Diclofenac Embedded in Silk Fibroin Fibers as a Drug Delivery System
Silk fibroin is a biocompatible, non-toxic, mechanically robust protein, and it is commonly used and studied as a material for biomedical applications. Silk fibroin also gained particular interest as a drug carrier vehicle, and numerous silk formats have been investigated for this purpose. Herein, w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/13/16/3580 |
| _version_ | 1797558395976286208 |
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| author | Alena Opálková Šišková Erika Kozma Andrej Opálek Zuzana Kroneková Angela Kleinová Štefan Nagy Juraj Kronek Joanna Rydz Anita Eckstein Andicsová |
| author_facet | Alena Opálková Šišková Erika Kozma Andrej Opálek Zuzana Kroneková Angela Kleinová Štefan Nagy Juraj Kronek Joanna Rydz Anita Eckstein Andicsová |
| author_sort | Alena Opálková Šišková |
| collection | DOAJ |
| description | Silk fibroin is a biocompatible, non-toxic, mechanically robust protein, and it is commonly used and studied as a material for biomedical applications. Silk fibroin also gained particular interest as a drug carrier vehicle, and numerous silk formats have been investigated for this purpose. Herein, we have prepared electrospun nanofibers from pure silk fibroin and blended silk fibroin/casein, followed by the incorporation of an anti-inflammatory drug, diclofenac. Casein serves as an excipient in pharmaceutical products and has a positive effect on the gradual release of drugs. The characteristics of the investigated composites were estimated by scanning electron microscope, transmission electron microscope, thermogravimetric analysis, and a lifetime of diclofenac by electron paramagnetic resonance analysis. The cumulative release in vitro of diclofenac sodium salt, together with the antiproliferative effect of diclofenac sodium salt-loaded silk nanofibers against the growth of two cancer cell lines, are presented and discussed. |
| first_indexed | 2024-03-10T17:29:54Z |
| format | Article |
| id | doaj.art-9f5650f1be7e49a99f961335e485d1e6 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T17:29:54Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-9f5650f1be7e49a99f961335e485d1e62023-11-20T10:03:09ZengMDPI AGMaterials1996-19442020-08-011316358010.3390/ma13163580Diclofenac Embedded in Silk Fibroin Fibers as a Drug Delivery SystemAlena Opálková Šišková0Erika Kozma1Andrej Opálek2Zuzana Kroneková3Angela Kleinová4Štefan Nagy5Juraj Kronek6Joanna Rydz7Anita Eckstein Andicsová8Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 84541 Bratislava, SlovakiaInstitute for Chemical Sciences and Technologies ‘Giulio Natta’ (SCITEC-CNR), Via A. Corti 12, 20133 Milan, ItalyInstitute of Materials and Machine Mechanics, Slovak Academy of Sciences, Dúbravská cesta 9, 84541 Bratislava, SlovakiaPolymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 84541 Bratislava, SlovakiaPolymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 84541 Bratislava, SlovakiaInstitute of Materials and Machine Mechanics, Slovak Academy of Sciences, Dúbravská cesta 9, 84541 Bratislava, SlovakiaPolymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 84541 Bratislava, SlovakiaCentre of Polymer and Carbon Materials, Polish Academy of Sciences, 34, M. Curie-Skłodowska St., 41-800 Zabrze, PolandPolymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 84541 Bratislava, SlovakiaSilk fibroin is a biocompatible, non-toxic, mechanically robust protein, and it is commonly used and studied as a material for biomedical applications. Silk fibroin also gained particular interest as a drug carrier vehicle, and numerous silk formats have been investigated for this purpose. Herein, we have prepared electrospun nanofibers from pure silk fibroin and blended silk fibroin/casein, followed by the incorporation of an anti-inflammatory drug, diclofenac. Casein serves as an excipient in pharmaceutical products and has a positive effect on the gradual release of drugs. The characteristics of the investigated composites were estimated by scanning electron microscope, transmission electron microscope, thermogravimetric analysis, and a lifetime of diclofenac by electron paramagnetic resonance analysis. The cumulative release in vitro of diclofenac sodium salt, together with the antiproliferative effect of diclofenac sodium salt-loaded silk nanofibers against the growth of two cancer cell lines, are presented and discussed.https://www.mdpi.com/1996-1944/13/16/3580silk fibroinnanofiberselectrospinningcaseindiclofenacdrug delivery system |
| spellingShingle | Alena Opálková Šišková Erika Kozma Andrej Opálek Zuzana Kroneková Angela Kleinová Štefan Nagy Juraj Kronek Joanna Rydz Anita Eckstein Andicsová Diclofenac Embedded in Silk Fibroin Fibers as a Drug Delivery System Materials silk fibroin nanofibers electrospinning casein diclofenac drug delivery system |
| title | Diclofenac Embedded in Silk Fibroin Fibers as a Drug Delivery System |
| title_full | Diclofenac Embedded in Silk Fibroin Fibers as a Drug Delivery System |
| title_fullStr | Diclofenac Embedded in Silk Fibroin Fibers as a Drug Delivery System |
| title_full_unstemmed | Diclofenac Embedded in Silk Fibroin Fibers as a Drug Delivery System |
| title_short | Diclofenac Embedded in Silk Fibroin Fibers as a Drug Delivery System |
| title_sort | diclofenac embedded in silk fibroin fibers as a drug delivery system |
| topic | silk fibroin nanofibers electrospinning casein diclofenac drug delivery system |
| url | https://www.mdpi.com/1996-1944/13/16/3580 |
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