| Summary: | In recent decades, the development of non-destructive measurement methods for agricultural commodities has gained a lot of attention among scientists, but these techniques have different levels of accuracy for each instrument used. Therefore, this study aimed to compare the prediction accuracy of natural pigments, such as Total Carotenoid Content (TCC) and Total Flavonoid Content (TFC) using a color spectrophotometer and Visible/Near-Infrared (Vis/NIR) spectroscopy (381–1065 nm). The effect of ethephon concentration on the spectral characteristics and the accuracy of predicting pigments was studied. The samples used include cucumber fruit, which consisted of the 'Mars', 'Vanesa', and 'Roberto’ varieties. During the planting of the fruit, ethephon was applied at different concentrations of 0 ppm, 150 ppm, and 300 ppm. The results showed that the best accuracy for predicting TCC was obtained through a color spectrophotometer (Rcal = 0.89, Rpred = 0.90, RPD = 2.44), while the best prediction for TFC was the Vis/NIR spectroscopy (Rcal = 0.86, Rpred = 0.83, RPD = 1.78). Furthermore, the ethephon affects the spectral characteristics of cucumber fruit. Ethephon concentration of 150 ppm produced the highest accuracy value compared to others. This study proved that the use of non-destructive measurement methods with a color spectrophotometer and Vis/NIR spectroscopy has good performance in predicting TCC and TFC. The techniques are also easy to use, do not require chemicals, and have high accuracy.
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