Co-location of QTL for Sclerotinia stem rot resistance and flowering time in Brassica napus
Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum (Lib.) de Bary is one of the most devastating diseases of Brassica napus worldwide. Both SSR resistance and flowering time (FT) adaptation are major breeding goals in B. napus. However, early maturing rapeseed varieties, which are importa...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2019-04-01
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| Series: | Crop Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514119300297 |
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| author | Jian Wu Peipei Chen Qing Zhao Guangqin Cai Yue Hu Yang Xiang Qingyong Yang Youping Wang Yongming Zhou |
| author_facet | Jian Wu Peipei Chen Qing Zhao Guangqin Cai Yue Hu Yang Xiang Qingyong Yang Youping Wang Yongming Zhou |
| author_sort | Jian Wu |
| collection | DOAJ |
| description | Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum (Lib.) de Bary is one of the most devastating diseases of Brassica napus worldwide. Both SSR resistance and flowering time (FT) adaptation are major breeding goals in B. napus. However, early maturing rapeseed varieties, which are important for rice-rapeseed rotation in China, are often highly susceptible to SSR. Here, we found that SSR resistance was significantly negatively correlated with FT in a natural population containing 521 rapeseed inbred lines and a double haploid (DH) population with 150 individual lines, both of which had great variation in FT. Four chromosomal regions on A2, A6, C2, and C8 affecting both SSR resistance and FT were identified using quantitative trait loci (QTL) mapping after constructing a high-density genetic map based on single nucleotide polymorphism markers in the DH population. Furthermore, we aligned QTL for the two traits identified in the present and previous studies to the B. napus reference genome, and identified four colocalized QTL hotspots of SSR resistance and FT on A2 (0–7.7 Mb), A3 (0.8–7.5 Mb), C2 (0–15.2 Mb), and C6 (20.2–36.6 Mb). Our results revealed a genetic link between SSR resistance and FT in B. napus, which should facilitate the development of effective strategies in both early maturing and SSR resistance breeding and in map-based cloning of SSR resistance QTL. Keywords: Sclerotinia stem rot, Brassica napus, QTL mapping, Flowering time, SNP array |
| first_indexed | 2024-12-14T21:14:58Z |
| format | Article |
| id | doaj.art-9f649be43743470ea870fab830eb4506 |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
| language | English |
| last_indexed | 2024-12-14T21:14:58Z |
| publishDate | 2019-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj.art-9f649be43743470ea870fab830eb45062022-12-21T22:47:06ZengKeAi Communications Co., Ltd.Crop Journal2214-51412019-04-0172227237Co-location of QTL for Sclerotinia stem rot resistance and flowering time in Brassica napusJian Wu0Peipei Chen1Qing Zhao2Guangqin Cai3Yue Hu4Yang Xiang5Qingyong Yang6Youping Wang7Yongming Zhou8Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, Jiangsu, China; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, ChinaJiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, Jiangsu, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, ChinaAgricultural Bioinformatics Key Laboratory of Hubei Province, College of Informatics, Huazhong Agricultural University, Wuhan 430070, Hubei, ChinaGuizhou Rapeseed Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550008, Guizhou, ChinaAgricultural Bioinformatics Key Laboratory of Hubei Province, College of Informatics, Huazhong Agricultural University, Wuhan 430070, Hubei, ChinaJiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, Jiangsu, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China; Corresponding author.Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum (Lib.) de Bary is one of the most devastating diseases of Brassica napus worldwide. Both SSR resistance and flowering time (FT) adaptation are major breeding goals in B. napus. However, early maturing rapeseed varieties, which are important for rice-rapeseed rotation in China, are often highly susceptible to SSR. Here, we found that SSR resistance was significantly negatively correlated with FT in a natural population containing 521 rapeseed inbred lines and a double haploid (DH) population with 150 individual lines, both of which had great variation in FT. Four chromosomal regions on A2, A6, C2, and C8 affecting both SSR resistance and FT were identified using quantitative trait loci (QTL) mapping after constructing a high-density genetic map based on single nucleotide polymorphism markers in the DH population. Furthermore, we aligned QTL for the two traits identified in the present and previous studies to the B. napus reference genome, and identified four colocalized QTL hotspots of SSR resistance and FT on A2 (0–7.7 Mb), A3 (0.8–7.5 Mb), C2 (0–15.2 Mb), and C6 (20.2–36.6 Mb). Our results revealed a genetic link between SSR resistance and FT in B. napus, which should facilitate the development of effective strategies in both early maturing and SSR resistance breeding and in map-based cloning of SSR resistance QTL. Keywords: Sclerotinia stem rot, Brassica napus, QTL mapping, Flowering time, SNP arrayhttp://www.sciencedirect.com/science/article/pii/S2214514119300297 |
| spellingShingle | Jian Wu Peipei Chen Qing Zhao Guangqin Cai Yue Hu Yang Xiang Qingyong Yang Youping Wang Yongming Zhou Co-location of QTL for Sclerotinia stem rot resistance and flowering time in Brassica napus Crop Journal |
| title | Co-location of QTL for Sclerotinia stem rot resistance and flowering time in Brassica napus |
| title_full | Co-location of QTL for Sclerotinia stem rot resistance and flowering time in Brassica napus |
| title_fullStr | Co-location of QTL for Sclerotinia stem rot resistance and flowering time in Brassica napus |
| title_full_unstemmed | Co-location of QTL for Sclerotinia stem rot resistance and flowering time in Brassica napus |
| title_short | Co-location of QTL for Sclerotinia stem rot resistance and flowering time in Brassica napus |
| title_sort | co location of qtl for sclerotinia stem rot resistance and flowering time in brassica napus |
| url | http://www.sciencedirect.com/science/article/pii/S2214514119300297 |
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