Hepatitis C virus genotypes in blood donors from the Federal District, Central Brazil
The objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors from the Federal District, Central Brazil, and to compare HCV screening by serological assays and reverse transcriptase polymerase chain reaction (RT-PCR). Plasma samples from 57 individuals with reacti...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2004-12-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000800019 |
| _version_ | 1827840664731123712 |
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| author | Regina MS Amorim Claudiner P Oliveira Patrícia S Wyant Daniela M Cerqueira Geni NL Câmara Luciano S Flores Regina MB Martins Cláudia RF Martins |
| author_facet | Regina MS Amorim Claudiner P Oliveira Patrícia S Wyant Daniela M Cerqueira Geni NL Câmara Luciano S Flores Regina MB Martins Cláudia RF Martins |
| author_sort | Regina MS Amorim |
| collection | DOAJ |
| description | The objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors from the Federal District, Central Brazil, and to compare HCV screening by serological assays and reverse transcriptase polymerase chain reaction (RT-PCR). Plasma samples from 57 individuals with reactive or indeterminate results in serological anti-HCV screening assays (ELISA or EIA) were tested for HCV RNA by RT-PCR. The results from a confirmatory LIA serological assay were also evaluated. The 5' non-coding region of the HCV genome was amplified from 41 PCR positive samples (71.9%), which were further characterized by nucleotide sequencing analysis. Of these, 60.9% were of HCV genotype 1 and 39.1% of genotype 3. |
| first_indexed | 2024-03-12T07:37:32Z |
| format | Article |
| id | doaj.art-9f41c2d56ca8473cafda4361015d7ce9 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:37:32Z |
| publishDate | 2004-12-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-9f41c2d56ca8473cafda4361015d7ce92023-09-02T21:28:07ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602004-12-0199889589710.1590/S0074-02762004000800019Hepatitis C virus genotypes in blood donors from the Federal District, Central BrazilRegina MS AmorimClaudiner P OliveiraPatrícia S WyantDaniela M CerqueiraGeni NL CâmaraLuciano S FloresRegina MB MartinsCláudia RF MartinsThe objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors from the Federal District, Central Brazil, and to compare HCV screening by serological assays and reverse transcriptase polymerase chain reaction (RT-PCR). Plasma samples from 57 individuals with reactive or indeterminate results in serological anti-HCV screening assays (ELISA or EIA) were tested for HCV RNA by RT-PCR. The results from a confirmatory LIA serological assay were also evaluated. The 5' non-coding region of the HCV genome was amplified from 41 PCR positive samples (71.9%), which were further characterized by nucleotide sequencing analysis. Of these, 60.9% were of HCV genotype 1 and 39.1% of genotype 3.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000800019hepatitis C virusgenotypesFederal District, Central Brazil |
| spellingShingle | Regina MS Amorim Claudiner P Oliveira Patrícia S Wyant Daniela M Cerqueira Geni NL Câmara Luciano S Flores Regina MB Martins Cláudia RF Martins Hepatitis C virus genotypes in blood donors from the Federal District, Central Brazil Memorias do Instituto Oswaldo Cruz hepatitis C virus genotypes Federal District, Central Brazil |
| title | Hepatitis C virus genotypes in blood donors from the Federal District, Central Brazil |
| title_full | Hepatitis C virus genotypes in blood donors from the Federal District, Central Brazil |
| title_fullStr | Hepatitis C virus genotypes in blood donors from the Federal District, Central Brazil |
| title_full_unstemmed | Hepatitis C virus genotypes in blood donors from the Federal District, Central Brazil |
| title_short | Hepatitis C virus genotypes in blood donors from the Federal District, Central Brazil |
| title_sort | hepatitis c virus genotypes in blood donors from the federal district central brazil |
| topic | hepatitis C virus genotypes Federal District, Central Brazil |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000800019 |
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