The dynamics of protein localisation to restricted zones within Drosophila mechanosensory cilia
Abstract The Drosophila chordotonal neuron cilium is the site of mechanosensory transduction. The cilium has a 9 + 0 axoneme structure and is highly sub-compartmentalised, with proximal and distal zones harbouring different TRP channels and the proximal zone axoneme also being decorated with axonema...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-17189-w |
| _version_ | 1811315427797630976 |
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| author | Wangchu Xiang Petra zur Lage Fay G. Newton Guiyun Qiu Andrew P. Jarman |
| author_facet | Wangchu Xiang Petra zur Lage Fay G. Newton Guiyun Qiu Andrew P. Jarman |
| author_sort | Wangchu Xiang |
| collection | DOAJ |
| description | Abstract The Drosophila chordotonal neuron cilium is the site of mechanosensory transduction. The cilium has a 9 + 0 axoneme structure and is highly sub-compartmentalised, with proximal and distal zones harbouring different TRP channels and the proximal zone axoneme also being decorated with axonemal dynein motor complexes. The activity of the dynein complexes is essential for mechanotransduction. We investigate the localisation of TRP channels and dynein motor complexes during ciliogenesis. Differences in timing of TRP channel localisation correlate with order of construction of the two ciliary zones. Dynein motor complexes are initially not confined to their target proximal zone, but ectopic complexes beyond the proximal zone are later cleared, perhaps by retrograde transport. Differences in transient distal localisation of outer and inner dynein arm complexes (ODAs and IDAs) are consistent with previous suggestions from unicellular eukaryotes of differences in processivity during intraflagellar transport. Stable localisation depends on the targeting of their docking proteins in the proximal zone. For ODA, we characterise an ODA docking complex (ODA-DC) that is targeted directly to the proximal zone. Interestingly, the subunit composition of the ODA-DC in chordotonal neuron cilia appears to be different from the predicted ODA-DC in Drosophila sperm. |
| first_indexed | 2024-04-13T11:29:54Z |
| format | Article |
| id | doaj.art-9f25ccdf2c954285af1577de29332309 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T11:29:54Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9f25ccdf2c954285af1577de293323092022-12-22T02:48:35ZengNature PortfolioScientific Reports2045-23222022-08-0112111610.1038/s41598-022-17189-wThe dynamics of protein localisation to restricted zones within Drosophila mechanosensory ciliaWangchu Xiang0Petra zur Lage1Fay G. Newton2Guiyun Qiu3Andrew P. Jarman4Centre for Discovery Brain Sciences, Edinburgh Medical School, University of EdinburghCentre for Discovery Brain Sciences, Edinburgh Medical School, University of EdinburghCentre for Discovery Brain Sciences, Edinburgh Medical School, University of EdinburghCentre for Discovery Brain Sciences, Edinburgh Medical School, University of EdinburghCentre for Discovery Brain Sciences, Edinburgh Medical School, University of EdinburghAbstract The Drosophila chordotonal neuron cilium is the site of mechanosensory transduction. The cilium has a 9 + 0 axoneme structure and is highly sub-compartmentalised, with proximal and distal zones harbouring different TRP channels and the proximal zone axoneme also being decorated with axonemal dynein motor complexes. The activity of the dynein complexes is essential for mechanotransduction. We investigate the localisation of TRP channels and dynein motor complexes during ciliogenesis. Differences in timing of TRP channel localisation correlate with order of construction of the two ciliary zones. Dynein motor complexes are initially not confined to their target proximal zone, but ectopic complexes beyond the proximal zone are later cleared, perhaps by retrograde transport. Differences in transient distal localisation of outer and inner dynein arm complexes (ODAs and IDAs) are consistent with previous suggestions from unicellular eukaryotes of differences in processivity during intraflagellar transport. Stable localisation depends on the targeting of their docking proteins in the proximal zone. For ODA, we characterise an ODA docking complex (ODA-DC) that is targeted directly to the proximal zone. Interestingly, the subunit composition of the ODA-DC in chordotonal neuron cilia appears to be different from the predicted ODA-DC in Drosophila sperm.https://doi.org/10.1038/s41598-022-17189-w |
| spellingShingle | Wangchu Xiang Petra zur Lage Fay G. Newton Guiyun Qiu Andrew P. Jarman The dynamics of protein localisation to restricted zones within Drosophila mechanosensory cilia Scientific Reports |
| title | The dynamics of protein localisation to restricted zones within Drosophila mechanosensory cilia |
| title_full | The dynamics of protein localisation to restricted zones within Drosophila mechanosensory cilia |
| title_fullStr | The dynamics of protein localisation to restricted zones within Drosophila mechanosensory cilia |
| title_full_unstemmed | The dynamics of protein localisation to restricted zones within Drosophila mechanosensory cilia |
| title_short | The dynamics of protein localisation to restricted zones within Drosophila mechanosensory cilia |
| title_sort | dynamics of protein localisation to restricted zones within drosophila mechanosensory cilia |
| url | https://doi.org/10.1038/s41598-022-17189-w |
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