Zhilong Huoxue Tongyu Capsules' Effects on ischemic stroke: An assessment using fecal 16S rRNA gene sequencing and untargeted serum metabolomics
Zhilong Huoxue Tongyu capsule (ZHTC) is an effective traditional Chinese medicine compound for the treatment of ischemic stroke, which is widely used in clinical ischemic stroke patients. However, it is uncertain whether ZHTC affects ischemic stroke through gut microbiota and serum metabolites. In t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.1052110/full |
| _version_ | 1811318143670288384 |
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| author | Raoqiong Wang Raoqiong Wang Mengnan Liu Mengnan Liu Guilin Ren Guilin Ren Gang Luo Gang Luo Zhichuan Wang Zhengxin Ge Qingrong Pu Qingrong Pu Wei Ren Wei Ren Sijin Yang Sijin Yang |
| author_facet | Raoqiong Wang Raoqiong Wang Mengnan Liu Mengnan Liu Guilin Ren Guilin Ren Gang Luo Gang Luo Zhichuan Wang Zhengxin Ge Qingrong Pu Qingrong Pu Wei Ren Wei Ren Sijin Yang Sijin Yang |
| author_sort | Raoqiong Wang |
| collection | DOAJ |
| description | Zhilong Huoxue Tongyu capsule (ZHTC) is an effective traditional Chinese medicine compound for the treatment of ischemic stroke, which is widely used in clinical ischemic stroke patients. However, it is uncertain whether ZHTC affects ischemic stroke through gut microbiota and serum metabolites. In this study, a rat model of middle cerebral artery occlusion (MCAO) was prepared. By evaluating motor nerve function score, cerebral infarct size, brain tissue damage and intestinal barrier damage, it was found that ZHTC improved stroke-related symptoms in MCAO rats. Using 16S rRNA gene sequencing, fecal microbial transplantation (FMT), untargeted metabolomics, and spearman correlation analysis of gut microbiota and serum metabolites, we found that ZHTC can regulate the abundance of p_Firmicutes, p_Bacteroidota,p_Proteobacteria, g_Prevotella, and g_Lactobacillus, and regulated 23 differential metabolites. Spearman correlation analysis found that Arginine was positively correlated with p_Firmicutes, o_Clostridiales, c_Clostridia, and negatively correlated with p_Bacteroidetes, c_Bacteroidia,o_Bacteroidales; L-Lysine was negatively correlated with f_Christensenellaceae; L-methionine was positively correlated with o_Lactobacillales, f_Lactobacillaceae, and g_Lactobacillus. Altogether, this study shows for the first time that ZHTC can ameliorate ischemic stroke by modulating gut microbiota and metabolic disturbances. This lays the foundation for further revealing the causal relationship between ZHTC, gut dysbiosis, plasma metabolite levels and ischemic stroke, and provides a scientific explanation for the ameliorating effect of ZHTC on ischemic stroke. |
| first_indexed | 2024-04-13T12:21:44Z |
| format | Article |
| id | doaj.art-9f39333198fe4456814e6aac3244d214 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-13T12:21:44Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-9f39333198fe4456814e6aac3244d2142022-12-22T02:47:12ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-11-011310.3389/fphar.2022.10521101052110Zhilong Huoxue Tongyu Capsules' Effects on ischemic stroke: An assessment using fecal 16S rRNA gene sequencing and untargeted serum metabolomicsRaoqiong Wang0Raoqiong Wang1Mengnan Liu2Mengnan Liu3Guilin Ren4Guilin Ren5Gang Luo6Gang Luo7Zhichuan Wang8Zhengxin Ge9Qingrong Pu10Qingrong Pu11Wei Ren12Wei Ren13Sijin Yang14Sijin Yang15National Traditional Chinese Medicine Clinical Research Base, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, ChinaInstitute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, ChinaNational Traditional Chinese Medicine Clinical Research Base, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, ChinaInstitute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, ChinaNational Traditional Chinese Medicine Clinical Research Base, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, ChinaInstitute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, ChinaNational Traditional Chinese Medicine Clinical Research Base, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, ChinaInstitute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, ChinaInstitute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, ChinaInstitute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, ChinaNational