Prevalence and Virulent Gene Profiles of Sorbitol Non-Fermenting Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Goats in Southern Thailand
Shiga toxin-producing <i>Escherichia coli</i> (STEC) is the pathogenic <i>E. coli</i> causing disease in humans via the consumption or handling of animal food products. The high prevalence of these organisms in ruminants has been widely reported. Among STECs, O157 is one of t...
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MDPI AG
2022-11-01
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| Series: | Tropical Medicine and Infectious Disease |
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| Online Access: | https://www.mdpi.com/2414-6366/7/11/357 |
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| author | Ratchakul Wiriyaprom Ruttayaporn Ngasaman Domechai Kaewnoi Sakaoporn Prachantasena |
| author_facet | Ratchakul Wiriyaprom Ruttayaporn Ngasaman Domechai Kaewnoi Sakaoporn Prachantasena |
| author_sort | Ratchakul Wiriyaprom |
| collection | DOAJ |
| description | Shiga toxin-producing <i>Escherichia coli</i> (STEC) is the pathogenic <i>E. coli</i> causing disease in humans via the consumption or handling of animal food products. The high prevalence of these organisms in ruminants has been widely reported. Among STECs, O157 is one of the most lethal serotypes causing serious disease in humans. The present study investigated the prevalence of sorbitol non-fermenting STECs in goats reared in the lower region of southern Thailand and described the virulent factors carried by those isolates. Sorbitol non-fermenting (SNF)-STECs were found in 57 out of 646 goats (8.82%; 95% CI 6.75% to 11.28%). Molecular identification revealed that 0.77% of SNF-STEC isolates were the O157 serotype. Shiga toxin genes (<i>stx<sub>1</sub></i> and <i>stx<sub>2</sub></i>) and other virulent genes (i.e., <i>eae</i>A, <i>ehx</i>A, and <i>saa</i>) were detected by molecular techniques. The presence of <i>stx<sub>1</sub></i> (75.44%) was significantly higher than that of <i>stx<sub>2</sub></i> (22.81%), whereas 1.75% of the total isolates carried both <i>stx<sub>1</sub></i> and <i>stx<sub>2</sub></i>. Most of the isolates carried <i>ehx</i>A for 75.44%, followed by <i>saa</i> (42.11%) and <i>eae</i>A (12.28%). In addition, 21.05% of STEC isolates did not carry any <i>eae</i>A, <i>ehx</i>A, or <i>saa</i>. The first investigation on SNF-STECs in goat was conducted in the lower region of southern Thailand. The present study revealed that goats could be one of the potential carriers of SNF-STECs in the observing area. |
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| institution | Directory Open Access Journal |
| issn | 2414-6366 |
| language | English |
| last_indexed | 2024-03-09T18:35:25Z |
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| spelling | doaj.art-9f5f5a38d686492899bfd9acb1bed2082023-11-24T07:11:37ZengMDPI AGTropical Medicine and Infectious Disease2414-63662022-11-0171135710.3390/tropicalmed7110357Prevalence and Virulent Gene Profiles of Sorbitol Non-Fermenting Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Goats in Southern ThailandRatchakul Wiriyaprom0Ruttayaporn Ngasaman1Domechai Kaewnoi2Sakaoporn Prachantasena3Faculty of Veterinary Science, Prince of Songkla University, Songkhla 90110, ThailandFaculty of Veterinary Science, Prince of Songkla University, Songkhla 90110, ThailandFaculty of Veterinary Science, Prince of Songkla University, Songkhla 90110, ThailandFaculty of Veterinary Science, Prince of Songkla University, Songkhla 90110, ThailandShiga toxin-producing <i>Escherichia coli</i> (STEC) is the pathogenic <i>E. coli</i> causing disease in humans via the consumption or handling of animal food products. The high prevalence of these organisms in ruminants has been widely reported. Among STECs, O157 is one of the most lethal serotypes causing serious disease in humans. The present study investigated the prevalence of sorbitol non-fermenting STECs in goats reared in the lower region of southern Thailand and described the virulent factors carried by those isolates. Sorbitol non-fermenting (SNF)-STECs were found in 57 out of 646 goats (8.82%; 95% CI 6.75% to 11.28%). Molecular identification revealed that 0.77% of SNF-STEC isolates were the O157 serotype. Shiga toxin genes (<i>stx<sub>1</sub></i> and <i>stx<sub>2</sub></i>) and other virulent genes (i.e., <i>eae</i>A, <i>ehx</i>A, and <i>saa</i>) were detected by molecular techniques. The presence of <i>stx<sub>1</sub></i> (75.44%) was significantly higher than that of <i>stx<sub>2</sub></i> (22.81%), whereas 1.75% of the total isolates carried both <i>stx<sub>1</sub></i> and <i>stx<sub>2</sub></i>. Most of the isolates carried <i>ehx</i>A for 75.44%, followed by <i>saa</i> (42.11%) and <i>eae</i>A (12.28%). In addition, 21.05% of STEC isolates did not carry any <i>eae</i>A, <i>ehx</i>A, or <i>saa</i>. The first investigation on SNF-STECs in goat was conducted in the lower region of southern Thailand. The present study revealed that goats could be one of the potential carriers of SNF-STECs in the observing area.https://www.mdpi.com/2414-6366/7/11/357sorbitol non-fermenting Shiga toxin-producing <i>Escherichia coli</i>goatssouthern Thailandvirulent genes |
| spellingShingle | Ratchakul Wiriyaprom Ruttayaporn Ngasaman Domechai Kaewnoi Sakaoporn Prachantasena Prevalence and Virulent Gene Profiles of Sorbitol Non-Fermenting Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Goats in Southern Thailand Tropical Medicine and Infectious Disease sorbitol non-fermenting Shiga toxin-producing <i>Escherichia coli</i> goats southern Thailand virulent genes |
| title | Prevalence and Virulent Gene Profiles of Sorbitol Non-Fermenting Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Goats in Southern Thailand |
| title_full | Prevalence and Virulent Gene Profiles of Sorbitol Non-Fermenting Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Goats in Southern Thailand |
| title_fullStr | Prevalence and Virulent Gene Profiles of Sorbitol Non-Fermenting Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Goats in Southern Thailand |
| title_full_unstemmed | Prevalence and Virulent Gene Profiles of Sorbitol Non-Fermenting Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Goats in Southern Thailand |
| title_short | Prevalence and Virulent Gene Profiles of Sorbitol Non-Fermenting Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Goats in Southern Thailand |
| title_sort | prevalence and virulent gene profiles of sorbitol non fermenting shiga toxin producing i escherichia coli i isolated from goats in southern thailand |
| topic | sorbitol non-fermenting Shiga toxin-producing <i>Escherichia coli</i> goats southern Thailand virulent genes |
| url | https://www.mdpi.com/2414-6366/7/11/357 |
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