MCM10 expression is linked to cervical cancer aggressiveness
Cervical cancer screening is a challenge mainly in developing countries. In developed countries, both incidence and mortality rates have been decreasing due to well organized screening programs. One of the potential biomarkers being exploited are the minichromosome maintenance proteins (MCMs), which...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Molecular Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmmed.2023.1009903/full |
| _version_ | 1797896064828702720 |
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| author | Sumayyah M. Q. Ahmed Suparna Laha Ranajit Das Mariam Anjum Ifthikar Shankar Prasad Das |
| author_facet | Sumayyah M. Q. Ahmed Suparna Laha Ranajit Das Mariam Anjum Ifthikar Shankar Prasad Das |
| author_sort | Sumayyah M. Q. Ahmed |
| collection | DOAJ |
| description | Cervical cancer screening is a challenge mainly in developing countries. In developed countries, both incidence and mortality rates have been decreasing due to well organized screening programs. One of the potential biomarkers being exploited are the minichromosome maintenance proteins (MCMs), which show both specificity and sensitivity. MCM2-7 are involved in DNA replication initiation and elongation, and the MCM subunits are highly expressed in malignant tissues. Unlike other MCMs, MCM10, which is not part of the core helicase complex, is a critical determinant of origin activation and its levels are limiting in cancer cells. In this study, we performed bioinformatic analysis on the expression profile of all DNA replication associated MCM proteins in cervical cancer. MCM10 showed a relatively higher expression profile compared to the other MCMs. The mRNA expression levels of the MCMs were significantly increased in tumour tissues compared to normal, and MCM10 showed a fold change of 3.4. In order to understand if MCM10 is associated with the aggressiveness of cervical cancer, we looked into the mRNA expression pattern of MCM10 in three cervical cancer cell lines and one normal cervical cell line. MCM10 expression was significantly higher in the case of the more aggressive cancer cell line HeLa compared to controls. MCM10, therefore, can serve as a prominent biomarker for cancer progression and thus aid in early detection to control the spread of cancer cells. Our results show that MCM10 expression levels in cervical cancer cell lines are associated with cancer aggressiveness, demonstrating its clinical significance. |
| first_indexed | 2024-04-10T07:35:43Z |
| format | Article |
| id | doaj.art-9f3aef5cc84d40438bef539154213dc1 |
| institution | Directory Open Access Journal |
| issn | 2674-0095 |
| language | English |
| last_indexed | 2024-04-10T07:35:43Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Medicine |
| spelling | doaj.art-9f3aef5cc84d40438bef539154213dc12023-02-23T12:46:21ZengFrontiers Media S.A.Frontiers in Molecular Medicine2674-00952023-02-01310.3389/fmmed.2023.10099031009903MCM10 expression is linked to cervical cancer aggressivenessSumayyah M. Q. Ahmed0Suparna Laha1Ranajit Das2Mariam Anjum Ifthikar3Shankar Prasad Das4Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore, IndiaYenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore, IndiaYenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore, IndiaDepartment of Oncology, Yenepoya Medical College Hospital, Yenepoya (Deemed to be University), Mangalore, IndiaYenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore, IndiaCervical cancer screening is a challenge mainly in developing countries. In developed countries, both incidence and mortality rates have been decreasing due to well organized screening programs. One of the potential biomarkers being exploited are the minichromosome maintenance proteins (MCMs), which show both specificity and sensitivity. MCM2-7 are involved in DNA replication initiation and elongation, and the MCM subunits are highly expressed in malignant tissues. Unlike other MCMs, MCM10, which is not part of the core helicase complex, is a critical determinant of origin activation and its levels are limiting in cancer cells. In this study, we performed bioinformatic analysis on the expression profile of all DNA replication associated MCM proteins in cervical cancer. MCM10 showed a relatively higher expression profile compared to the other MCMs. The mRNA expression levels of the MCMs were significantly increased in tumour tissues compared to normal, and MCM10 showed a fold change of 3.4. In order to understand if MCM10 is associated with the aggressiveness of cervical cancer, we looked into the mRNA expression pattern of MCM10 in three cervical cancer cell lines and one normal cervical cell line. MCM10 expression was significantly higher in the case of the more aggressive cancer cell line HeLa compared to controls. MCM10, therefore, can serve as a prominent biomarker for cancer progression and thus aid in early detection to control the spread of cancer cells. Our results show that MCM10 expression levels in cervical cancer cell lines are associated with cancer aggressiveness, demonstrating its clinical significance.https://www.frontiersin.org/articles/10.3389/fmmed.2023.1009903/fullcervical cancerminichromosome maintenance protein 10DNA replicationorigin firingcancer aggressivenessqRT-PCR |
| spellingShingle | Sumayyah M. Q. Ahmed Suparna Laha Ranajit Das Mariam Anjum Ifthikar Shankar Prasad Das MCM10 expression is linked to cervical cancer aggressiveness Frontiers in Molecular Medicine cervical cancer minichromosome maintenance protein 10 DNA replication origin firing cancer aggressiveness qRT-PCR |
| title | MCM10 expression is linked to cervical cancer aggressiveness |
| title_full | MCM10 expression is linked to cervical cancer aggressiveness |
| title_fullStr | MCM10 expression is linked to cervical cancer aggressiveness |
| title_full_unstemmed | MCM10 expression is linked to cervical cancer aggressiveness |
| title_short | MCM10 expression is linked to cervical cancer aggressiveness |
| title_sort | mcm10 expression is linked to cervical cancer aggressiveness |
| topic | cervical cancer minichromosome maintenance protein 10 DNA replication origin firing cancer aggressiveness qRT-PCR |
| url | https://www.frontiersin.org/articles/10.3389/fmmed.2023.1009903/full |
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