بررسی اثر کرم نانوامولسیونی حاوی اسانس لاواند و عصاره‌ی شیرین بیان بر بهبود زخم پوستی عمیق در موش صحرایی
Background: Despite the very good medicinal properties of lavender essential oil and licorice extract, some factors, such as allergic reaction and being biodegradable, limit the application of them as candidates for pharmacotherapeutic treatments. Nano-emulsification is one of the ways to overcome t...
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2019-01-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/10430 |
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| author | Maryam Kazemi Mohammad Hossein Aarabi Esmat Aghadavoud Sayyed Alireza Talaei |
| author_facet | Maryam Kazemi Mohammad Hossein Aarabi Esmat Aghadavoud Sayyed Alireza Talaei |
| author_sort | Maryam Kazemi |
| collection | DOAJ |
| description | Background: Despite the very good medicinal properties of lavender essential oil and licorice extract, some factors, such as allergic reaction and being biodegradable, limit the application of them as candidates for pharmacotherapeutic treatments. Nano-emulsification is one of the ways to overcome these limitations. The aim of this study was to investigate the effects of nanoemulsion cream containing lavender essential oil and licorice extract on healing of deep skin wound in rat model.
Methods: In this experimental study, nanoemulsion cream containing lavender essential oil and licorice extract was made using self-emulsifying method. To investigate its effect on wound healing process, a full-thickness skin wound was produced on 85 male Wistar rats. The wound area was digitally photographed at 2nd, 5th, 7th, 10th, and 14th days after surgery using a digital camera; then, the area was quantified using an image analysis system (Image J). Moreover, the expression of transforming growth factor-beta1 (TGF-β1), type I collagen (Col I), and Col III genes was evaluated using real-time polymerase chain reaction (real-time PCR) at days 2, 7, and 14.
Findings: Nanoemulsion cream significantly decreased the surface area of the wounds faster than lavender essential oil and licorice extract cream (P < 0.010) and phenytoin (P < 0.050). Moreover, real-time PCR showed that topical treatment of wounds with nanomulsion cream increased the expression of TGF-β1, Col I, and Col III genes in rat’s skin (P < 0.01).
Conclusion: Nanoemulsion cream containing lavender essential oil and licorice extract exhibits a promising wound healing potential towards excisional wound models in rats. |
| first_indexed | 2024-03-12T08:19:54Z |
| format | Article |
| id | doaj.art-9f3f70c22dce44c3a62820fb743769e2 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T08:19:54Z |
| publishDate | 2019-01-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-9f3f70c22dce44c3a62820fb743769e22023-09-02T18:35:27ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2019-01-01365031355136110.22122/jims.v36i503.104303253بررسی اثر کرم نانوامولسیونی حاوی اسانس لاواند و عصاره‌ی شیرین بیان بر بهبود زخم پوستی عمیق در موش صحراییMaryam Kazemi0Mohammad Hossein Aarabi1Esmat Aghadavoud2Sayyed Alireza Talaei3MSc Student, Biochemistry and Nutrition in Metabolic Diseases Research Center, Kashan University of Medical Sciences, Kashan, IranAssociate Professor, Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Biochemistry and Nutrition in Metabolic Diseases Research Center, Kashan University of Medical Sciences, Kashan, IranAssistant Professor, Physiology Research Center, Kashan University of Medical Sciences, Kashan, IranBackground: Despite the very good medicinal properties of lavender essential oil and licorice extract, some factors, such as allergic reaction and being biodegradable, limit the application of them as candidates for pharmacotherapeutic treatments. Nano-emulsification is one of the ways to overcome these limitations. The aim of this study was to investigate the effects of nanoemulsion cream containing lavender essential oil and licorice extract on healing of deep skin wound in rat model. Methods: In this experimental study, nanoemulsion cream containing lavender essential oil and licorice extract was made using self-emulsifying method. To investigate its effect on wound healing process, a full-thickness skin wound was produced on 85 male Wistar rats. The wound area was digitally photographed at 2nd, 5th, 7th, 10th, and 14th days after surgery using a digital camera; then, the area was quantified using an image analysis system (Image J). Moreover, the expression of transforming growth factor-beta1 (TGF-β1), type I collagen (Col I), and Col III genes was evaluated using real-time polymerase chain reaction (real-time PCR) at days 2, 7, and 14. Findings: Nanoemulsion cream significantly decreased the surface area of the wounds faster than lavender essential oil and licorice extract cream (P < 0.010) and phenytoin (P < 0.050). Moreover, real-time PCR showed that topical treatment of wounds with nanomulsion cream increased the expression of TGF-β1, Col I, and Col III genes in rat’s skin (P < 0.01). Conclusion: Nanoemulsion cream containing lavender essential oil and licorice extract exhibits a promising wound healing potential towards excisional wound models in rats.http://jims.mui.ac.ir/index.php/jims/article/view/10430Wound healingLavandula angustifoliaGlycyrrhiza glabra |
| spellingShingle | Maryam Kazemi Mohammad Hossein Aarabi Esmat Aghadavoud Sayyed Alireza Talaei بررسی اثر کرم نانوامولسیونی حاوی اسانس لاواند و عصاره‌ی شیرین بیان بر بهبود زخم پوستی عمیق در موش صحرایی مجله دانشکده پزشکی اصفهان Wound healing Lavandula angustifolia Glycyrrhiza glabra |
| title | بررسی اثر کرم نانوامولسیونی حاوی اسانس لاواند و عصاره‌ی شیرین بیان بر بهبود زخم پوستی عمیق در موش صحرایی |
| title_full | بررسی اثر کرم نانوامولسیونی حاوی اسانس لاواند و عصاره‌ی شیرین بیان بر بهبود زخم پوستی عمیق در موش صحرایی |
| title_fullStr | بررسی اثر کرم نانوامولسیونی حاوی اسانس لاواند و عصاره‌ی شیرین بیان بر بهبود زخم پوستی عمیق در موش صحرایی |
| title_full_unstemmed | بررسی اثر کرم نانوامولسیونی حاوی اسانس لاواند و عصاره‌ی شیرین بیان بر بهبود زخم پوستی عمیق در موش صحرایی |
| title_short | بررسی اثر کرم نانوامولسیونی حاوی اسانس لاواند و عصاره‌ی شیرین بیان بر بهبود زخم پوستی عمیق در موش صحرایی |
| title_sort | بررسی اثر کرم نانوامولسیونی حاوی اسانس لاواند و عصاره‌ی شیرین بیان بر بهبود زخم پوستی عمیق در موش صحرایی |
| topic | Wound healing Lavandula angustifolia Glycyrrhiza glabra |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/10430 |
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