Recreating the synthesis of starch granules in yeast
Starch, as the major nutritional component of our staple crops and a feedstock for industry, is a vital plant product. It is composed of glucose polymers that form massive semi-crystalline granules. Its precise structure and composition determine its functionality and thus applications; however, the...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2016-11-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/15552 |
| _version_ | 1811227855892250624 |
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| author | Barbara Pfister Antoni Sánchez-Ferrer Ana Diaz Kuanjen Lu Caroline Otto Mirko Holler Farooque Razvi Shaik Florence Meier Raffaele Mezzenga Samuel C Zeeman |
| author_facet | Barbara Pfister Antoni Sánchez-Ferrer Ana Diaz Kuanjen Lu Caroline Otto Mirko Holler Farooque Razvi Shaik Florence Meier Raffaele Mezzenga Samuel C Zeeman |
| author_sort | Barbara Pfister |
| collection | DOAJ |
| description | Starch, as the major nutritional component of our staple crops and a feedstock for industry, is a vital plant product. It is composed of glucose polymers that form massive semi-crystalline granules. Its precise structure and composition determine its functionality and thus applications; however, there is no versatile model system allowing the relationships between the biosynthetic apparatus, glucan structure and properties to be explored. Here, we expressed the core Arabidopsis starch-biosynthesis pathway in Saccharomyces cerevisiae purged of its endogenous glycogen-metabolic enzymes. Systematic variation of the set of biosynthetic enzymes illustrated how each affects glucan structure and solubility. Expression of the complete set resulted in dense, insoluble granules with a starch-like semi-crystalline organization, demonstrating that this system indeed simulates starch biosynthesis. Thus, the yeast system has the potential to accelerate starch research and help create a holistic understanding of starch granule biosynthesis, providing a basis for the targeted biotechnological improvement of crops. |
| first_indexed | 2024-04-12T09:49:03Z |
| format | Article |
| id | doaj.art-9f6a5b8a1a2c4868ae5abaab7a055c57 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T09:49:03Z |
| publishDate | 2016-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-9f6a5b8a1a2c4868ae5abaab7a055c572022-12-22T03:37:53ZengeLife Sciences Publications LtdeLife2050-084X2016-11-01510.7554/eLife.15552Recreating the synthesis of starch granules in yeastBarbara Pfister0Antoni Sánchez-Ferrer1Ana Diaz2Kuanjen Lu3Caroline Otto4Mirko Holler5Farooque Razvi Shaik6Florence Meier7Raffaele Mezzenga8Samuel C Zeeman9https://orcid.org/0000-0002-2791-0915Department of Biology, ETH Zürich, Zürich, SwitzerlandHealth Sciences and Technology, ETH Zürich, Zurich, SwitzerlandPaul Scherrer Institut, Villigen, SwitzerlandDepartment of Biology, ETH Zürich, Zürich, SwitzerlandDepartment of Biology, ETH Zürich, Zürich, SwitzerlandPaul Scherrer Institut, Villigen, SwitzerlandPaul Scherrer Institut, Villigen, SwitzerlandDepartment of Biology, ETH Zürich, Zürich, SwitzerlandHealth Sciences and Technology, ETH Zürich, Zurich, SwitzerlandDepartment of Biology, ETH Zürich, Zürich, SwitzerlandStarch, as the major nutritional component of our staple crops and a feedstock for industry, is a vital plant product. It is composed of glucose polymers that form massive semi-crystalline granules. Its precise structure and composition determine its functionality and thus applications; however, there is no versatile model system allowing the relationships between the biosynthetic apparatus, glucan structure and properties to be explored. Here, we expressed the core Arabidopsis starch-biosynthesis pathway in Saccharomyces cerevisiae purged of its endogenous glycogen-metabolic enzymes. Systematic variation of the set of biosynthetic enzymes illustrated how each affects glucan structure and solubility. Expression of the complete set resulted in dense, insoluble granules with a starch-like semi-crystalline organization, demonstrating that this system indeed simulates starch biosynthesis. Thus, the yeast system has the potential to accelerate starch research and help create a holistic understanding of starch granule biosynthesis, providing a basis for the targeted biotechnological improvement of crops.https://elifesciences.org/articles/15552starch biosynthesismetabolic engineeringamylopectin |
| spellingShingle | Barbara Pfister Antoni Sánchez-Ferrer Ana Diaz Kuanjen Lu Caroline Otto Mirko Holler Farooque Razvi Shaik Florence Meier Raffaele Mezzenga Samuel C Zeeman Recreating the synthesis of starch granules in yeast eLife starch biosynthesis metabolic engineering amylopectin |
| title | Recreating the synthesis of starch granules in yeast |
| title_full | Recreating the synthesis of starch granules in yeast |
| title_fullStr | Recreating the synthesis of starch granules in yeast |
| title_full_unstemmed | Recreating the synthesis of starch granules in yeast |
| title_short | Recreating the synthesis of starch granules in yeast |
| title_sort | recreating the synthesis of starch granules in yeast |
| topic | starch biosynthesis metabolic engineering amylopectin |
| url | https://elifesciences.org/articles/15552 |
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