Assessment of Bacterial Communities Within the Biofilm of Bladder Calculi in the Neurogenic Bladder Rat Model Following Spinal Cord Injury
Purpose To develop a rat model of bladder calculi in the neurogenic bladder following spinal cord injury (SCI) and assess bacterial communities within the biofilm of bladder calculi using denaturing gradient gel electrophoresis (DGGE). Methods The silk tied to a small segment of the Teflon IV cathet...
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| Format: | Article |
| Language: | English |
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Korean Continence Society
2022-03-01
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| Series: | International Neurourology Journal |
| Subjects: | |
| Online Access: | http://www.einj.org/upload/pdf/inj-2142182-091.pdf |
| _version_ | 1811311678486216704 |
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| author | Jeong Woo Lee Sang-Seob Lee Seung Ho Yang Hyun-Sop Choe |
| author_facet | Jeong Woo Lee Sang-Seob Lee Seung Ho Yang Hyun-Sop Choe |
| author_sort | Jeong Woo Lee |
| collection | DOAJ |
| description | Purpose To develop a rat model of bladder calculi in the neurogenic bladder following spinal cord injury (SCI) and assess bacterial communities within the biofilm of bladder calculi using denaturing gradient gel electrophoresis (DGGE). Methods The silk tied to a small segment of the Teflon IV catheter was implanted through the urethra into the bladder of rats with SCI induced by T9 laminectomy. After 6 months, the rats were sacrificed and their bladder calculi were collected by opening the bladders through the low-midline incision. Genomic DNA was extracted from the biofilm of bladder calculi followed by DGGE to obtain bacterial DNA. The DNA sequences were compared and analyzed using BLAST (Basic Local Alignment Search Tool) to identify bacteria. Results After placing silk nidus in the bladder for 6 months, all 6 rats developed bladder calculi. According to DGGE analysis, Pseudomonas aeruginosa was the most dominant strain, while Clostridium sp. and Lactobacillus sp. were relatively dominant strains within the biofilm of bladder calculi in the rats with SCI. Conclusions DGGE analysis showed various microorganisms in the biofilm of calculi arising from a neurogenic bladder rat model. This research design can be the basis for clinical studies and may be applied to calculi in patients with neurogenic bladder following SCI. |
| first_indexed | 2024-04-13T10:23:09Z |
| format | Article |
| id | doaj.art-9f2a5159caf04e43881ea75952a86e93 |
| institution | Directory Open Access Journal |
| issn | 2093-4777 2093-6931 |
| language | English |
| last_indexed | 2024-04-13T10:23:09Z |
| publishDate | 2022-03-01 |
| publisher | Korean Continence Society |
| record_format | Article |
| series | International Neurourology Journal |
| spelling | doaj.art-9f2a5159caf04e43881ea75952a86e932022-12-22T02:50:26ZengKorean Continence SocietyInternational Neurourology Journal2093-47772093-69312022-03-01261263010.5213/inj.2142182.091988Assessment of Bacterial Communities Within the Biofilm of Bladder Calculi in the Neurogenic Bladder Rat Model Following Spinal Cord InjuryJeong Woo Lee0Sang-Seob Lee1Seung Ho Yang2Hyun-Sop Choe3 Department of Urology, Kyung Hee University Medical Center, Kyung Hee University College of Medicine, Seoul, Korea Department of Life Science, Graduate School, Kyonggi University, Suwon, Korea Department of Neurosurgery, St. Vincent’s Hospital, Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea, Seoul, Korea Department of Urology, St. Vincent’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, KoreaPurpose To develop a rat model of bladder calculi in the neurogenic bladder following spinal cord injury (SCI) and assess bacterial communities within the biofilm of bladder calculi using denaturing gradient gel electrophoresis (DGGE). Methods The silk tied to a small segment of the Teflon IV catheter was implanted through the urethra into the bladder of rats with SCI induced by T9 laminectomy. After 6 months, the rats were sacrificed and their bladder calculi were collected by opening the bladders through the low-midline incision. Genomic DNA was extracted from the biofilm of bladder calculi followed by DGGE to obtain bacterial DNA. The DNA sequences were compared and analyzed using BLAST (Basic Local Alignment Search Tool) to identify bacteria. Results After placing silk nidus in the bladder for 6 months, all 6 rats developed bladder calculi. According to DGGE analysis, Pseudomonas aeruginosa was the most dominant strain, while Clostridium sp. and Lactobacillus sp. were relatively dominant strains within the biofilm of bladder calculi in the rats with SCI. Conclusions DGGE analysis showed various microorganisms in the biofilm of calculi arising from a neurogenic bladder rat model. This research design can be the basis for clinical studies and may be applied to calculi in patients with neurogenic bladder following SCI.http://www.einj.org/upload/pdf/inj-2142182-091.pdfbladder calculineurogenic bladderbiofilmdenaturing gradient gel electrophoresisrat model |
| spellingShingle | Jeong Woo Lee Sang-Seob Lee Seung Ho Yang Hyun-Sop Choe Assessment of Bacterial Communities Within the Biofilm of Bladder Calculi in the Neurogenic Bladder Rat Model Following Spinal Cord Injury International Neurourology Journal bladder calculi neurogenic bladder biofilm denaturing gradient gel electrophoresis rat model |
| title | Assessment of Bacterial Communities Within the Biofilm of Bladder Calculi in the Neurogenic Bladder Rat Model Following Spinal Cord Injury |
| title_full | Assessment of Bacterial Communities Within the Biofilm of Bladder Calculi in the Neurogenic Bladder Rat Model Following Spinal Cord Injury |
| title_fullStr | Assessment of Bacterial Communities Within the Biofilm of Bladder Calculi in the Neurogenic Bladder Rat Model Following Spinal Cord Injury |
| title_full_unstemmed | Assessment of Bacterial Communities Within the Biofilm of Bladder Calculi in the Neurogenic Bladder Rat Model Following Spinal Cord Injury |
| title_short | Assessment of Bacterial Communities Within the Biofilm of Bladder Calculi in the Neurogenic Bladder Rat Model Following Spinal Cord Injury |
| title_sort | assessment of bacterial communities within the biofilm of bladder calculi in the neurogenic bladder rat model following spinal cord injury |
| topic | bladder calculi neurogenic bladder biofilm denaturing gradient gel electrophoresis rat model |
| url | http://www.einj.org/upload/pdf/inj-2142182-091.pdf |
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