Development and validation of spectrophotometric methods for simultaneous estimation of citicoline and piracetam in tablet dosage form
Context: Citicoline (CN) and piracetam (PM) combination in tablet formulation is newly introduced in market. It is necessary to develop suitable quality control methods for rapid and accurate determination of these drugs. Aim: The study aimed to develop the methods for simultaneous determination of...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2013-01-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
| Subjects: | |
| Online Access: | http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2013;volume=5;issue=3;spage=202;epage=207;aulast=Sivadas |
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| author | Akhila Sivadas Aiswarya Sathi Kavya Sathi Kalpana Pravin Rahate |
| author_facet | Akhila Sivadas Aiswarya Sathi Kavya Sathi Kalpana Pravin Rahate |
| author_sort | Akhila Sivadas |
| collection | DOAJ |
| description | Context: Citicoline (CN) and piracetam (PM) combination in tablet formulation is newly introduced in market. It is necessary to develop suitable quality control methods for rapid and accurate determination of these drugs. Aim: The study aimed to develop the methods for simultaneous determination of CN and PM in combined dosage form. Materials and Methods: The first method was developed by formation and solving simultaneous equations using 280.3 and 264.1 nm as two analytical wavelengths. Second method was absorbance ratio in which wavelengths selected were 256.6 nm as its absorptive point and 280.3 nm as λmax of CN. According to International Conference on Harmonization (ICH) norm, the parameters - linearity, precision, and accuracy were studied. The methods were validated statistically and by recovery studies. Results: Both the drugs obeyed Beer-Lambert′s law at the selected wavelengths in concentration range of 5-13 μg/ml for CN and 10-22 μg/ml for PM. The percentage of CN and PM in marketed tablet formulation was found to be 99.006 ± 0.173 and 99.257 ± 0.613, respectively; by simultaneous equation method. For Q-Absorption ratio method the percentage of CN and PM was found to be 99.078 ± 0.158 and 99.708 ± 0.838, respectively. Conclusions: The proposed methods were simple, reproducible, precise and robust. The methods can be successfully applied for routine analysis of tablets. |
| first_indexed | 2024-12-11T02:22:15Z |
| format | Article |
| id | doaj.art-9f57f7ecc74d47ed81f0d7187b0e065c |
| institution | Directory Open Access Journal |
| issn | 0975-7406 0976-4879 |
| language | English |
| last_indexed | 2024-12-11T02:22:15Z |
| publishDate | 2013-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj.art-9f57f7ecc74d47ed81f0d7187b0e065c2022-12-22T01:24:01ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0975-74060976-48792013-01-015320220710.4103/0975-7406.116818Development and validation of spectrophotometric methods for simultaneous estimation of citicoline and piracetam in tablet dosage formAkhila SivadasAiswarya SathiKavya SathiKalpana Pravin RahateContext: Citicoline (CN) and piracetam (PM) combination in tablet formulation is newly introduced in market. It is necessary to develop suitable quality control methods for rapid and accurate determination of these drugs. Aim: The study aimed to develop the methods for simultaneous determination of CN and PM in combined dosage form. Materials and Methods: The first method was developed by formation and solving simultaneous equations using 280.3 and 264.1 nm as two analytical wavelengths. Second method was absorbance ratio in which wavelengths selected were 256.6 nm as its absorptive point and 280.3 nm as λmax of CN. According to International Conference on Harmonization (ICH) norm, the parameters - linearity, precision, and accuracy were studied. The methods were validated statistically and by recovery studies. Results: Both the drugs obeyed Beer-Lambert′s law at the selected wavelengths in concentration range of 5-13 μg/ml for CN and 10-22 μg/ml for PM. The percentage of CN and PM in marketed tablet formulation was found to be 99.006 ± 0.173 and 99.257 ± 0.613, respectively; by simultaneous equation method. For Q-Absorption ratio method the percentage of CN and PM was found to be 99.078 ± 0.158 and 99.708 ± 0.838, respectively. Conclusions: The proposed methods were simple, reproducible, precise and robust. The methods can be successfully applied for routine analysis of tablets.http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2013;volume=5;issue=3;spage=202;epage=207;aulast=SivadasAbsorbance ratiociticolinepiracetamrecovery studysimultaneous spectrophotometryvalidation |
| spellingShingle | Akhila Sivadas Aiswarya Sathi Kavya Sathi Kalpana Pravin Rahate Development and validation of spectrophotometric methods for simultaneous estimation of citicoline and piracetam in tablet dosage form Journal of Pharmacy and Bioallied Sciences Absorbance ratio citicoline piracetam recovery study simultaneous spectrophotometry validation |
| title | Development and validation of spectrophotometric methods for simultaneous estimation of citicoline and piracetam in tablet dosage form |
| title_full | Development and validation of spectrophotometric methods for simultaneous estimation of citicoline and piracetam in tablet dosage form |
| title_fullStr | Development and validation of spectrophotometric methods for simultaneous estimation of citicoline and piracetam in tablet dosage form |
| title_full_unstemmed | Development and validation of spectrophotometric methods for simultaneous estimation of citicoline and piracetam in tablet dosage form |
| title_short | Development and validation of spectrophotometric methods for simultaneous estimation of citicoline and piracetam in tablet dosage form |
| title_sort | development and validation of spectrophotometric methods for simultaneous estimation of citicoline and piracetam in tablet dosage form |
| topic | Absorbance ratio citicoline piracetam recovery study simultaneous spectrophotometry validation |
| url | http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2013;volume=5;issue=3;spage=202;epage=207;aulast=Sivadas |
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