Assessment role of total phenols and flavonoids extracted from Pleurotus columbinus mushroom on the premature ovarian failure induced by chemotherapy in rats
Abstract Background Many species of mushroom contain an assortment of free radical scavengers (Phenolics and Flavonoids compounds) that have made them nutritionally beneficial and a source of expansion of drug production. In this study, we examined the preventive and remedial role of total phenol ex...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43141-021-00278-0 |
| _version_ | 1797203113069772800 |
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| author | Amal I. Hassan Mona A. M. Ghoneim Manal G. Mahmoud Mohsen S. Asker |
| author_facet | Amal I. Hassan Mona A. M. Ghoneim Manal G. Mahmoud Mohsen S. Asker |
| author_sort | Amal I. Hassan |
| collection | DOAJ |
| description | Abstract Background Many species of mushroom contain an assortment of free radical scavengers (Phenolics and Flavonoids compounds) that have made them nutritionally beneficial and a source of expansion of drug production. In this study, we examined the preventive and remedial role of total phenol extract from Pleurotus columbines (TP) in alleviating the consequences of cyclophosphamide (CTX) on the ovaries of female rats. Rats were randomly assigned to four groups: healthy controls, cyclophosphamide (CTX), received a TP (100 mg/kg) orally daily for 14 days and curative group: CTX-TP, we determined and identified a total phenol from a mushroom extract and examined it as an antioxidant agent. To investigate the therapeutic influence, it was administrated 2 weeks after CTX. To assess the impact of TP on ovarian damage caused by CTX, ovarian hormone tests were performed such as luteinizing hormone (LH), 17-β-estradiol (E2), and anti-mullerian hormone (AMH). Besides, follicle-stimulating hormone (FSH) in serum was evaluated, and histopathological analysis of the ovary was examined. Results This study indicates that treatment with TP decreased the severity of cyclophosphamide-induced ovary injury by reducing inflammation and apoptotic effects and increasing the activity of antioxidants. Conclusions TP could be used to alleviate cyclophosphamide-induced ovary injury. |
| first_indexed | 2024-04-24T08:14:10Z |
| format | Article |
| id | doaj.art-9f5ca39aa2104dda9ef0da3f228ab181 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:14:10Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-9f5ca39aa2104dda9ef0da3f228ab1812024-04-17T04:33:32ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202021-12-0119111110.1186/s43141-021-00278-0Assessment role of total phenols and flavonoids extracted from Pleurotus columbinus mushroom on the premature ovarian failure induced by chemotherapy in ratsAmal I. Hassan0Mona A. M. Ghoneim1Manal G. Mahmoud2Mohsen S. Asker3Department of Radioisotopes, Nuclear Research Centre, Egyptian Atomic Energy AuthorityDepartment of Radioisotopes, Nuclear Research Centre, Egyptian Atomic Energy AuthorityMicrobial Biotechnology Department, National Research CentreMicrobial Biotechnology Department, National Research CentreAbstract Background Many species of mushroom contain an assortment of free radical scavengers (Phenolics and Flavonoids compounds) that have made them nutritionally beneficial and a source of expansion of drug production. In this study, we examined the preventive and remedial role of total phenol extract from Pleurotus columbines (TP) in alleviating the consequences of cyclophosphamide (CTX) on the ovaries of female rats. Rats were randomly assigned to four groups: healthy controls, cyclophosphamide (CTX), received a TP (100 mg/kg) orally daily for 14 days and curative group: CTX-TP, we determined and identified a total phenol from a mushroom extract and examined it as an antioxidant agent. To investigate the therapeutic influence, it was administrated 2 weeks after CTX. To assess the impact of TP on ovarian damage caused by CTX, ovarian hormone tests were performed such as luteinizing hormone (LH), 17-β-estradiol (E2), and anti-mullerian hormone (AMH). Besides, follicle-stimulating hormone (FSH) in serum was evaluated, and histopathological analysis of the ovary was examined. Results This study indicates that treatment with TP decreased the severity of cyclophosphamide-induced ovary injury by reducing inflammation and apoptotic effects and increasing the activity of antioxidants. Conclusions TP could be used to alleviate cyclophosphamide-induced ovary injury.https://doi.org/10.1186/s43141-021-00278-0CyclophosphamideMushroomFlavonoidsTotal phenolsNatural antioxidant |
| spellingShingle | Amal I. Hassan Mona A. M. Ghoneim Manal G. Mahmoud Mohsen S. Asker Assessment role of total phenols and flavonoids extracted from Pleurotus columbinus mushroom on the premature ovarian failure induced by chemotherapy in rats Journal of Genetic Engineering and Biotechnology Cyclophosphamide Mushroom Flavonoids Total phenols Natural antioxidant |
| title | Assessment role of total phenols and flavonoids extracted from Pleurotus columbinus mushroom on the premature ovarian failure induced by chemotherapy in rats |
| title_full | Assessment role of total phenols and flavonoids extracted from Pleurotus columbinus mushroom on the premature ovarian failure induced by chemotherapy in rats |
| title_fullStr | Assessment role of total phenols and flavonoids extracted from Pleurotus columbinus mushroom on the premature ovarian failure induced by chemotherapy in rats |
| title_full_unstemmed | Assessment role of total phenols and flavonoids extracted from Pleurotus columbinus mushroom on the premature ovarian failure induced by chemotherapy in rats |
| title_short | Assessment role of total phenols and flavonoids extracted from Pleurotus columbinus mushroom on the premature ovarian failure induced by chemotherapy in rats |
| title_sort | assessment role of total phenols and flavonoids extracted from pleurotus columbinus mushroom on the premature ovarian failure induced by chemotherapy in rats |
| topic | Cyclophosphamide Mushroom Flavonoids Total phenols Natural antioxidant |
| url | https://doi.org/10.1186/s43141-021-00278-0 |
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