal domain (CTD) acetylation contributes to selectively regulating p53-mediated transcription of a subset of genes. Methods Based on p53-null lung cancer cell line H1299, a pair of inducible cell strains expressing the wildtype p53 (p53-WT) and the...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-11-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210799.pdf |
| _version_ | 1827390537374629888 |
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| author | YAN Xiao-jun, XU Wen-bin, WANG Dong-lai |
| author_facet | YAN Xiao-jun, XU Wen-bin, WANG Dong-lai |
| author_sort | YAN Xiao-jun, XU Wen-bin, WANG Dong-lai |
| collection | DOAJ |
| description | Objective To explore whether carboxyl-terminal domain (CTD) acetylation contributes to selectively regulating p53-mediated transcription of a subset of genes. Methods Based on p53-null lung cancer cell line H1299, a pair of inducible cell strains expressing the wildtype p53 (p53-WT) and the CTD acetylation-mimicking p53 (p53-6KQ) were constructal, respectively, and a response to doxycycline (Doxy) treatment was generated. RNA-sequencing was applied to analyze the changes of the transcriptional profiles regulated by p53-WT or p53-6KQ. Bio-informatic analysis was performed to annotate p53-6KQ-specific candidates, and their potential biological functions. Results The p53-inducible cell strains were successfully established. 306 differentially expressed genes that were specifically regulated by p53-6KQ were identified. These genes were functionally enriched in the biological processes involving in cell fate determination, transcription, neuron development and tumor necrosis factor signaling pathway. Conclusions The CTD acetylation may selectively and functionally regulate a subset of p53 target genes. |
| first_indexed | 2024-03-08T16:54:06Z |
| format | Article |
| id | doaj.art-9f228aa038c34cc49cc013e4fd3ad442 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:54:06Z |
| publishDate | 2021-11-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-9f228aa038c34cc49cc013e4fd3ad4422024-01-05T03:07:24ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252021-11-01411115771582Acetylation of carboxyl-terminal domain selectively regulates p53-mediated gene transcriptionnYAN Xiao-jun, XU Wen-bin, WANG Dong-lai0Department of Medical Genetics, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005, ChinaObjective To explore whether carboxyl-terminal domain (CTD) acetylation contributes to selectively regulating p53-mediated transcription of a subset of genes. Methods Based on p53-null lung cancer cell line H1299, a pair of inducible cell strains expressing the wildtype p53 (p53-WT) and the CTD acetylation-mimicking p53 (p53-6KQ) were constructal, respectively, and a response to doxycycline (Doxy) treatment was generated. RNA-sequencing was applied to analyze the changes of the transcriptional profiles regulated by p53-WT or p53-6KQ. Bio-informatic analysis was performed to annotate p53-6KQ-specific candidates, and their potential biological functions. Results The p53-inducible cell strains were successfully established. 306 differentially expressed genes that were specifically regulated by p53-6KQ were identified. These genes were functionally enriched in the biological processes involving in cell fate determination, transcription, neuron development and tumor necrosis factor signaling pathway. Conclusions The CTD acetylation may selectively and functionally regulate a subset of p53 target genes.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210799.pdfp53|acetylation|gene transcriptional regulation|tumor|bioinformatic analysis |
| spellingShingle | YAN Xiao-jun, XU Wen-bin, WANG Dong-lai Acetylation of carboxyl-terminal domain selectively regulates p53-mediated gene transcriptionn Jichu yixue yu linchuang p53|acetylation|gene transcriptional regulation|tumor|bioinformatic analysis |
| title | Acetylation of carboxyl-terminal domain selectively regulates p53-mediated gene transcriptionn |
| title_full | Acetylation of carboxyl-terminal domain selectively regulates p53-mediated gene transcriptionn |
| title_fullStr | Acetylation of carboxyl-terminal domain selectively regulates p53-mediated gene transcriptionn |
| title_full_unstemmed | Acetylation of carboxyl-terminal domain selectively regulates p53-mediated gene transcriptionn |
| title_short | Acetylation of carboxyl-terminal domain selectively regulates p53-mediated gene transcriptionn |
| title_sort | acetylation of carboxyl terminal domain selectively regulates p53 mediated gene transcriptionn |
| topic | p53|acetylation|gene transcriptional regulation|tumor|bioinformatic analysis |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210799.pdf |
| work_keys_str_mv | AT yanxiaojunxuwenbinwangdonglai acetylationofcarboxylterminaldomainselectivelyregulatesp53mediatedgenetranscriptionn |