Detection and quantification of Leptospira interrogans in hamster and rat kidney samples: immunofluorescent imprints versus real-time PCR.

Detection and quantification of Leptospira interrogans in hamster and rat kidney samples: immunofluorescent imprints versus real-time PCR.

A major limitation in the clinical management and experimental research of leptospirosis is the poor performance of the available methods for the direct detection of leptospires. In this study, we compared real-time PCR (qPCR), targeting the lipL32 gene, with the immunofluorescent imprint method (IM...

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Main Authors: Adenizar D Chagas-Junior, Caroline L R da Silva, Luciane Marieta Soares, Cleiton S Santos, Carlos D C M Silva, Daniel A Athanazio, Mitermayer G dos Reis, Flávia W Cruz McBride, Alan J A McBride
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3290571?pdf=render

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