The antihyperglycemic effect of pulsed electric field-extracted polysaccharide of Kaempferia elegans officinale on streptozotocin induced diabetic mice
Kaempferia elegans polysaccharide (KEP) was extracted using a high-voltage pulsed electric field-assisted hot water method. Its physicochemical properties, in vitro activity and hypoglycemic effect was investigated. Experiments were undertaken with diabetic mice models and the potential mechanism of...
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1053811/full |
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| author | Huan-Qing Lei Dong-Mei Li Meng-Wai Woo Xin-An Zeng Xin-An Zeng Zhong Han Zhong Han Zhong Han Zhong Han Ruo-Yong Wang |
| author_facet | Huan-Qing Lei Dong-Mei Li Meng-Wai Woo Xin-An Zeng Xin-An Zeng Zhong Han Zhong Han Zhong Han Zhong Han Ruo-Yong Wang |
| author_sort | Huan-Qing Lei |
| collection | DOAJ |
| description | Kaempferia elegans polysaccharide (KEP) was extracted using a high-voltage pulsed electric field-assisted hot water method. Its physicochemical properties, in vitro activity and hypoglycemic effect was investigated. Experiments were undertaken with diabetic mice models and the potential mechanism of KEP to improve blood glucose levels was unveiled through measurements of relevant indicators in the serum and liver of the mice. Results showed that KEP is mainly composed of glucose, rhamnose, arabinose, and galactose. It has certain DPPH and ABTS free radical scavenging ability and good α-glucosidase inhibitory ability, indicating that KEP has the potential to improve blood glucose levels in diabetes patients. The experimental results of KEP treatment on mice showed that KEP could control the continuous increase of fasting blood glucose levels. The potential mechanisms behind this blood glucose level control composes of (1) increasing the glucokinase and C peptide levels and decreasing Glucose-6-phosphatase content for improving key enzyme activity in the glucose metabolism pathway. This promotes the consumption of blood glucose during glycolysis, thereby inhibiting the production of endogenous glucose in gluconeogenesis pathway; (2) reducing triglyceride, total cholesterol, low density lipoprotein cholesterol, and increasing high density lipoprotein cholesterol content, for regulating blood lipid indicators to normal levels; and (3) by improving the activities of catalase, glutathione peroxidase, and antioxidant enzymes superoxide dismutase for further improving the antioxidant defense system in the body to reduce blood glucose. |
| first_indexed | 2024-04-13T10:01:54Z |
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| id | doaj.art-9f682fe0a3f94a5286a762c1af50ef6b |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-13T10:01:54Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-9f682fe0a3f94a5286a762c1af50ef6b2022-12-22T02:51:13ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-12-01910.3389/fnut.2022.10538111053811The antihyperglycemic effect of pulsed electric field-extracted polysaccharide of Kaempferia elegans officinale on streptozotocin induced diabetic miceHuan-Qing Lei0Dong-Mei Li1Meng-Wai Woo2Xin-An Zeng3Xin-An Zeng4Zhong Han5Zhong Han6Zhong Han7Zhong Han8Ruo-Yong Wang9School of Food Science and Engineering, South China University of Technology, Guangzhou, ChinaGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaDepartment of Chemical and Materials Engineering, University of Auckland, Auckland, New ZealandDepartment of Food Science, Foshan University, Foshan, Guangdong, ChinaPreparatory Office of Yangjiang Applied Undergraduate College, Yangjiang, ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou, ChinaPreparatory Office of Yangjiang Applied Undergraduate College, Yangjiang, ChinaOverseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou, ChinaGuangdong Provincial Key Laboratory of Intelligent Food Manufacturing, Foshan University, Foshan, ChinaAir Force Medical Center People’s Liberation Army, Beijing, ChinaKaempferia elegans polysaccharide (KEP) was extracted using a high-voltage pulsed electric field-assisted hot water method. Its physicochemical properties, in vitro activity and hypoglycemic effect was investigated. Experiments were undertaken with diabetic mice models and the potential mechanism of KEP to improve blood glucose levels was unveiled through measurements of relevant indicators in the serum and liver of the mice. Results showed that KEP is mainly composed of glucose, rhamnose, arabinose, and galactose. It has certain DPPH and ABTS free radical scavenging ability and good α-glucosidase inhibitory ability, indicating that KEP has the potential to improve blood glucose levels in diabetes patients. The experimental results of KEP treatment on mice showed that KEP could control the continuous increase of fasting blood glucose levels. The potential mechanisms behind this blood glucose level control composes of (1) increasing the glucokinase and C peptide levels and decreasing Glucose-6-phosphatase content for improving key enzyme activity in the glucose metabolism pathway. This promotes the consumption of blood glucose during glycolysis, thereby inhibiting the production of endogenous glucose in gluconeogenesis pathway; (2) reducing triglyceride, total cholesterol, low density lipoprotein cholesterol, and increasing high density lipoprotein cholesterol content, for regulating blood lipid indicators to normal levels; and (3) by improving the activities of catalase, glutathione peroxidase, and antioxidant enzymes superoxide dismutase for further improving the antioxidant defense system in the body to reduce blood glucose.https://www.frontiersin.org/articles/10.3389/fnut.2022.1053811/fullKaempferia elegans polysaccharidestreptozotocin induced diabetic micegluconeogenesisfasting blood glucoseenzyme activity |
| spellingShingle | Huan-Qing Lei Dong-Mei Li Meng-Wai Woo Xin-An Zeng Xin-An Zeng Zhong Han Zhong Han Zhong Han Zhong Han Ruo-Yong Wang The antihyperglycemic effect of pulsed electric field-extracted polysaccharide of Kaempferia elegans officinale on streptozotocin induced diabetic mice Frontiers in Nutrition Kaempferia elegans polysaccharide streptozotocin induced diabetic mice gluconeogenesis fasting blood glucose enzyme activity |
| title | The antihyperglycemic effect of pulsed electric field-extracted polysaccharide of Kaempferia elegans officinale on streptozotocin induced diabetic mice |
| title_full | The antihyperglycemic effect of pulsed electric field-extracted polysaccharide of Kaempferia elegans officinale on streptozotocin induced diabetic mice |
| title_fullStr | The antihyperglycemic effect of pulsed electric field-extracted polysaccharide of Kaempferia elegans officinale on streptozotocin induced diabetic mice |
| title_full_unstemmed | The antihyperglycemic effect of pulsed electric field-extracted polysaccharide of Kaempferia elegans officinale on streptozotocin induced diabetic mice |
| title_short | The antihyperglycemic effect of pulsed electric field-extracted polysaccharide of Kaempferia elegans officinale on streptozotocin induced diabetic mice |
| title_sort | antihyperglycemic effect of pulsed electric field extracted polysaccharide of kaempferia elegans officinale on streptozotocin induced diabetic mice |
| topic | Kaempferia elegans polysaccharide streptozotocin induced diabetic mice gluconeogenesis fasting blood glucose enzyme activity |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1053811/full |
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