Ribosome profiling: a powerful tool in oncological research
Abstract Neoplastic cells need to adapt their gene expression pattern to survive in an ever-changing or unfavorable tumor microenvironment. Protein synthesis (or mRNA translation), an essential part of gene expression, is dysregulated in cancer. The emergence of distinct translatomic technologies ha...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-01-01
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| Series: | Biomarker Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40364-024-00562-4 |
| _version_ | 1797276414549950464 |
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| author | Dan Su Chen Ding Jiangdong Qiu Gang Yang Ruobing Wang Yueze Liu Jinxin Tao Wenhao Luo Guihu Weng Taiping Zhang |
| author_facet | Dan Su Chen Ding Jiangdong Qiu Gang Yang Ruobing Wang Yueze Liu Jinxin Tao Wenhao Luo Guihu Weng Taiping Zhang |
| author_sort | Dan Su |
| collection | DOAJ |
| description | Abstract Neoplastic cells need to adapt their gene expression pattern to survive in an ever-changing or unfavorable tumor microenvironment. Protein synthesis (or mRNA translation), an essential part of gene expression, is dysregulated in cancer. The emergence of distinct translatomic technologies has revolutionized oncological studies to elucidate translational regulatory mechanisms. Ribosome profiling can provide adequate information on diverse aspects of translation by aiding in quantitatively analyzing the intensity of translating ribosome-protected fragments. Here, we review the primary currently used translatomics techniques and highlight their advantages and disadvantages as tools for translatomics studies. Subsequently, we clarified the areas in which ribosome profiling could be applied to better understand translational control. Finally, we summarized the latest advances in cancer studies using ribosome profiling to highlight the extensive application of this powerful and promising translatomic tool. |
| first_indexed | 2024-03-07T15:27:58Z |
| format | Article |
| id | doaj.art-9f4e6863f22343259a86251b6b7612cd |
| institution | Directory Open Access Journal |
| issn | 2050-7771 |
| language | English |
| last_indexed | 2024-03-07T15:27:58Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biomarker Research |
| spelling | doaj.art-9f4e6863f22343259a86251b6b7612cd2024-03-05T16:37:00ZengBMCBiomarker Research2050-77712024-01-0112112110.1186/s40364-024-00562-4Ribosome profiling: a powerful tool in oncological researchDan Su0Chen Ding1Jiangdong Qiu2Gang Yang3Ruobing Wang4Yueze Liu5Jinxin Tao6Wenhao Luo7Guihu Weng8Taiping Zhang9General Surgery Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical CollegeGeneral Surgery Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical CollegeGeneral Surgery Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical CollegeGeneral Surgery Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical CollegeGeneral Surgery Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical CollegeGeneral Surgery Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical CollegeGeneral Surgery Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical CollegeGeneral Surgery Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical CollegeKey Laboratory of Research in Pancreatic Tumor, Chinese Academy of Medical SciencesGeneral Surgery Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical CollegeAbstract Neoplastic cells need to adapt their gene expression pattern to survive in an ever-changing or unfavorable tumor microenvironment. Protein synthesis (or mRNA translation), an essential part of gene expression, is dysregulated in cancer. The emergence of distinct translatomic technologies has revolutionized oncological studies to elucidate translational regulatory mechanisms. Ribosome profiling can provide adequate information on diverse aspects of translation by aiding in quantitatively analyzing the intensity of translating ribosome-protected fragments. Here, we review the primary currently used translatomics techniques and highlight their advantages and disadvantages as tools for translatomics studies. Subsequently, we clarified the areas in which ribosome profiling could be applied to better understand translational control. Finally, we summarized the latest advances in cancer studies using ribosome profiling to highlight the extensive application of this powerful and promising translatomic tool.https://doi.org/10.1186/s40364-024-00562-4Ribosome profilingcancerTranslatomicsMetabolic reprogrammingTranslational rewiringOncological pathway |
| spellingShingle | Dan Su Chen Ding Jiangdong Qiu Gang Yang Ruobing Wang Yueze Liu Jinxin Tao Wenhao Luo Guihu Weng Taiping Zhang Ribosome profiling: a powerful tool in oncological research Biomarker Research Ribosome profiling cancer Translatomics Metabolic reprogramming Translational rewiring Oncological pathway |
| title | Ribosome profiling: a powerful tool in oncological research |
| title_full | Ribosome profiling: a powerful tool in oncological research |
| title_fullStr | Ribosome profiling: a powerful tool in oncological research |
| title_full_unstemmed | Ribosome profiling: a powerful tool in oncological research |
| title_short | Ribosome profiling: a powerful tool in oncological research |
| title_sort | ribosome profiling a powerful tool in oncological research |
| topic | Ribosome profiling cancer Translatomics Metabolic reprogramming Translational rewiring Oncological pathway |
| url | https://doi.org/10.1186/s40364-024-00562-4 |
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