Differentially expressed serum proteins associated with calcium regulation and hypocalcemia in dairy cows
Objective Hypocalcemia is an important metabolic disease of dairy cows during the transition period, although the effect of hypocalcemia on biological function in dairy cows remains unknown. Methods In this study, proteomic, mass spectrum, bioinformatics and western blotting were employed to identif...
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| Format: | Article |
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Asian-Australasian Association of Animal Production Societies
2017-06-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-30-6-893.pdf |
| _version_ | 1818328043265982464 |
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| author | Shi Shu Yunlong Bai Gang Wang Xinhuan Xiao Ziling Fan Jiang Zhang Chang Zhao Yang Zhao Cheng Xia Hongyou Zhang |
| author_facet | Shi Shu Yunlong Bai Gang Wang Xinhuan Xiao Ziling Fan Jiang Zhang Chang Zhao Yang Zhao Cheng Xia Hongyou Zhang |
| author_sort | Shi Shu |
| collection | DOAJ |
| description | Objective Hypocalcemia is an important metabolic disease of dairy cows during the transition period, although the effect of hypocalcemia on biological function in dairy cows remains unknown. Methods In this study, proteomic, mass spectrum, bioinformatics and western blotting were employed to identify differentially expressed proteins related to serum Ca concentration. Serum samples from dairy cows were collected at three time points: 3rd days before calving (day −3), the day of calving (day 0), and 3rd days after calving (day +3). According to the Ca concentration on day 0, a total of 27 dairy cows were assigned to one of three groups (clinical, subclinical, and healthy). Samples collected on day −3 were used for discovery of differentially expressed proteins, which were separated and identified via proteomic analysis and mass spectrometry. Bioinformatics analysis was performed to determine the function of the identified proteins (gene ontology and pathway analysis). The differentially expressed proteins were verified by western blot analysis. Results There were 57 differential spots separated and eight different proteins were identified. Vitamin D-binding protein precursor (group-specific component, GC), alpha-2-macroglobulin (A2M) protein, and apolipoprotein A-IV were related to hypocalcemia by bioinformatics analysis. Due to its specific expression (up-regulated in clinical hypocalcemia and down-regulated in subclinical hypocalcemia), A2M was selected for validation. The results were consistent with those of proteomic analysis. Conclusion A2M was as an early detection index for distinguishing clinical and subclinical hypocalcemia. The possible pathogenesis of clinical hypocalcemia caused by GC and apolipoprotein A-IV was speculated. The down-regulated expression of GC was a probable cause of the decrease in calcium concentration. |
| first_indexed | 2024-12-13T12:25:53Z |
| format | Article |
| id | doaj.art-9f6016561a4f4d318d7129f807c9bb57 |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-13T12:25:53Z |
| publishDate | 2017-06-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-9f6016561a4f4d318d7129f807c9bb572022-12-21T23:46:21ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172017-06-0130689390110.5713/ajas.16.061523659Differentially expressed serum proteins associated with calcium regulation and hypocalcemia in dairy cowsShi Shu0Yunlong Bai1Gang Wang2Xinhuan Xiao3Ziling Fan4Jiang Zhang5Chang Zhao6Yang Zhao7Cheng Xia8Hongyou Zhang9 College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaObjective Hypocalcemia is an important metabolic disease of dairy cows during the transition period, although the effect of hypocalcemia on biological function in dairy cows remains unknown. Methods In this study, proteomic, mass spectrum, bioinformatics and western blotting were employed to identify differentially expressed proteins related to serum Ca concentration. Serum samples from dairy cows were collected at three time points: 3rd days before calving (day −3), the day of calving (day 0), and 3rd days after calving (day +3). According to the Ca concentration on day 0, a total of 27 dairy cows were assigned to one of three groups (clinical, subclinical, and healthy). Samples collected on day −3 were used for discovery of differentially expressed proteins, which were separated and identified via proteomic analysis and mass spectrometry. Bioinformatics analysis was performed to determine the function of the identified proteins (gene ontology and pathway analysis). The differentially expressed proteins were verified by western blot analysis. Results There were 57 differential spots separated and eight different proteins were identified. Vitamin D-binding protein precursor (group-specific component, GC), alpha-2-macroglobulin (A2M) protein, and apolipoprotein A-IV were related to hypocalcemia by bioinformatics analysis. Due to its specific expression (up-regulated in clinical hypocalcemia and down-regulated in subclinical hypocalcemia), A2M was selected for validation. The results were consistent with those of proteomic analysis. Conclusion A2M was as an early detection index for distinguishing clinical and subclinical hypocalcemia. The possible pathogenesis of clinical hypocalcemia caused by GC and apolipoprotein A-IV was speculated. The down-regulated expression of GC was a probable cause of the decrease in calcium concentration.http://www.ajas.info/upload/pdf/ajas-30-6-893.pdfDairy CowsHypocalcemiaProteomicBioinformatics |
| spellingShingle | Shi Shu Yunlong Bai Gang Wang Xinhuan Xiao Ziling Fan Jiang Zhang Chang Zhao Yang Zhao Cheng Xia Hongyou Zhang Differentially expressed serum proteins associated with calcium regulation and hypocalcemia in dairy cows Asian-Australasian Journal of Animal Sciences Dairy Cows Hypocalcemia Proteomic Bioinformatics |
| title | Differentially expressed serum proteins associated with calcium regulation and hypocalcemia in dairy cows |
| title_full | Differentially expressed serum proteins associated with calcium regulation and hypocalcemia in dairy cows |
| title_fullStr | Differentially expressed serum proteins associated with calcium regulation and hypocalcemia in dairy cows |
| title_full_unstemmed | Differentially expressed serum proteins associated with calcium regulation and hypocalcemia in dairy cows |
| title_short | Differentially expressed serum proteins associated with calcium regulation and hypocalcemia in dairy cows |
| title_sort | differentially expressed serum proteins associated with calcium regulation and hypocalcemia in dairy cows |
| topic | Dairy Cows Hypocalcemia Proteomic Bioinformatics |
| url | http://www.ajas.info/upload/pdf/ajas-30-6-893.pdf |
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