In vitro</font></i><font size="3"> establishment and multiplication of <i>Aloe vera</i> L. shoots
The high demand of Aloe vera L. in the pharmaceutical and cosmetic industries has caused that the traditional crop is insufficient to satisfy the current market. In Cuba, this plant is used as a constituent of many pharmaceutical and cosmetic products. At present, a large part of Aloe raw material i...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidad Central Marta Abreu de Las Villas
2015-04-01
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| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/14 |
| _version_ | 1828467352194252800 |
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| author | Naivy Pérez Alonso Alina Capote Anabel Pérez Leticia Gómez Elio Jiménez |
| author_facet | Naivy Pérez Alonso Alina Capote Anabel Pérez Leticia Gómez Elio Jiménez |
| author_sort | Naivy Pérez Alonso |
| collection | DOAJ |
| description | The high demand of Aloe vera L. in the pharmaceutical and cosmetic industries has caused that the traditional crop is insufficient to satisfy the current market. In Cuba, this plant is used as a constituent of many pharmaceutical and cosmetic products. At present, a large part of Aloe raw material is imported due to the lack of sufficiently large populations to meet all the demand. In vitro propagation is one of the alternatives to increase the availability of biomass of this specie. For this reason, this research was carried out in order to study the effect of the time of collecting plant material and conditions disinfection to establishment in vitro shoots. Besides, the use of growth regulators in multiplication phase was studied. The results showed that in vitro establishment of apices with 93% microbial contamination-free explants was achieved taking plant material in the dry season and disinfecting with 2.0% sodium hypochlorite for 15 min. In the multiplication phase were obtained best results in culture medium containing the combination of 2.0 mg l-1 of 6-BAP and 0.5 mg l-1 of AIB, making a transversal cut to shoot. In these conditions, the multiplication coefficients obtained was stable during nine subcultures with maximum valour of 5.1.
Key words: cytokinins, disinfection, season, management explants |
| first_indexed | 2024-12-11T04:05:13Z |
| format | Article |
| id | doaj.art-9f603d0eb0204a439d9b5a7d3cb2727c |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-11T04:05:13Z |
| publishDate | 2015-04-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-9f603d0eb0204a439d9b5a7d3cb2727c2022-12-22T01:21:32ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472015-04-0115212In vitro</font></i><font size="3"> establishment and multiplication of <i>Aloe vera</i> L. shootsNaivy Pérez Alonso0Alina Capote1Anabel Pérez2Leticia Gómez3Elio Jiménez4Instituto de Biotecnología de las Plantas. Universidad Central Marta Abreu de Las VillasInstituto de Biotecnología de las Plantas. Universidad Central Marta Abreu de Las VillasInstituto de Biotecnología de las Plantas. Universidad Central Marta Abreu de Las VillasInstituto de Biotecnología de las Plantas. Universidad Central Marta Abreu de Las VillasTropical Research and Education Center. Institute of Food and Agricultural Sciences. University of FloridaThe high demand of Aloe vera L. in the pharmaceutical and cosmetic industries has caused that the traditional crop is insufficient to satisfy the current market. In Cuba, this plant is used as a constituent of many pharmaceutical and cosmetic products. At present, a large part of Aloe raw material is imported due to the lack of sufficiently large populations to meet all the demand. In vitro propagation is one of the alternatives to increase the availability of biomass of this specie. For this reason, this research was carried out in order to study the effect of the time of collecting plant material and conditions disinfection to establishment in vitro shoots. Besides, the use of growth regulators in multiplication phase was studied. The results showed that in vitro establishment of apices with 93% microbial contamination-free explants was achieved taking plant material in the dry season and disinfecting with 2.0% sodium hypochlorite for 15 min. In the multiplication phase were obtained best results in culture medium containing the combination of 2.0 mg l-1 of 6-BAP and 0.5 mg l-1 of AIB, making a transversal cut to shoot. In these conditions, the multiplication coefficients obtained was stable during nine subcultures with maximum valour of 5.1. Key words: cytokinins, disinfection, season, management explantshttps://revista.ibp.co.cu/index.php/BV/article/view/14 |
| spellingShingle | Naivy Pérez Alonso Alina Capote Anabel Pérez Leticia Gómez Elio Jiménez In vitro</font></i><font size="3"> establishment and multiplication of <i>Aloe vera</i> L. shoots Biotecnología Vegetal |
| title | In vitro</font></i><font size="3"> establishment and multiplication of <i>Aloe vera</i> L. shoots |
| title_full | In vitro</font></i><font size="3"> establishment and multiplication of <i>Aloe vera</i> L. shoots |
| title_fullStr | In vitro</font></i><font size="3"> establishment and multiplication of <i>Aloe vera</i> L. shoots |
| title_full_unstemmed | In vitro</font></i><font size="3"> establishment and multiplication of <i>Aloe vera</i> L. shoots |
| title_short | In vitro</font></i><font size="3"> establishment and multiplication of <i>Aloe vera</i> L. shoots |
| title_sort | in vitro font i font size 3 establishment and multiplication of i aloe vera i l shoots |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/14 |
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