Evaluation of the mutagenic effect of the iodinated contrast medium Urografina<sup>®</sup> 292 using the micronucleus test in mouse bone marrow cells
<p id="para1">Contrast media (CM) are frequently used in diagnostic radiology and in radiotherapy as a diagnostic tool and in treatment planning. Previous studies have demonstrated that these compounds induce chromosomal aberrations. This study evaluates the mutagenic effects induced...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2013-06-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000200737 |
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| author | MÔNICA B.B. BELLE DANIELA D. LEFFA DALIANE MAZZORANA VANESSA M. DE ANDRADE |
| author_facet | MÔNICA B.B. BELLE DANIELA D. LEFFA DALIANE MAZZORANA VANESSA M. DE ANDRADE |
| author_sort | MÔNICA B.B. BELLE |
| collection | DOAJ |
| description | <p id="para1">Contrast media (CM) are frequently used in diagnostic radiology and in radiotherapy as a diagnostic tool and in treatment planning. Previous studies have demonstrated that these compounds induce chromosomal aberrations. This study evaluates the mutagenic effects induced by the contrast medium Urografina<sup>®</sup> 292 (meglumine amidotrizoate and sodium-ionic dimmer) in bone marrow cells (BMC) of mice <italic>in vivo</italic>. Micronuclei assay was performed in BMC of CF-1 mice injected with CM 1.5 and 3.0 mL/kg intravenous doses and 1.0, 2.0, 3.0 mL/kg intraperitoneal doses. The animals were beheaded 24 h after treatment by cervical dislocation, and femur BMC from each animal were used in the micronucleus test. The group treated with the highest intravenous injection of Urografina<sup>®</sup> 292 (3.0 mL/kg) presented an increase in the frequency of micronucleated polychromatic erythrocytes (MNPCEs) in relation at the control group (P<0.05). The results obtained after intraperitoneal administration of CM showed that all doses (1.0 mL/kg, 2.0 mL/kg and 3.0 mL/kg) increased the frequency of MNPCEs, being significantly different from the negative control (P< 0.01). The present results suggest that iodinated contrast media Urografina<sup>®</sup> 292 may cause a significant increase of cytogenetic damage in bone marrow cells of mice.</p><br><p id="para2">Meios de contraste (MC) são freqüentemente utilizados em radiodiagnóstico e em radioterapia como uma ferramenta de diagnóstico e em planejamento do tratamento. Estudos prévios demonstraram que estes compostos induzem aberrações cromossômicas. Este estudo avaliou os efeitos mutagênicos induzidos pelo meio de contraste Urografina<sup>®</sup> 292 (Amidotrizoate de meglumina e sódio-dímero iônico) em células da medula óssea (BMC) de camundongos <italic>in vivo</italic>. Teste de micronúcleos foi realizado em BMC de camundongos CF-1, injetados com MC intravenoso nas doses 1,5 e 3,0 mL/kg e intraperitonealmente nas doses 1,0, 2,0 e 3,0 mL/kg. Os animais foram decapitados 24 horas após o tratamento por deslocamento cervical, e as BMC dos fêmures de cada animal foram utilizados no teste de micronúcleos. O grupo tratado com injeção intravenosa mais elevada de Urografina<sup>®</sup> 292 (3,0 mL/kg) apresentou um aumento na freqüência de eritrócitos policromáticos micronucleados (MNPCEs) em relação ao grupo controle (P<0,05). Os resultados obtidos após a administração intraperitoneal do MC demonstrou que todas as doses (1,0 mL/kg, 2,0 mL/kg e 3,0 mL/kg) aumentaram a freqüência de MNPCEs, sendo significativamente diferentes do controle negativo (P<0,01). Os presentes resultados sugerem que o meio de contraste iodado Urografina<sup>®</sup> 292 pode causar um aumento significativo de danos citogenéticos em células da medula óssea de camundongos.</p> |
| first_indexed | 2024-12-19T16:47:47Z |
| format | Article |
| id | doaj.art-9f61e89c07d34c6f9d6d91ccf161c860 |
| institution | Directory Open Access Journal |
| issn | 0001-3765 1678-2690 |
| language | English |
| last_indexed | 2024-12-19T16:47:47Z |
