Unripe Citrus unshiu peel inhibited pre-adipocyte’s differentiation via leptin-PPARγ/FAS pathway and pro-inflammatory cytokines’ release
By 2030 the obese is prospected 20% of female and 14% of male and obesity induces many diseases. The active compounds in unripe C. unshiu peels were defined and anti-obesity effect was evaluated using with 3T3-L1 cell and high-fat diet mouse. In cell study the anti-obesity effect was analyzed using...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623002815 |
| _version_ | 1797749189587763200 |
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| author | Soon-Young Lee Min-Hee Kim Chun-Sik Bae Hyuk Joon Choi Eui Hyoun Ma Sung-Jin Park Seung-Sik Cho Dae-Hun Park |
| author_facet | Soon-Young Lee Min-Hee Kim Chun-Sik Bae Hyuk Joon Choi Eui Hyoun Ma Sung-Jin Park Seung-Sik Cho Dae-Hun Park |
| author_sort | Soon-Young Lee |
| collection | DOAJ |
| description | By 2030 the obese is prospected 20% of female and 14% of male and obesity induces many diseases. The active compounds in unripe C. unshiu peels were defined and anti-obesity effect was evaluated using with 3T3-L1 cell and high-fat diet mouse. In cell study the anti-obesity effect was analyzed using with oil red O stain and western blot and in animal study that was analyzed using with blood chemistry analysis, H&E stain, immunohistochemistry, RT-PCR, and ELISA. Thirty percentage ethanol extract of C. unshiu peels most effectively inhibited preadipocyte’s differentiation via PPARγ-C/EBPα pathway. In animal study it decreased the levels of LDL and leptin, suppressed fat accumulation, prevented adipogenesis via controlling PPARγ-C/EBPα-FAS, and inhibited inflammation via down-regulation of IL-1β, IL-13, and TNF-α. C. unshiu is a promising candidate for anti-obesity medication and for anti-inflammatory drug via regulating the level of leptin and the active compounds are supposed narirutin and hesperidin. |
| first_indexed | 2024-03-12T16:15:42Z |
| format | Article |
| id | doaj.art-9f3852fbf6294842acd9ad1d6d25e5af |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-12T16:15:42Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-9f3852fbf6294842acd9ad1d6d25e5af2023-08-09T04:32:35ZengElsevierJournal of Functional Foods1756-46462023-08-01107105681Unripe Citrus unshiu peel inhibited pre-adipocyte’s differentiation via leptin-PPARγ/FAS pathway and pro-inflammatory cytokines’ releaseSoon-Young Lee0Min-Hee Kim1Chun-Sik Bae2Hyuk Joon Choi3Eui Hyoun Ma4Sung-Jin Park5Seung-Sik Cho6Dae-Hun Park7College of Korean Medicine, Dongshin University, Naju, Jeonnam 58245, Republic of KoreaDepartment of Forestry and Landscape Architecture, Dongshin University, Naju, Jeonnam 58245, Republic of KoreaCollege of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of KoreaBKBIO, Jeju, Jeju-do 63359, Republic of KoreaBKBIO, Jeju, Jeju-do 63359, Republic of KoreaResearch Institute for Advanced Industrial Technology, Korea University, Sejong 30019, Republic of KoreaDepartment of Pharmacy, College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, 1666, Yeongsan-ro, Cheonggye-myeon, Muan, Jeonnam 58554, Republic of Korea; Biomedicine, Health & Life Convergence Sciences, BK21 Four, College of Pharmacy, Mokpo National University, 1666, Yeongsan-ro, Cheonggye-myeon, Muan, Jeonnam 58554, Republic of Korea; Corresponding authors at: Department of Pharmacy, College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, 1666, Yeongsan-ro, Cheonggye-myeon, Muan, Jeonnam 58554, Republic of Korea (S.-S. Cho). College of Oriental Medicine, Dongshin University, 185 Kunjae-ro, Naju, Jeonnam 58245, Republic of Korea (D.-H. Park).College of Korean Medicine, Dongshin University, Naju, Jeonnam 58245, Republic of Korea; Corresponding authors at: Department of Pharmacy, College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, 1666, Yeongsan-ro, Cheonggye-myeon, Muan, Jeonnam 58554, Republic of Korea (S.-S. Cho). College of Oriental Medicine, Dongshin University, 185 Kunjae-ro, Naju, Jeonnam 58245, Republic of Korea (D.-H. Park).By 2030 the obese is prospected 20% of female and 14% of male and obesity induces many diseases. The active compounds in unripe C. unshiu peels were defined and anti-obesity effect was evaluated using with 3T3-L1 cell and high-fat diet mouse. In cell study the anti-obesity effect was analyzed using with oil red O stain and western blot and in animal study that was analyzed using with blood chemistry analysis, H&E stain, immunohistochemistry, RT-PCR, and ELISA. Thirty percentage ethanol extract of C. unshiu peels most effectively inhibited preadipocyte’s differentiation via PPARγ-C/EBPα pathway. In animal study it decreased the levels of LDL and leptin, suppressed fat accumulation, prevented adipogenesis via controlling PPARγ-C/EBPα-FAS, and inhibited inflammation via down-regulation of IL-1β, IL-13, and TNF-α. C. unshiu is a promising candidate for anti-obesity medication and for anti-inflammatory drug via regulating the level of leptin and the active compounds are supposed narirutin and hesperidin.http://www.sciencedirect.com/science/article/pii/S1756464623002815C. unshiuNarirutinHesperidinAnti-obesityAnti-inflammationPPARγ-C/EBPα-FAS |
| spellingShingle | Soon-Young Lee Min-Hee Kim Chun-Sik Bae Hyuk Joon Choi Eui Hyoun Ma Sung-Jin Park Seung-Sik Cho Dae-Hun Park Unripe Citrus unshiu peel inhibited pre-adipocyte’s differentiation via leptin-PPARγ/FAS pathway and pro-inflammatory cytokines’ release Journal of Functional Foods C. unshiu Narirutin Hesperidin Anti-obesity Anti-inflammation PPARγ-C/EBPα-FAS |
| title | Unripe Citrus unshiu peel inhibited pre-adipocyte’s differentiation via leptin-PPARγ/FAS pathway and pro-inflammatory cytokines’ release |
| title_full | Unripe Citrus unshiu peel inhibited pre-adipocyte’s differentiation via leptin-PPARγ/FAS pathway and pro-inflammatory cytokines’ release |
| title_fullStr | Unripe Citrus unshiu peel inhibited pre-adipocyte’s differentiation via leptin-PPARγ/FAS pathway and pro-inflammatory cytokines’ release |
| title_full_unstemmed | Unripe Citrus unshiu peel inhibited pre-adipocyte’s differentiation via leptin-PPARγ/FAS pathway and pro-inflammatory cytokines’ release |
| title_short | Unripe Citrus unshiu peel inhibited pre-adipocyte’s differentiation via leptin-PPARγ/FAS pathway and pro-inflammatory cytokines’ release |
| title_sort | unripe citrus unshiu peel inhibited pre adipocyte s differentiation via leptin pparγ fas pathway and pro inflammatory cytokines release |
| topic | C. unshiu Narirutin Hesperidin Anti-obesity Anti-inflammation PPARγ-C/EBPα-FAS |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623002815 |
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