A Combination of Two Probiotics, <i>Lactobacillus sporogenes</i> and <i>Clostridium butyricum</i>, Inhibits Colon Cancer Development: An In Vitro Study
Cancer remains a leading cause of death worldwide and, even though several advances have been made in terms of specific treatment, the late-stage detection and the associated side effects of the conventional drugs sustain the search for better treatment alternatives. Probiotics are live microorganis...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/9/1692 |
| _version_ | 1827658679798726656 |
|---|---|
| author | Oana Budu Christian Banciu Iulia Pinzaru Cristian Sarău Daniel Lighezan Codruța Șoica Cristina Dehelean George Drăghici Alina Dolghi Alexandra Prodea Marius Mioc |
| author_facet | Oana Budu Christian Banciu Iulia Pinzaru Cristian Sarău Daniel Lighezan Codruța Șoica Cristina Dehelean George Drăghici Alina Dolghi Alexandra Prodea Marius Mioc |
| author_sort | Oana Budu |
| collection | DOAJ |
| description | Cancer remains a leading cause of death worldwide and, even though several advances have been made in terms of specific treatment, the late-stage detection and the associated side effects of the conventional drugs sustain the search for better treatment alternatives. Probiotics are live microorganisms that have been proven to possess numerous health benefits for human hosts, including anticancer effects. In the present study, the in vitro effect of the association of two probiotic strains (PBT), <i>Lactobacillus sporogenes</i> and <i>Clostridium butyricum</i>, were tested against colon (HT-29 and HCT 116), lung (A549), and liver (HepG2) cancer cell lines, alone or in combination with 5-fluorouracil (5FU). Moreover, the underlying mechanism of PBT and PBT-5FU against the HT-29 cell line was evaluated using the Hoechst 33342 staining, revealing characteristic apoptotic modifications, such as chromatin condensation, nuclear fragmentation, and membrane blebbing. Furthermore, the increase in the expression of pro-apoptotic Bax, Bid, Bad, and Bak proteins and the inhibition of the anti-apoptotic Bcl-2 and Bcl-XL proteins were recorded. Collectively, these findings suggest that the two strains of probiotic bacteria, alone or in association with 5FU, induce apoptosis in colon cancer cells and may serve as a potential anticancer treatment. |
| first_indexed | 2024-03-09T23:07:05Z |
| format | Article |
| id | doaj.art-9f4d2a18305b4c2b88f96ee4a629effc |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T23:07:05Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-9f4d2a18305b4c2b88f96ee4a629effc2023-11-23T17:51:37ZengMDPI AGMicroorganisms2076-26072022-08-01109169210.3390/microorganisms10091692A Combination of Two Probiotics, <i>Lactobacillus sporogenes</i> and <i>Clostridium butyricum</i>, Inhibits Colon Cancer Development: An In Vitro StudyOana Budu0Christian Banciu1Iulia Pinzaru2Cristian Sarău3Daniel Lighezan4Codruța Șoica5Cristina Dehelean6George Drăghici7Alina Dolghi8Alexandra Prodea9Marius Mioc10Department of Internal Medicine IV, Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, RomaniaDepartment of Internal Medicine IV, Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, RomaniaDepartment of Toxicology and Drug Industry, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, RomaniaDepartment of Medical Semiology I, Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, RomaniaDepartment of Medical Semiology I, Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, RomaniaResearch Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaDepartment of Toxicology and Drug Industry, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, RomaniaDepartment of Toxicology and Drug Industry, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, RomaniaDepartment of Toxicology and Drug Industry, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, RomaniaResearch Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaResearch Centre for Pharmaco-Toxicological Evaluation, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Sq. No. 2, 300041 Timisoara, RomaniaCancer remains a leading cause of death worldwide and, even though several advances have been made in terms of specific treatment, the late-stage detection and the associated side effects of the conventional drugs sustain the search for better treatment alternatives. Probiotics are live microorganisms that have been proven to possess numerous health benefits for human hosts, including anticancer