Using Sentinel Plots to Monitor for Changes in Thrips Susceptibility to MON 88702 Cotton Containing the Cry51Aa2.834_16 <i>Bt</i> Protein
Transgenic <i>Bt</i> crops are important tools for growers to manage insect pests, but their durability is threatened by the evolution of insect resistance. Implementing a resistance monitoring program is essential to detect and mitigate resistance. For non-high-dose <i>Bt</i>...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/14/6/497 |
| _version_ | 1827737045973336064 |
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| author | Ashley D. Yates-Stewart Benjamin T. Yorke Alan Willse Jennifer Fridley Graham P. Head |
| author_facet | Ashley D. Yates-Stewart Benjamin T. Yorke Alan Willse Jennifer Fridley Graham P. Head |
| author_sort | Ashley D. Yates-Stewart |
| collection | DOAJ |
| description | Transgenic <i>Bt</i> crops are important tools for growers to manage insect pests, but their durability is threatened by the evolution of insect resistance. Implementing a resistance monitoring program is essential to detect and mitigate resistance. For non-high-dose <i>Bt</i> crops, resistance monitoring is challenging, because insect control is not complete, so targeted insects and insect damage will be present even without resistance. Given these challenges, sentinel plots have been used to monitor for insect resistance to non-high-dose crops by assessing changes in the efficacy of a <i>Bt</i> crop over time relative to a non-<i>Bt</i> control. We optimized a sentinel plot resistance monitoring approach for MON 88702 ThryvOn™ cotton, a new non-high-dose <i>Bt</i> product targeting two sucking pest taxa—<i>Lygus</i> (<i>L. lineolaris</i> and <i>L. hesperus</i>) and thrips (<i>Frankliniella fusca</i> and <i>F. occidentalis</i>)—and report here on the thrips monitoring methods and results. Quantifying thrips immatures was the best metric to characterize the impact of the trait, with at least a 40–60% average reduction of thrips immatures on ThryvOn relative to the control cotton at all sites with higher thrips densities. These data can be used within a ThryvOn resistance monitoring program and represent a case study for establishing a resistance monitoring approach for a non-high-dose trait product. |
| first_indexed | 2024-03-11T02:19:35Z |
| format | Article |
| id | doaj.art-9f6876133bd84bfdb5b367acd7aafca2 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T02:19:35Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-9f6876133bd84bfdb5b367acd7aafca22023-11-18T10:55:39ZengMDPI AGInsects2075-44502023-05-0114649710.3390/insects14060497Using Sentinel Plots to Monitor for Changes in Thrips Susceptibility to MON 88702 Cotton Containing the Cry51Aa2.834_16 <i>Bt</i> ProteinAshley D. Yates-Stewart0Benjamin T. Yorke1Alan Willse2Jennifer Fridley3Graham P. Head4Bayer Crop Science, Chesterfield, MO 63017, USABayer Crop Science, Chesterfield, MO 63017, USABayer Crop Science, Chesterfield, MO 63017, USABayer Crop Science, Chesterfield, MO 63017, USABayer Crop Science, Chesterfield, MO 63017, USATransgenic <i>Bt</i> crops are important tools for growers to manage insect pests, but their durability is threatened by the evolution of insect resistance. Implementing a resistance monitoring program is essential to detect and mitigate resistance. For non-high-dose <i>Bt</i> crops, resistance monitoring is challenging, because insect control is not complete, so targeted insects and insect damage will be present even without resistance. Given these challenges, sentinel plots have been used to monitor for insect resistance to non-high-dose crops by assessing changes in the efficacy of a <i>Bt</i> crop over time relative to a non-<i>Bt</i> control. We optimized a sentinel plot resistance monitoring approach for MON 88702 ThryvOn™ cotton, a new non-high-dose <i>Bt</i> product targeting two sucking pest taxa—<i>Lygus</i> (<i>L. lineolaris</i> and <i>L. hesperus</i>) and thrips (<i>Frankliniella fusca</i> and <i>F. occidentalis</i>)—and report here on the thrips monitoring methods and results. Quantifying thrips immatures was the best metric to characterize the impact of the trait, with at least a 40–60% average reduction of thrips immatures on ThryvOn relative to the control cotton at all sites with higher thrips densities. These data can be used within a ThryvOn resistance monitoring program and represent a case study for establishing a resistance monitoring approach for a non-high-dose trait product.https://www.mdpi.com/2075-4450/14/6/497ThryvOn cottonMON 88702tobacco thripswestern flower thrips<i>Bacillus thuringiensis</i>resistance monitoring |
| spellingShingle | Ashley D. Yates-Stewart Benjamin T. Yorke Alan Willse Jennifer Fridley Graham P. Head Using Sentinel Plots to Monitor for Changes in Thrips Susceptibility to MON 88702 Cotton Containing the Cry51Aa2.834_16 <i>Bt</i> Protein Insects ThryvOn cotton MON 88702 tobacco thrips western flower thrips <i>Bacillus thuringiensis</i> resistance monitoring |
| title | Using Sentinel Plots to Monitor for Changes in Thrips Susceptibility to MON 88702 Cotton Containing the Cry51Aa2.834_16 <i>Bt</i> Protein |
| title_full | Using Sentinel Plots to Monitor for Changes in Thrips Susceptibility to MON 88702 Cotton Containing the Cry51Aa2.834_16 <i>Bt</i> Protein |
| title_fullStr | Using Sentinel Plots to Monitor for Changes in Thrips Susceptibility to MON 88702 Cotton Containing the Cry51Aa2.834_16 <i>Bt</i> Protein |
| title_full_unstemmed | Using Sentinel Plots to Monitor for Changes in Thrips Susceptibility to MON 88702 Cotton Containing the Cry51Aa2.834_16 <i>Bt</i> Protein |
| title_short | Using Sentinel Plots to Monitor for Changes in Thrips Susceptibility to MON 88702 Cotton Containing the Cry51Aa2.834_16 <i>Bt</i> Protein |
| title_sort | using sentinel plots to monitor for changes in thrips susceptibility to mon 88702 cotton containing the cry51aa2 834 16 i bt i protein |
| topic | ThryvOn cotton MON 88702 tobacco thrips western flower thrips <i>Bacillus thuringiensis</i> resistance monitoring |
| url | https://www.mdpi.com/2075-4450/14/6/497 |
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