OPTIMATION OF PLATELET RICH PLASMA PRODUCTION PROTOCOL USING DOUBLE CENTRIFUGATION METHODS

Currently, therapy with Platelet Rich Plasma (PRP) has been widely used and continues to grow for various clinical applications. Along with its development, there are various options in the method of obtaining PRP, either automatic or manual, while one of the most reliable according to the literatur...

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Main Authors: Hans Kristian Nugraha, Yetti Hernaningsih, Jusak Nugraha
Format: Article
Language:English
Published: Universitas Airlangga 2016-11-01
Series:Folia Medica Indonesiana
Subjects:
Online Access:https://e-journal.unair.ac.id/FMI/article/view/2832
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author Hans Kristian Nugraha
Yetti Hernaningsih
Jusak Nugraha
author_facet Hans Kristian Nugraha
Yetti Hernaningsih
Jusak Nugraha
author_sort Hans Kristian Nugraha
collection DOAJ
description Currently, therapy with Platelet Rich Plasma (PRP) has been widely used and continues to grow for various clinical applications. Along with its development, there are various options in the method of obtaining PRP, either automatic or manual, while one of the most reliable according to the literature is a double centrifugation method. The purpose of this research is to produce an optimization of the double centrifugation method. This study uses experimental data obtained by researchers at the Clinical Pathology Laboratory of Dr. Soetomo Hospital, Surabaya. Experiments generally were conducted on stored blood obtained from the blood bag from PMI with CPD anticoagulant. Optimization of double centrifugation method to produce PRP with optimum platelet count could be obtained by using an "I"-shaped tube as well as other specialized equipment which are currently not available in standard clinical pathology laboratory, and also fresh blood. The resulting increase amounted to 4.11 times with the platelet count of 1.152 million.
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spelling doaj.art-9f31b73c9d1c43b19e20b5d94998a5602022-12-22T02:38:49ZengUniversitas AirlanggaFolia Medica Indonesiana2355-83932599-056X2016-11-0151318018210.20473/fmi.v51i3.28322028OPTIMATION OF PLATELET RICH PLASMA PRODUCTION PROTOCOL USING DOUBLE CENTRIFUGATION METHODSHans Kristian NugrahaYetti HernaningsihJusak NugrahaCurrently, therapy with Platelet Rich Plasma (PRP) has been widely used and continues to grow for various clinical applications. Along with its development, there are various options in the method of obtaining PRP, either automatic or manual, while one of the most reliable according to the literature is a double centrifugation method. The purpose of this research is to produce an optimization of the double centrifugation method. This study uses experimental data obtained by researchers at the Clinical Pathology Laboratory of Dr. Soetomo Hospital, Surabaya. Experiments generally were conducted on stored blood obtained from the blood bag from PMI with CPD anticoagulant. Optimization of double centrifugation method to produce PRP with optimum platelet count could be obtained by using an "I"-shaped tube as well as other specialized equipment which are currently not available in standard clinical pathology laboratory, and also fresh blood. The resulting increase amounted to 4.11 times with the platelet count of 1.152 million.https://e-journal.unair.ac.id/FMI/article/view/2832platelet rich plasma, double centrifugation method
spellingShingle Hans Kristian Nugraha
Yetti Hernaningsih
Jusak Nugraha
OPTIMATION OF PLATELET RICH PLASMA PRODUCTION PROTOCOL USING DOUBLE CENTRIFUGATION METHODS
Folia Medica Indonesiana
platelet rich plasma, double centrifugation method
title OPTIMATION OF PLATELET RICH PLASMA PRODUCTION PROTOCOL USING DOUBLE CENTRIFUGATION METHODS
title_full OPTIMATION OF PLATELET RICH PLASMA PRODUCTION PROTOCOL USING DOUBLE CENTRIFUGATION METHODS
title_fullStr OPTIMATION OF PLATELET RICH PLASMA PRODUCTION PROTOCOL USING DOUBLE CENTRIFUGATION METHODS
title_full_unstemmed OPTIMATION OF PLATELET RICH PLASMA PRODUCTION PROTOCOL USING DOUBLE CENTRIFUGATION METHODS
title_short OPTIMATION OF PLATELET RICH PLASMA PRODUCTION PROTOCOL USING DOUBLE CENTRIFUGATION METHODS
title_sort optimation of platelet rich plasma production protocol using double centrifugation methods
topic platelet rich plasma, double centrifugation method
url https://e-journal.unair.ac.id/FMI/article/view/2832
work_keys_str_mv AT hanskristiannugraha optimationofplateletrichplasmaproductionprotocolusingdoublecentrifugationmethods
AT yettihernaningsih optimationofplateletrichplasmaproductionprotocolusingdoublecentrifugationmethods
AT jusaknugraha optimationofplateletrichplasmaproductionprotocolusingdoublecentrifugationmethods