| publishDate | 2013-06-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-9f61e89c07d34c6f9d6d91ccf161c8602022-12-21T20:13:37ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências0001-37651678-26902013-06-01852737744Evaluation of the mutagenic effect of the iodinated contrast medium Urografina<sup>®</sup> 292 using the micronucleus test in mouse bone marrow cellsMÔNICA B.B. BELLEDANIELA D. LEFFADALIANE MAZZORANAVANESSA M. DE ANDRADE<p id="para1">Contrast media (CM) are frequently used in diagnostic radiology and in radiotherapy as a diagnostic tool and in treatment planning. Previous studies have demonstrated that these compounds induce chromosomal aberrations. This study evaluates the mutagenic effects induced by the contrast medium Urografina<sup>®</sup> 292 (meglumine amidotrizoate and sodium-ionic dimmer) in bone marrow cells (BMC) of mice <italic>in vivo</italic>. Micronuclei assay was performed in BMC of CF-1 mice injected with CM 1.5 and 3.0 mL/kg intravenous doses and 1.0, 2.0, 3.0 mL/kg intraperitoneal doses. The animals were beheaded 24 h after treatment by cervical dislocation, and femur BMC from each animal were used in the micronucleus test. The group treated with the highest intravenous injection of Urografina<sup>®</sup> 292 (3.0 mL/kg) presented an increase in the frequency of micronucleated polychromatic erythrocytes (MNPCEs) in relation at the control group (P<0.05). The results obtained after intraperitoneal administration of CM showed that all doses (1.0 mL/kg, 2.0 mL/kg and 3.0 mL/kg) increased the frequency of MNPCEs, being significantly different from the negative control (P< 0.01). The present results suggest that iodinated contrast media Urografina<sup>®</sup> 292 may cause a significant increase of cytogenetic damage in bone marrow cells of mice.</p><br><p id="para2">Meios de contraste (MC) são freqüentemente utilizados em radiodiagnóstico e em radioterapia como uma ferramenta de diagnóstico e em planejamento do tratamento. Estudos prévios demonstraram que estes compostos induzem aberrações cromossômicas. Este estudo avaliou os efeitos mutagênicos induzidos pelo meio de contraste Urografina<sup>®</sup> 292 (Amidotrizoate de meglumina e sódio-dímero iônico) em células da medula óssea (BMC) de camundongos <italic>in vivo</italic>. Teste de micronúcleos foi realizado em BMC de camundongos CF-1, injetados com MC intravenoso nas doses 1,5 e 3,0 mL/kg e intraperitonealmente nas doses 1,0, 2,0 e 3,0 mL/kg. Os animais foram decapitados 24 horas após o tratamento por deslocamento cervical, e as BMC dos fêmures de cada animal foram utilizados no teste de micronúcleos. O grupo tratado com injeção intravenosa mais elevada de Urografina<sup>®</sup> 292 (3,0 mL/kg) apresentou um aumento na freqüência de eritrócitos policromáticos micronucleados (MNPCEs) em relação ao grupo controle (P<0,05). Os resultados obtidos após a administração intraperitoneal do MC demonstrou que todas as doses (1,0 mL/kg, 2,0 mL/kg e 3,0 mL/kg) aumentaram a freqüência de MNPCEs, sendo significativamente diferentes do controle negativo (P<0,01). Os presentes resultados sugerem que o meio de contraste iodado Urografina<sup>®</sup> 292 pode causar um aumento significativo de danos citogenéticos em células da medula óssea de camundongos.</p>http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000200737cromossomosmeio de contrastemicronúcleosUrografina<sup>®</sup> 292chromosomescontrast mediummicronucleiUrografina<sup>®</sup> 292 |
| spellingShingle | MÔNICA B.B. BELLE DANIELA D. LEFFA DALIANE MAZZORANA VANESSA M. DE ANDRADE Evaluation of the mutagenic effect of the iodinated contrast medium Urografina<sup>®</sup> 292 using the micronucleus test in mouse bone marrow cells Anais da Academia Brasileira de Ciências cromossomos meio de contraste micronúcleos Urografina<sup>®</sup> 292 chromosomes contrast medium micronuclei Urografina<sup>®</sup> 292 |
| title | Evaluation of the mutagenic effect of the iodinated contrast medium Urografina<sup>®</sup> 292 using the micronucleus test in mouse bone marrow cells |
| title_full | Evaluation of the mutagenic effect of the iodinated contrast medium Urografina<sup>®</sup> 292 using the micronucleus test in mouse bone marrow cells |
| title_fullStr | Evaluation of the mutagenic effect of the iodinated contrast medium Urografina<sup>®</sup> 292 using the micronucleus test in mouse bone marrow cells |
| title_full_unstemmed | Evaluation of the mutagenic effect of the iodinated contrast medium Urografina<sup>®</sup> 292 using the micronucleus test in mouse bone marrow cells |
| title_short | Evaluation of the mutagenic effect of the iodinated contrast medium Urografina<sup>®</sup> 292 using the micronucleus test in mouse bone marrow cells |
| title_sort | evaluation of the mutagenic effect of the iodinated contrast medium urografina sup 174 sup 292 using the micronucleus test in mouse bone marrow cells |
| topic | cromossomos meio de contraste micronúcleos Urografina<sup>®</sup> 292 chromosomes contrast medium micronuclei Urografina<sup>®</sup> 292 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000200737 |
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