Composition of Particulate Matter and Bacterial Community in Gut Contents and Surrounding Sediments of Three Sipunculan Species (<i>Siphonosoma australe</i>, <i>Phascolosoma arcuatum</i>, and <i>Sipunculus nudus</i>)
<i>Siphonosoma australe</i>, <i>Phascolosoma arcuatum,</i> and <i>Sipunculus nudus</i> are three important sipunculan species in tropical intertidal zones. In this study, the particle size, organic matter content, and bacterial community composition in the gut con...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/6/6001 |
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| author | Chunsheng Liu Chuang Liu Fei Gao Aimin Wang Haiqing Wang Yumei Yang Linwen He |
| author_facet | Chunsheng Liu Chuang Liu Fei Gao Aimin Wang Haiqing Wang Yumei Yang Linwen He |
| author_sort | Chunsheng Liu |
| collection | DOAJ |
| description | <i>Siphonosoma australe</i>, <i>Phascolosoma arcuatum,</i> and <i>Sipunculus nudus</i> are three important sipunculan species in tropical intertidal zones. In this study, the particle size, organic matter content, and bacterial community composition in the gut contents of three different sipunculans and their surrounding sediments were analyzed. The grain size fractions of sipunculans’ guts were significantly different from those of their surrounding sediments; particle size fractions < 500 μm were favored by the sipunculans. As for the total organic matter (TOM), higher contents of organic matter were observed in the guts than in the surrounding sediments in all three sipunculan species. The bacterial community composition of all the samples was investigated by 16S rRNA gene sequencing, in which a total of 8974 OTUs were obtained from 24 samples based on a 97% threshold. The predominant phylum identified from the gut contents of three sipunculans was Planctomycetota, while the predominant phylum in their surrounding sediments was Proteobacteria. At the genus level, the most abundant genus was <i>Sulfurovum</i> (average 4.36%) in the surrounding sediments, while the most abundant genus was <i>Gplla</i> (average 12.76%) in the gut contents. The UPGMA tree showed that the samples from the guts of three different sipunculans and their surrounding sediments were clustered separately into two groups, which showed that these three sipunculans had a different bacterial community composition with their surrounding sediments. The grain size and total organic matter (TOM) had the greatest impacts on the bacterial community composition at both the phylum and genus levels. In conclusion, the differences in particle size fractions, organic matter content, and bacterial community composition between the gut contents and surrounding sediments in these three sipunculan species might be caused by their selective ingestion. |
| first_indexed | 2024-03-11T06:24:15Z |
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| language | English |
| last_indexed | 2024-03-11T06:24:15Z |
| publishDate | 2023-03-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9f4f4afbdebd46d8afa3fa04979332552023-11-17T11:42:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246600110.3390/ijms24066001Composition of Particulate Matter and Bacterial Community in Gut Contents and Surrounding Sediments of Three Sipunculan Species (<i>Siphonosoma australe</i>, <i>Phascolosoma arcuatum</i>, and <i>Sipunculus nudus</i>)Chunsheng Liu0Chuang Liu1Fei Gao2Aimin Wang3Haiqing Wang4Yumei Yang5Linwen He6State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaCollege of Marine Science, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaCollege of Marine Science, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China<i>Siphonosoma australe</i>, <i>Phascolosoma arcuatum,</i> and <i>Sipunculus nudus</i> are three important sipunculan species in tropical intertidal zones. In this study, the particle size, organic matter content, and bacterial community composition in the gut contents of three different sipunculans and their surrounding sediments were analyzed. The grain size fractions of sipunculans’ guts were significantly different from those of their surrounding sediments; particle size fractions < 500 μm were favored by the sipunculans. As for the total organic matter (TOM), higher contents of organic matter were observed in the guts than in the surrounding sediments in all three sipunculan species. The bacterial community composition of all the samples was investigated by 16S rRNA gene sequencing, in which a total of 8974 OTUs were obtained from 24 samples based on a 97% threshold. The predominant phylum identified from the gut contents of three sipunculans was Planctomycetota, while the predominant phylum in their surrounding sediments was Proteobacteria. At the genus level, the most abundant genus was <i>Sulfurovum</i> (average 4.36%) in the surrounding sediments, while the most abundant genus was <i>Gplla</i> (average 12.76%) in the gut contents. The UPGMA tree showed that the samples from the guts of three different sipunculans and their surrounding sediments were clustered separately into two groups, which showed that these three sipunculans had a different bacterial community composition with their surrounding sediments. The grain size and total organic matter (TOM) had the greatest impacts on the bacterial community composition at both the phylum and genus levels. In conclusion, the differences in particle size fractions, organic matter content, and bacterial community composition between the gut contents and surrounding sediments in these three sipunculan species might be caused by their selective ingestion.https://www.mdpi.com/1422-0067/24/6/6001sipunculan wormsgrain sizetotal organic matter16S ribosomal RNAbacterial community composition |
| spellingShingle | Chunsheng Liu Chuang Liu Fei Gao Aimin Wang Haiqing Wang Yumei Yang Linwen He Composition of Particulate Matter and Bacterial Community in Gut Contents and Surrounding Sediments of Three Sipunculan Species (<i>Siphonosoma australe</i>, <i>Phascolosoma arcuatum</i>, and <i>Sipunculus nudus</i>) International Journal of Molecular Sciences sipunculan worms grain size total organic matter 16S ribosomal RNA bacterial community composition |
| title | Composition of Particulate Matter and Bacterial Community in Gut Contents and Surrounding Sediments of Three Sipunculan Species (<i>Siphonosoma australe</i>, <i>Phascolosoma arcuatum</i>, and <i>Sipunculus nudus</i>) |
| title_full | Composition of Particulate Matter and Bacterial Community in Gut Contents and Surrounding Sediments of Three Sipunculan Species (<i>Siphonosoma australe</i>, <i>Phascolosoma arcuatum</i>, and <i>Sipunculus nudus</i>) |
| title_fullStr | Composition of Particulate Matter and Bacterial Community in Gut Contents and Surrounding Sediments of Three Sipunculan Species (<i>Siphonosoma australe</i>, <i>Phascolosoma arcuatum</i>, and <i>Sipunculus nudus</i>) |
| title_full_unstemmed | Composition of Particulate Matter and Bacterial Community in Gut Contents and Surrounding Sediments of Three Sipunculan Species (<i>Siphonosoma australe</i>, <i>Phascolosoma arcuatum</i>, and <i>Sipunculus nudus</i>) |
| title_short | Composition of Particulate Matter and Bacterial Community in Gut Contents and Surrounding Sediments of Three Sipunculan Species (<i>Siphonosoma australe</i>, <i>Phascolosoma arcuatum</i>, and <i>Sipunculus nudus</i>) |
| title_sort | composition of particulate matter and bacterial community in gut contents and surrounding sediments of three sipunculan species i siphonosoma australe i i phascolosoma arcuatum i and i sipunculus nudus i |
| topic | sipunculan worms grain size total organic matter 16S ribosomal RNA bacterial community composition |
| url | https://www.mdpi.com/1422-0067/24/6/6001 |
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