Physiological Potential of Seeds of <i>Handroanthus spongiosus</i> (Rizzini) S. Grose (Bignoniaceae) Determined by the Tetrazolium Test
Tetrazolium test (TZT) can quickly evaluate in detail the viability and vigor of seeds. This study aimed to determine the optimal conditions for conducting the TZT on seeds of <i>Handroanthus spongiosus</i>. For this purpose, seeds from three lots were pre-soaked in water for 16 h, follo...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Seeds |
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| Online Access: | https://www.mdpi.com/2674-1024/2/2/16 |
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| author | Jailton de Jesus Silva Raquel Araujo Gomes Maria Aparecida Rodrigues Ferreira Claudineia Regina Pelacani Bárbara França Dantas |
| author_facet | Jailton de Jesus Silva Raquel Araujo Gomes Maria Aparecida Rodrigues Ferreira Claudineia Regina Pelacani Bárbara França Dantas |
| author_sort | Jailton de Jesus Silva |
| collection | DOAJ |
| description | Tetrazolium test (TZT) can quickly evaluate in detail the viability and vigor of seeds. This study aimed to determine the optimal conditions for conducting the TZT on seeds of <i>Handroanthus spongiosus</i>. For this purpose, seeds from three lots were pre-soaked in water for 16 h, followed by extraction of the tegument and immersion in tetrazolium salt solutions at different concentrations (0.01–0.1%), for increasing periods (1–4 h) and at 30 °C in the dark. The experimental design was completely randomized with a 4 × 4 factorial scheme with 25 seeds per repetition. We applied generalized linear models and the Tukey test for pairwise comparisons of the means at 5% probability. The viability/vigor results were compared with data obtained from the germination test at 25 °C using a subsample of seeds from the same lots. The time (1 h to 4 h) of immersion of the seeds in tetrazolium salt solutions did not cause a clear coloration difference. The seeds subjected to all treatment concentrations for 3 h presented average viability greater than 60%, with no difference in germination percentage. The TZT at 0.01% tetrazolium salt solution for 3 h was most efficient in assessing the viability of the <i>Handroanthus spongiosus</i> seeds. |
| first_indexed | 2024-03-11T01:58:12Z |
| format | Article |
| id | doaj.art-9f5cf2a6bbc4432d9ac583202dcde274 |
| institution | Directory Open Access Journal |
| issn | 2674-1024 |
| language | English |
| last_indexed | 2024-03-11T01:58:12Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | Seeds |
| spelling | doaj.art-9f5cf2a6bbc4432d9ac583202dcde2742023-11-18T12:29:51ZengMDPI AGSeeds2674-10242023-04-012220821910.3390/seeds2020016Physiological Potential of Seeds of <i>Handroanthus spongiosus</i> (Rizzini) S. Grose (Bignoniaceae) Determined by the Tetrazolium TestJailton de Jesus Silva0Raquel Araujo Gomes1Maria Aparecida Rodrigues Ferreira2Claudineia Regina Pelacani3Bárbara França Dantas4Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Avenida Transnordestina, s/n-Novo Horizonte, Feira de Santana 44036-900, BA, BrazilDepartamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Avenida Transnordestina, s/n-Novo Horizonte, Feira de Santana 44036-900, BA, BrazilDepartamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Avenida Transnordestina, s/n-Novo Horizonte, Feira de Santana 44036-900, BA, BrazilDepartamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Avenida Transnordestina, s/n-Novo Horizonte, Feira de Santana 44036-900, BA, BrazilEmbrapa Semiárido, Rodovia BR-428, Km 152, Zona Rural, Petrolina 56302-970, PE, BrazilTetrazolium test (TZT) can quickly evaluate in detail the viability and vigor of seeds. This study aimed to determine the optimal conditions for conducting the TZT on seeds of <i>Handroanthus spongiosus</i>. For this purpose, seeds from three lots were pre-soaked in water for 16 h, followed by extraction of the tegument and immersion in tetrazolium salt solutions at different concentrations (0.01–0.1%), for increasing periods (1–4 h) and at 30 °C in the dark. The experimental design was completely randomized with a 4 × 4 factorial scheme with 25 seeds per repetition. We applied generalized linear models and the Tukey test for pairwise comparisons of the means at 5% probability. The viability/vigor results were compared with data obtained from the germination test at 25 °C using a subsample of seeds from the same lots. The time (1 h to 4 h) of immersion of the seeds in tetrazolium salt solutions did not cause a clear coloration difference. The seeds subjected to all treatment concentrations for 3 h presented average viability greater than 60%, with no difference in germination percentage. The TZT at 0.01% tetrazolium salt solution for 3 h was most efficient in assessing the viability of the <i>Handroanthus spongiosus</i> seeds.https://www.mdpi.com/2674-1024/2/2/16germinationforest seedsvigor testssete-cascas |
| spellingShingle | Jailton de Jesus Silva Raquel Araujo Gomes Maria Aparecida Rodrigues Ferreira Claudineia Regina Pelacani Bárbara França Dantas Physiological Potential of Seeds of <i>Handroanthus spongiosus</i> (Rizzini) S. Grose (Bignoniaceae) Determined by the Tetrazolium Test Seeds germination forest seeds vigor tests sete-cascas |
| title | Physiological Potential of Seeds of <i>Handroanthus spongiosus</i> (Rizzini) S. Grose (Bignoniaceae) Determined by the Tetrazolium Test |
| title_full | Physiological Potential of Seeds of <i>Handroanthus spongiosus</i> (Rizzini) S. Grose (Bignoniaceae) Determined by the Tetrazolium Test |
| title_fullStr | Physiological Potential of Seeds of <i>Handroanthus spongiosus</i> (Rizzini) S. Grose (Bignoniaceae) Determined by the Tetrazolium Test |
| title_full_unstemmed | Physiological Potential of Seeds of <i>Handroanthus spongiosus</i> (Rizzini) S. Grose (Bignoniaceae) Determined by the Tetrazolium Test |
| title_short | Physiological Potential of Seeds of <i>Handroanthus spongiosus</i> (Rizzini) S. Grose (Bignoniaceae) Determined by the Tetrazolium Test |
| title_sort | physiological potential of seeds of i handroanthus spongiosus i rizzini s grose bignoniaceae determined by the tetrazolium test |
| topic | germination forest seeds vigor tests sete-cascas |
| url | https://www.mdpi.com/2674-1024/2/2/16 |
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