Analysis of whiB7 in Mycobacterium tuberculosis reveals novel AT-hook deletion mutations
Abstract Mutations in whiB7 have been associated with both hypersusceptibility and resistance to various antibiotics in Mycobacterium tuberculosis (Mtb). Unlocking the secrets of antibiotic resistance in the bacterium, we examined mutations in the coding sequences of whiB7 of over 40,000 diverse Mtb...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-40152-2 |
| _version_ | 1797453292232507392 |
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| author | Olabisi Flora Davies-Bolorunduro Bharkbhoom Jaemsai Wuthiwat Ruangchai Worakorn Phumiphanjarphak Pakorn Aiewsakun Prasit Palittapongarnpim |
| author_facet | Olabisi Flora Davies-Bolorunduro Bharkbhoom Jaemsai Wuthiwat Ruangchai Worakorn Phumiphanjarphak Pakorn Aiewsakun Prasit Palittapongarnpim |
| author_sort | Olabisi Flora Davies-Bolorunduro |
| collection | DOAJ |
| description | Abstract Mutations in whiB7 have been associated with both hypersusceptibility and resistance to various antibiotics in Mycobacterium tuberculosis (Mtb). Unlocking the secrets of antibiotic resistance in the bacterium, we examined mutations in the coding sequences of whiB7 of over 40,000 diverse Mtb isolates. Our results unveil the dominant c.191delG (Gly64delG) mutation, present in all members of the lineage L1.2.2 and its impact on WhiB7's conserved GVWGG-motif, causing conformational changes and deletion of the C-terminal AT-hook. Excitingly, we discovered six unique mutations associated with partial or total deletion of the AT-hook, specific to certain sublineages. Our findings suggest the selective pressures driving these mutations, underlining the potential of genomics to advance our understanding of Mtb's antibiotic resistance. As tuberculosis remains a global health threat, our study offers valuable insights into the diverse nature and functional consequences of whiB7 mutations, paving the way for the development of novel therapeutic interventions. |
| first_indexed | 2024-03-09T15:20:43Z |
| format | Article |
| id | doaj.art-9f63c5ce20d74ca1adefa8ae1bc94bd6 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:20:43Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9f63c5ce20d74ca1adefa8ae1bc94bd62023-11-26T12:49:46ZengNature PortfolioScientific Reports2045-23222023-08-011311910.1038/s41598-023-40152-2Analysis of whiB7 in Mycobacterium tuberculosis reveals novel AT-hook deletion mutationsOlabisi Flora Davies-Bolorunduro0Bharkbhoom Jaemsai1Wuthiwat Ruangchai2Worakorn Phumiphanjarphak3Pakorn Aiewsakun4Prasit Palittapongarnpim5Pornchai Matangkasombut Center for Microbial Genomics, Department of Microbiology, Faculty of Science, Mahidol UniversityDepartment of Microbiology, Faculty of Science, Mahidol UniversityPornchai Matangkasombut Center for Microbial Genomics, Department of Microbiology, Faculty of Science, Mahidol UniversityDepartment of Microbiology, Faculty of Science, Mahidol UniversityPornchai Matangkasombut Center for Microbial Genomics, Department of Microbiology, Faculty of Science, Mahidol UniversityPornchai Matangkasombut Center for Microbial Genomics, Department of Microbiology, Faculty of Science, Mahidol UniversityAbstract Mutations in whiB7 have been associated with both hypersusceptibility and resistance to various antibiotics in Mycobacterium tuberculosis (Mtb). Unlocking the secrets of antibiotic resistance in the bacterium, we examined mutations in the coding sequences of whiB7 of over 40,000 diverse Mtb isolates. Our results unveil the dominant c.191delG (Gly64delG) mutation, present in all members of the lineage L1.2.2 and its impact on WhiB7's conserved GVWGG-motif, causing conformational changes and deletion of the C-terminal AT-hook. Excitingly, we discovered six unique mutations associated with partial or total deletion of the AT-hook, specific to certain sublineages. Our findings suggest the selective pressures driving these mutations, underlining the potential of genomics to advance our understanding of Mtb's antibiotic resistance. As tuberculosis remains a global health threat, our study offers valuable insights into the diverse nature and functional consequences of whiB7 mutations, paving the way for the development of novel therapeutic interventions.https://doi.org/10.1038/s41598-023-40152-2 |
| spellingShingle | Olabisi Flora Davies-Bolorunduro Bharkbhoom Jaemsai Wuthiwat Ruangchai Worakorn Phumiphanjarphak Pakorn Aiewsakun Prasit Palittapongarnpim Analysis of whiB7 in Mycobacterium tuberculosis reveals novel AT-hook deletion mutations Scientific Reports |
| title | Analysis of whiB7 in Mycobacterium tuberculosis reveals novel AT-hook deletion mutations |
| title_full | Analysis of whiB7 in Mycobacterium tuberculosis reveals novel AT-hook deletion mutations |
| title_fullStr | Analysis of whiB7 in Mycobacterium tuberculosis reveals novel AT-hook deletion mutations |
| title_full_unstemmed | Analysis of whiB7 in Mycobacterium tuberculosis reveals novel AT-hook deletion mutations |
| title_short | Analysis of whiB7 in Mycobacterium tuberculosis reveals novel AT-hook deletion mutations |
| title_sort | analysis of whib7 in mycobacterium tuberculosis reveals novel at hook deletion mutations |
| url | https://doi.org/10.1038/s41598-023-40152-2 |
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