Metabolomics approach: Interpretation of changes in rat plasma metabolites after solifenacin treatment
Abstract Changes in metabolite levels of patients using the long-term drug can be comprehensively demonstrated by pharmacometabolomic studies. In this study, biological alterations induced by the administration of solifenacin succinate were investigated with a pharmacometabolomics approach on rat me...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2022-11-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100755&tlng=en |
| _version_ | 1811215447614291968 |
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| author | Tugrul Cagri Akman Yucel Kadioglu Onur Senol Beyzagul Erkayman |
| author_facet | Tugrul Cagri Akman Yucel Kadioglu Onur Senol Beyzagul Erkayman |
| author_sort | Tugrul Cagri Akman |
| collection | DOAJ |
| description | Abstract Changes in metabolite levels of patients using the long-term drug can be comprehensively demonstrated by pharmacometabolomic studies. In this study, biological alterations induced by the administration of solifenacin succinate were investigated with a pharmacometabolomics approach on rat metabolism. Plasma samples obtained from rats were analyzed by LC-Q- TOF/MS/MS. METLIN and HMDB databases were used to identify metabolites. Data were processed and classified with MATLAB 2017b. 53 m/z values were found to be significantly different between the drug and control groups (p ≤ 0.01 and fold analysis > 1.5) and identified by comparing METLIN and HMDB databases. According to multivariate data analysis, changes in arachidonic acid, thromboxane A2, palmitic acid, choline, calcitriol, histamine phosphate, retinyl ester, l-cysteine, l-leucine, beta-alanine, l-histidine levels were found to be statistically significant compare to the control group. Differences in the biosynthesis of phenylalanine, aminoacyl-tRNA, tyrosine, tryptophan, metabolism of glycerophospholipid, cysteine, methionine, histidine, arachidonic metabolism have been successfully demonstrated by the metabolomics approach. Our study provides important information to explain the efficacy and toxicity of chronic administration of solifenacin succinate. |
| first_indexed | 2024-04-12T06:22:48Z |
| format | Article |
| id | doaj.art-9f25113cd0394e1c8d41d6883ffa8561 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-12T06:22:48Z |
| publishDate | 2022-11-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-9f25113cd0394e1c8d41d6883ffa85612022-12-22T03:44:16ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902022-11-015810.1590/s2175-97902022e20849Metabolomics approach: Interpretation of changes in rat plasma metabolites after solifenacin treatmentTugrul Cagri Akmanhttps://orcid.org/0000-0002-5439-950XYucel KadiogluOnur SenolBeyzagul ErkaymanAbstract Changes in metabolite levels of patients using the long-term drug can be comprehensively demonstrated by pharmacometabolomic studies. In this study, biological alterations induced by the administration of solifenacin succinate were investigated with a pharmacometabolomics approach on rat metabolism. Plasma samples obtained from rats were analyzed by LC-Q- TOF/MS/MS. METLIN and HMDB databases were used to identify metabolites. Data were processed and classified with MATLAB 2017b. 53 m/z values were found to be significantly different between the drug and control groups (p ≤ 0.01 and fold analysis > 1.5) and identified by comparing METLIN and HMDB databases. According to multivariate data analysis, changes in arachidonic acid, thromboxane A2, palmitic acid, choline, calcitriol, histamine phosphate, retinyl ester, l-cysteine, l-leucine, beta-alanine, l-histidine levels were found to be statistically significant compare to the control group. Differences in the biosynthesis of phenylalanine, aminoacyl-tRNA, tyrosine, tryptophan, metabolism of glycerophospholipid, cysteine, methionine, histidine, arachidonic metabolism have been successfully demonstrated by the metabolomics approach. Our study provides important information to explain the efficacy and toxicity of chronic administration of solifenacin succinate.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100755&tlng=enSolifenacin succinateMetabolomicsRatLC-Q-TOF/MS/MS |
| spellingShingle | Tugrul Cagri Akman Yucel Kadioglu Onur Senol Beyzagul Erkayman Metabolomics approach: Interpretation of changes in rat plasma metabolites after solifenacin treatment Brazilian Journal of Pharmaceutical Sciences Solifenacin succinate Metabolomics Rat LC-Q-TOF/MS/MS |
| title | Metabolomics approach: Interpretation of changes in rat plasma metabolites after solifenacin treatment |
| title_full | Metabolomics approach: Interpretation of changes in rat plasma metabolites after solifenacin treatment |
| title_fullStr | Metabolomics approach: Interpretation of changes in rat plasma metabolites after solifenacin treatment |
| title_full_unstemmed | Metabolomics approach: Interpretation of changes in rat plasma metabolites after solifenacin treatment |
| title_short | Metabolomics approach: Interpretation of changes in rat plasma metabolites after solifenacin treatment |
| title_sort | metabolomics approach interpretation of changes in rat plasma metabolites after solifenacin treatment |
| topic | Solifenacin succinate Metabolomics Rat LC-Q-TOF/MS/MS |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100755&tlng=en |
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