effects. In the present study, the in vitro effect of the association of two probiotic strains (PBT), <i>Lactobacillus sporogenes</i> and <i>Clostridium butyricum</i>, were tested against colon (HT-29 and HCT 116), lung (A549), and liver (HepG2) cancer cell lines, alone or in combination with 5-fluorouracil (5FU). Moreover, the underlying mechanism of PBT and PBT-5FU against the HT-29 cell line was evaluated using the Hoechst 33342 staining, revealing characteristic apoptotic modifications, such as chromatin condensation, nuclear fragmentation, and membrane blebbing. Furthermore, the increase in the expression of pro-apoptotic Bax, Bid, Bad, and Bak proteins and the inhibition of the anti-apoptotic Bcl-2 and Bcl-XL proteins were recorded. Collectively, these findings suggest that the two strains of probiotic bacteria, alone or in association with 5FU, induce apoptosis in colon cancer cells and may serve as a potential anticancer treatment.https://www.mdpi.com/2076-2607/10/9/1692probiotics5-fluorouracil<i>Lactobacillus sporogenes</i><i>Clostridium butyricum</i>colon cancerlung cancer |
| spellingShingle | Oana Budu Christian Banciu Iulia Pinzaru Cristian Sarău Daniel Lighezan Codruța Șoica Cristina Dehelean George Drăghici Alina Dolghi Alexandra Prodea Marius Mioc A Combination of Two Probiotics, <i>Lactobacillus sporogenes</i> and <i>Clostridium butyricum</i>, Inhibits Colon Cancer Development: An In Vitro Study Microorganisms probiotics 5-fluorouracil <i>Lactobacillus sporogenes</i> <i>Clostridium butyricum</i> colon cancer lung cancer |
| title | A Combination of Two Probiotics, <i>Lactobacillus sporogenes</i> and <i>Clostridium butyricum</i>, Inhibits Colon Cancer Development: An In Vitro Study |
| title_full | A Combination of Two Probiotics, <i>Lactobacillus sporogenes</i> and <i>Clostridium butyricum</i>, Inhibits Colon Cancer Development: An In Vitro Study |
| title_fullStr | A Combination of Two Probiotics, <i>Lactobacillus sporogenes</i> and <i>Clostridium butyricum</i>, Inhibits Colon Cancer Development: An In Vitro Study |
| title_full_unstemmed | A Combination of Two Probiotics, <i>Lactobacillus sporogenes</i> and <i>Clostridium butyricum</i>, Inhibits Colon Cancer Development: An In Vitro Study |
| title_short | A Combination of Two Probiotics, <i>Lactobacillus sporogenes</i> and <i>Clostridium butyricum</i>, Inhibits Colon Cancer Development: An In Vitro Study |
| title_sort | combination of two probiotics i lactobacillus sporogenes i and i clostridium butyricum i inhibits colon cancer development an in vitro study |
| topic | probiotics 5-fluorouracil <i>Lactobacillus sporogenes</i> <i>Clostridium butyricum</i> colon cancer lung cancer |
| url | https://www.mdpi.com/2076-2607/10/9/1692 |
| work_keys_str_mv | AT oanabudu acombinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT christianbanciu acombinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT iuliapinzaru acombinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT cristiansarau acombinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT daniellighezan acombinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT codrutasoica acombinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT cristinadehelean acombinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT georgedraghici acombinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT alinadolghi acombinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT alexandraprodea acombinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT mariusmioc acombinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT oanabudu combinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT christianbanciu combinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT iuliapinzaru combinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT cristiansarau combinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT daniellighezan combinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT codrutasoica combinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT cristinadehelean combinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT georgedraghici combinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT alinadolghi combinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT alexandraprodea combinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy AT mariusmioc combinationoftwoprobioticsilactobacillussporogenesiandiclostridiumbutyricumiinhibitscoloncancerdevelopmentaninvitrostudy |