Comparison of color spectrophotometer and Vis/NIR spectroscopy on assessing natural pigments of cucumber applied with different ethephon concentrations

In recent decades, the development of non-destructive measurement methods for agricultural commodities has gained a lot of attention among scientists, but these techniques have different levels of accuracy for each instrument used. Therefore, this study aimed to compare the prediction accuracy of na...

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Main Authors: Kusumiyati Kusumiyati, Ine Elisa Putri
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:Heliyon
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844023097724
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author Kusumiyati Kusumiyati
Ine Elisa Putri
author_facet Kusumiyati Kusumiyati
Ine Elisa Putri
author_sort Kusumiyati Kusumiyati
collection DOAJ
description In recent decades, the development of non-destructive measurement methods for agricultural commodities has gained a lot of attention among scientists, but these techniques have different levels of accuracy for each instrument used. Therefore, this study aimed to compare the prediction accuracy of natural pigments, such as Total Carotenoid Content (TCC) and Total Flavonoid Content (TFC) using a color spectrophotometer and Visible/Near-Infrared (Vis/NIR) spectroscopy (381–1065 nm). The effect of ethephon concentration on the spectral characteristics and the accuracy of predicting pigments was studied. The samples used include cucumber fruit, which consisted of the 'Mars', 'Vanesa', and 'Roberto’ varieties. During the planting of the fruit, ethephon was applied at different concentrations of 0 ppm, 150 ppm, and 300 ppm. The results showed that the best accuracy for predicting TCC was obtained through a color spectrophotometer (Rcal = 0.89, Rpred = 0.90, RPD = 2.44), while the best prediction for TFC was the Vis/NIR spectroscopy (Rcal = 0.86, Rpred = 0.83, RPD = 1.78). Furthermore, the ethephon affects the spectral characteristics of cucumber fruit. Ethephon concentration of 150 ppm produced the highest accuracy value compared to others. This study proved that the use of non-destructive measurement methods with a color spectrophotometer and Vis/NIR spectroscopy has good performance in predicting TCC and TFC. The techniques are also easy to use, do not require chemicals, and have high accuracy.
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spelling doaj.art-9f28b32d32e341e4b2a4973f563799972023-12-21T07:33:49ZengElsevierHeliyon2405-84402023-12-01912e22564Comparison of color spectrophotometer and Vis/NIR spectroscopy on assessing natural pigments of cucumber applied with different ethephon concentrationsKusumiyati Kusumiyati0Ine Elisa Putri1Master Program of Agronomy, Faculty of Agriculture, Universitas Padjadjaran, Sumedang 45363, Indonesia; Laboratory of Horticulture, Faculty of Agriculture, Universitas Padjadjaran, Sumedang 45363, Indonesia; Department of Agronomy, Faculty of Agriculture, Universitas Padjadjaran, Sumedang 45363, Indonesia; Corresponding author. Master Program of Agronomy, Faculty of Agriculture, Universitas Padjadjaran, Sumedang 45363, Indonesia.Master Program of Agronomy, Faculty of Agriculture, Universitas Padjadjaran, Sumedang 45363, Indonesia; Laboratory of Horticulture, Faculty of Agriculture, Universitas Padjadjaran, Sumedang 45363, IndonesiaIn recent decades, the development of non-destructive measurement methods for agricultural commodities has gained a lot of attention among scientists, but these techniques have different levels of accuracy for each instrument used. Therefore, this study aimed to compare the prediction accuracy of natural pigments, such as Total Carotenoid Content (TCC) and Total Flavonoid Content (TFC) using a color spectrophotometer and Visible/Near-Infrared (Vis/NIR) spectroscopy (381–1065 nm). The effect of ethephon concentration on the spectral characteristics and the accuracy of predicting pigments was studied. The samples used include cucumber fruit, which consisted of the 'Mars', 'Vanesa', and 'Roberto’ varieties. During the planting of the fruit, ethephon was applied at different concentrations of 0 ppm, 150 ppm, and 300 ppm. The results showed that the best accuracy for predicting TCC was obtained through a color spectrophotometer (Rcal = 0.89, Rpred = 0.90, RPD = 2.44), while the best prediction for TFC was the Vis/NIR spectroscopy (Rcal = 0.86, Rpred = 0.83, RPD = 1.78). Furthermore, the ethephon affects the spectral characteristics of cucumber fruit. Ethephon concentration of 150 ppm produced the highest accuracy value compared to others. This study proved that the use of non-destructive measurement methods with a color spectrophotometer and Vis/NIR spectroscopy has good performance in predicting TCC and TFC. The techniques are also easy to use, do not require chemicals, and have high accuracy.http://www.sciencedirect.com/science/article/pii/S2405844023097724Food scanningFruit qualityNon-destructivePrediction
spellingShingle Kusumiyati Kusumiyati
Ine Elisa Putri
Comparison of color spectrophotometer and Vis/NIR spectroscopy on assessing natural pigments of cucumber applied with different ethephon concentrations
Heliyon
Food scanning
Fruit quality
Non-destructive
Prediction
title Comparison of color spectrophotometer and Vis/NIR spectroscopy on assessing natural pigments of cucumber applied with different ethephon concentrations
title_full Comparison of color spectrophotometer and Vis/NIR spectroscopy on assessing natural pigments of cucumber applied with different ethephon concentrations
title_fullStr Comparison of color spectrophotometer and Vis/NIR spectroscopy on assessing natural pigments of cucumber applied with different ethephon concentrations
title_full_unstemmed Comparison of color spectrophotometer and Vis/NIR spectroscopy on assessing natural pigments of cucumber applied with different ethephon concentrations
title_short Comparison of color spectrophotometer and Vis/NIR spectroscopy on assessing natural pigments of cucumber applied with different ethephon concentrations
title_sort comparison of color spectrophotometer and vis nir spectroscopy on assessing natural pigments of cucumber applied with different ethephon concentrations
topic Food scanning
Fruit quality
Non-destructive
Prediction
url http://www.sciencedirect.com/science/article/pii/S2405844023097724
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AT ineelisaputri comparisonofcolorspectrophotometerandvisnirspectroscopyonassessingnaturalpigmentsofcucumberappliedwithdifferentethephonconcentrations