Viscoelastic Properties of ECM-Rich Embryonic Microenvironments
The material properties of tissues and their mechanical state is an important factor in development, disease, regenerative medicine and tissue engineering. Here we describe a microrheological measurement technique utilizing aggregates of microinjected ferromagnetic nickel particles to probe the visc...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-08-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2020.00674/full |
| _version_ | 1818242835836567552 |
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| author | Zsuzsa Akos Dona Greta Isai Sheeja Rajasingh Edina Kosa Saba Ghazvini Prajnaparamita Dhar Andras Czirok Andras Czirok |
| author_facet | Zsuzsa Akos Dona Greta Isai Sheeja Rajasingh Edina Kosa Saba Ghazvini Prajnaparamita Dhar Andras Czirok Andras Czirok |
| author_sort | Zsuzsa Akos |
| collection | DOAJ |
| description | The material properties of tissues and their mechanical state is an important factor in development, disease, regenerative medicine and tissue engineering. Here we describe a microrheological measurement technique utilizing aggregates of microinjected ferromagnetic nickel particles to probe the viscoelastic properties of embryonic tissues. Quail embryos were cultured in a plastic incubator chamber located at the center of two pairs of crossed electromagnets. We found a pronounced viscoelastic behavior within the ECM-rich region separating the mesoderm and endoderm in Hamburger Hamilton stage 10 quail embryos, consistent with a Zener (standard generalized solid) model. The viscoelastic response is about 45% of the total response, with a characteristic relaxation time of 1.3 s. |
| first_indexed | 2024-12-12T13:51:33Z |
| format | Article |
| id | doaj.art-9f61fd76afeb4cc4a70401b0896757f5 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-12T13:51:33Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-9f61fd76afeb4cc4a70401b0896757f52022-12-22T00:22:34ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-08-01810.3389/fcell.2020.00674512574Viscoelastic Properties of ECM-Rich Embryonic MicroenvironmentsZsuzsa Akos0Dona Greta Isai1Sheeja Rajasingh2Edina Kosa3Saba Ghazvini4Prajnaparamita Dhar5Andras Czirok6Andras Czirok7Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, United StatesDepartment of Anatomy & Cell Biology, University of Kansas Medical Center, Kansas City, KS, United StatesDepartment of Bioscience Research, University of Tennessee Health Science Center, Memphis, TN, United StatesDepartment of Research, Kansas City University of Medicine and Biosciences, Kansas City, MO, United StatesChemical & Petroleum Engineering, The University of Kansas, Lawrence, KS, United StatesChemical & Petroleum Engineering, The University of Kansas, Lawrence, KS, United StatesDepartment of Anatomy & Cell Biology, University of Kansas Medical Center, Kansas City, KS, United StatesDepartment of Biological Physics, Eotvos University, Budapest, HungaryThe material properties of tissues and their mechanical state is an important factor in development, disease, regenerative medicine and tissue engineering. Here we describe a microrheological measurement technique utilizing aggregates of microinjected ferromagnetic nickel particles to probe the viscoelastic properties of embryonic tissues. Quail embryos were cultured in a plastic incubator chamber located at the center of two pairs of crossed electromagnets. We found a pronounced viscoelastic behavior within the ECM-rich region separating the mesoderm and endoderm in Hamburger Hamilton stage 10 quail embryos, consistent with a Zener (standard generalized solid) model. The viscoelastic response is about 45% of the total response, with a characteristic relaxation time of 1.3 s.https://www.frontiersin.org/article/10.3389/fcell.2020.00674/fullECMquail embryomicrorheologyelasticityYoung's modulusZener solid |
| spellingShingle | Zsuzsa Akos Dona Greta Isai Sheeja Rajasingh Edina Kosa Saba Ghazvini Prajnaparamita Dhar Andras Czirok Andras Czirok Viscoelastic Properties of ECM-Rich Embryonic Microenvironments Frontiers in Cell and Developmental Biology ECM quail embryo microrheology elasticity Young's modulus Zener solid |
| title | Viscoelastic Properties of ECM-Rich Embryonic Microenvironments |
| title_full | Viscoelastic Properties of ECM-Rich Embryonic Microenvironments |
| title_fullStr | Viscoelastic Properties of ECM-Rich Embryonic Microenvironments |
| title_full_unstemmed | Viscoelastic Properties of ECM-Rich Embryonic Microenvironments |
| title_short | Viscoelastic Properties of ECM-Rich Embryonic Microenvironments |
| title_sort | viscoelastic properties of ecm rich embryonic microenvironments |
| topic | ECM quail embryo microrheology elasticity Young's modulus Zener solid |
| url | https://www.frontiersin.org/article/10.3389/fcell.2020.00674/full |
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