In vitro study of the tocolytic effect of oroxylin A from <it>Scutellaria baicalensis </it>root
<p>Abstract</p> <p>Scutellariae Radix is one of the well-known tocolytic Chinese herbs. Oroxylin A is isolated from the root of <it>Scutellaria baicalensis</it>. The main syndrome of preterm birth is caused by uterus contractions from excitatory factors. Administration...
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| Format: | Article |
| Language: | English |
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BMC
2009-03-01
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| Series: | Journal of Biomedical Science |
| Online Access: | http://www.jbiomedsci.com/content/16/1/27 |
| _version_ | 1818136860580380672 |
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| author | Yang Ling-Ling Hsu Chun-Sen Shih Huey-Chuan |
| author_facet | Yang Ling-Ling Hsu Chun-Sen Shih Huey-Chuan |
| author_sort | Yang Ling-Ling |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Scutellariae Radix is one of the well-known tocolytic Chinese herbs. Oroxylin A is isolated from the root of <it>Scutellaria baicalensis</it>. The main syndrome of preterm birth is caused by uterus contractions from excitatory factors. Administration of tocolytic agents is a strategy to prevent the occurrence of preterm births. The aim of this study was to investigate the effects of oroxylin A on contractions of uterine strips isolated from non-pregnant female Wistar rats (250~350 g). Contractions of the uterus were induced with acetylcholine (Ach) (1 μM), PGF<sub>2α </sub>(0.1 μM), oxytocin (10<sup>-3 </sup>U/ml), KCl (56.3 mM), tetraethylammonium (TEA; 1 and 10 mM), 4-aminopyridine (4-AP; 5 mM), glipizide (30 μM), a nitric oxide synthase (NOS) inhibitor (LNNA; 10<sup>-3</sup>M), a β-receptor blocker (propranolol; 10 μM), and a cyclooxygenase inhibitor (indomethacin; 60 μM). The inhibitory effects of the amplitude and frequency of spontaneous contractions by oroxylin A were antagonized with Ach (IC<sub>50 </sub>22.85 μM), PGF<sub>2α </sub>(IC<sub>50</sub>27.28 μM), oxytocin (IC<sub>50 </sub>12.34 μM), TEA; 1 and 10 mM (IC<sub>50 </sub>52.73 and 76.43 μM), 4-AP (IC<sub>50 </sub>67.16 μM), and glipizide (IC<sub>50</sub>27.53 μM), but oroxylin A was not influenced by Ca<sup>2+</sup>-free medium, LNNA, propranolol, or indomethacin. Otherwise, oroxylin A-mediated relaxation of the rat uterus might occur through opening of uterine calcium-dependent potassium channels or adenosine triphosphate potassium channel activation. This suggests that oroxylin A is the tocolytic principle constituent of <it>Scutellariae Radix</it>, and oroxylin A may provide a lead compound for new tocolytic drug development in the future.</p> |
| first_indexed | 2024-12-11T09:47:07Z |
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| id | doaj.art-9f6517dab77a4c01b2f1cab3ea01edc2 |
| institution | Directory Open Access Journal |
| issn | 1021-7770 1423-0127 |
| language | English |
| last_indexed | 2024-12-11T09:47:07Z |
| publishDate | 2009-03-01 |
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| series | Journal of Biomedical Science |
| spelling | doaj.art-9f6517dab77a4c01b2f1cab3ea01edc22022-12-22T01:12:32ZengBMCJournal of Biomedical Science1021-77701423-01272009-03-011612710.1186/1423-0127-16-27In vitro study of the tocolytic effect of oroxylin A from <it>Scutellaria baicalensis </it>rootYang Ling-LingHsu Chun-SenShih Huey-Chuan<p>Abstract</p> <p>Scutellariae Radix is one of the well-known tocolytic Chinese herbs. Oroxylin A is isolated from the root of <it>Scutellaria baicalensis</it>. The main syndrome of preterm birth is caused by uterus contractions from excitatory factors. Administration of tocolytic agents is a strategy to prevent the occurrence of preterm births. The aim of this study was to investigate the effects of oroxylin A on contractions of uterine strips isolated from non-pregnant female Wistar rats (250~350 g). Contractions of the uterus were induced with acetylcholine (Ach) (1 μM), PGF<sub>2α </sub>(0.1 μM), oxytocin (10<sup>-3 </sup>U/ml), KCl (56.3 mM), tetraethylammonium (TEA; 1 and 10 mM), 4-aminopyridine (4-AP; 5 mM), glipizide (30 μM), a nitric oxide synthase (NOS) inhibitor (LNNA; 10<sup>-3</sup>M), a β-receptor blocker (propranolol; 10 μM), and a cyclooxygenase inhibitor (indomethacin; 60 μM). The inhibitory effects of the amplitude and frequency of spontaneous contractions by oroxylin A were antagonized with Ach (IC<sub>50 </sub>22.85 μM), PGF<sub>2α </sub>(IC<sub>50</sub>27.28 μM), oxytocin (IC<sub>50 </sub>12.34 μM), TEA; 1 and 10 mM (IC<sub>50 </sub>52.73 and 76.43 μM), 4-AP (IC<sub>50 </sub>67.16 μM), and glipizide (IC<sub>50</sub>27.53 μM), but oroxylin A was not influenced by Ca<sup>2+</sup>-free medium, LNNA, propranolol, or indomethacin. Otherwise, oroxylin A-mediated relaxation of the rat uterus might occur through opening of uterine calcium-dependent potassium channels or adenosine triphosphate potassium channel activation. This suggests that oroxylin A is the tocolytic principle constituent of <it>Scutellariae Radix</it>, and oroxylin A may provide a lead compound for new tocolytic drug development in the future.</p>http://www.jbiomedsci.com/content/16/1/27 |
| spellingShingle | Yang Ling-Ling Hsu Chun-Sen Shih Huey-Chuan In vitro study of the tocolytic effect of oroxylin A from <it>Scutellaria baicalensis </it>root Journal of Biomedical Science |
| title | In vitro study of the tocolytic effect of oroxylin A from <it>Scutellaria baicalensis </it>root |
| title_full | In vitro study of the tocolytic effect of oroxylin A from <it>Scutellaria baicalensis </it>root |
| title_fullStr | In vitro study of the tocolytic effect of oroxylin A from <it>Scutellaria baicalensis </it>root |
| title_full_unstemmed | In vitro study of the tocolytic effect of oroxylin A from <it>Scutellaria baicalensis </it>root |
| title_short | In vitro study of the tocolytic effect of oroxylin A from <it>Scutellaria baicalensis </it>root |
| title_sort | in vitro study of the tocolytic effect of oroxylin a from it scutellaria baicalensis it root |
| url | http://www.jbiomedsci.com/content/16/1/27 |
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