Traditional Chinese Medicine Clinical Research Base, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, ChinaInstitute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, ChinaNational Traditional Chinese Medicine Clinical Research Base, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, ChinaInstitute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, ChinaNational Traditional Chinese Medicine Clinical Research Base, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, ChinaInstitute of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, ChinaZhilong Huoxue Tongyu capsule (ZHTC) is an effective traditional Chinese medicine compound for the treatment of ischemic stroke, which is widely used in clinical ischemic stroke patients. However, it is uncertain whether ZHTC affects ischemic stroke through gut microbiota and serum metabolites. In this study, a rat model of middle cerebral artery occlusion (MCAO) was prepared. By evaluating motor nerve function score, cerebral infarct size, brain tissue damage and intestinal barrier damage, it was found that ZHTC improved stroke-related symptoms in MCAO rats. Using 16S rRNA gene sequencing, fecal microbial transplantation (FMT), untargeted metabolomics, and spearman correlation analysis of gut microbiota and serum metabolites, we found that ZHTC can regulate the abundance of p_Firmicutes, p_Bacteroidota,p_Proteobacteria, g_Prevotella, and g_Lactobacillus, and regulated 23 differential metabolites. Spearman correlation analysis found that Arginine was positively correlated with p_Firmicutes, o_Clostridiales, c_Clostridia, and negatively correlated with p_Bacteroidetes, c_Bacteroidia,o_Bacteroidales; L-Lysine was negatively correlated with f_Christensenellaceae; L-methionine was positively correlated with o_Lactobacillales, f_Lactobacillaceae, and g_Lactobacillus. Altogether, this study shows for the first time that ZHTC can ameliorate ischemic stroke by modulating gut microbiota and metabolic disturbances. This lays the foundation for further revealing the causal relationship between ZHTC, gut dysbiosis, plasma metabolite levels and ischemic stroke, and provides a scientific explanation for the ameliorating effect of ZHTC on ischemic stroke.https://www.frontiersin.org/articles/10.3389/fphar.2022.1052110/fullischemic strokeintestinal floratraditional Chinese medicineZhiLong HuoXue TongYu capsulefecal microbial transplantationuntargeted serum metabolomics |
| spellingShingle | Raoqiong Wang Raoqiong Wang Mengnan Liu Mengnan Liu Guilin Ren Guilin Ren Gang Luo Gang Luo Zhichuan Wang Zhengxin Ge Qingrong Pu Qingrong Pu Wei Ren Wei Ren Sijin Yang Sijin Yang Zhilong Huoxue Tongyu Capsules' Effects on ischemic stroke: An assessment using fecal 16S rRNA gene sequencing and untargeted serum metabolomics Frontiers in Pharmacology ischemic stroke intestinal flora traditional Chinese medicine ZhiLong HuoXue TongYu capsule fecal microbial transplantation untargeted serum metabolomics |
| title | Zhilong Huoxue Tongyu Capsules' Effects on ischemic stroke: An assessment using fecal 16S rRNA gene sequencing and untargeted serum metabolomics |
| title_full | Zhilong Huoxue Tongyu Capsules' Effects on ischemic stroke: An assessment using fecal 16S rRNA gene sequencing and untargeted serum metabolomics |
| title_fullStr | Zhilong Huoxue Tongyu Capsules' Effects on ischemic stroke: An assessment using fecal 16S rRNA gene sequencing and untargeted serum metabolomics |
| title_full_unstemmed | Zhilong Huoxue Tongyu Capsules' Effects on ischemic stroke: An assessment using fecal 16S rRNA gene sequencing and untargeted serum metabolomics |
| title_short | Zhilong Huoxue Tongyu Capsules' Effects on ischemic stroke: An assessment using fecal 16S rRNA gene sequencing and untargeted serum metabolomics |
| title_sort | zhilong huoxue tongyu capsules effects on ischemic stroke an assessment using fecal 16s rrna gene sequencing and untargeted serum metabolomics |
| topic | ischemic stroke intestinal flora traditional Chinese medicine ZhiLong HuoXue TongYu capsule fecal microbial transplantation untargeted serum metabolomics |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.1052110/full |
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