Carbon dot nanozymes as free radicals scavengers for the management of hepatic ischemia-reperfusion injury by regulating the liver inflammatory network and inhibiting apoptosis
Abstract Background Hepatic ischemia-reperfusion injury (HIRI) is a pathophysiological process during liver transplantation, characterized by insufficient oxygen supply and subsequent restoration of blood flow leading to an overproduction of reactive oxygen species (ROS), which in turn activates the...
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-023-02234-1 |
| _version_ | 1797362934747234304 |
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| author | Haoge Geng Jiayu Chen Kangsheng Tu Hang Tuo Qingsong Wu Jinhui Guo Qingwei Zhu Zhe Zhang Yujie Zhang Dongsheng Huang Mingzhen Zhang Qiuran Xu |
| author_facet | Haoge Geng Jiayu Chen Kangsheng Tu Hang Tuo Qingsong Wu Jinhui Guo Qingwei Zhu Zhe Zhang Yujie Zhang Dongsheng Huang Mingzhen Zhang Qiuran Xu |
| author_sort | Haoge Geng |
| collection | DOAJ |
| description | Abstract Background Hepatic ischemia-reperfusion injury (HIRI) is a pathophysiological process during liver transplantation, characterized by insufficient oxygen supply and subsequent restoration of blood flow leading to an overproduction of reactive oxygen species (ROS), which in turn activates the inflammatory response and leads to cellular damage. Therefore, reducing excess ROS production in the hepatic microenvironment would provide an effective way to mitigate oxidative stress injury and apoptosis during HIRI. Nanozymes with outstanding free radical scavenging activities have aroused great interest and enthusiasm in oxidative stress treatment. Results We previously demonstrated that carbon-dots (C-dots) nanozymes with SOD-like activity could serve as free radicals scavengers. Herein, we proposed that C-dots could protect the liver from ROS-mediated inflammatory responses and apoptosis in HIRI, thereby improving the therapeutic effect. We demonstrated that C-dots with anti-oxidative stress and anti-inflammatory properties improved the survival of L-02 cells under H2O2 and LPS-treated conditions. In the animal model, Our results showed that the impregnation of C-dots could effectively scavenge ROS and reduce the expression of inflammatory cytokines, such as IL-1β, IL-6, IL-12, and TNF-α, resulting in a profound therapeutic effect in the HIRI. To reveal the potential therapeutic mechanism, transcriptome sequencing was performed and the relevant genes were validated, showing that the C-dots exert hepatoprotective effects by modulating the hepatic inflammatory network and inhibiting apoptosis. Conclusions With negligible systemic toxicity, our findings substantiate the potential of C-dots as a therapeutic approach for HIRI, thereby offering a promising intervention strategy for clinical implementation. |
| first_indexed | 2024-03-08T16:14:33Z |
| format | Article |
| id | doaj.art-9f6bbe4ecad54177b082a14a68ffd3e4 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-03-08T16:14:33Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-9f6bbe4ecad54177b082a14a68ffd3e42024-01-07T12:43:01ZengBMCJournal of Nanobiotechnology1477-31552023-12-0121111510.1186/s12951-023-02234-1Carbon dot nanozymes as free radicals scavengers for the management of hepatic ischemia-reperfusion injury by regulating the liver inflammatory network and inhibiting apoptosisHaoge Geng0Jiayu Chen1Kangsheng Tu2Hang Tuo3Qingsong Wu4Jinhui Guo5Qingwei Zhu6Zhe Zhang7Yujie Zhang8Dongsheng Huang9Mingzhen Zhang10Qiuran Xu11Laboratory of Tumor Molecular Diagnosis and Individualized Medicine of Zhejiang Province, Affiliated People’s Hospital, Zhejiang Provincial People’s Hospital, Hangzhou Medical CollegeDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Xi’an Jiaotong UniversityThe Second Clinical Medical College, Zhejiang Chinese Medical UniversityQingdao Medical College, Qingdao UniversityQingdao Medical College, Qingdao UniversityThe Second Clinical Medical College, Zhejiang Chinese Medical UniversitySchool of Basic Medical Sciences, Xi’an Jiaotong UniversityLaboratory of Tumor Molecular Diagnosis and Individualized Medicine of Zhejiang Province, Affiliated People’s Hospital, Zhejiang Provincial People’s Hospital, Hangzhou Medical CollegeSchool of Basic Medical Sciences, Xi’an Jiaotong UniversityLaboratory of Tumor Molecular Diagnosis and Individualized Medicine of Zhejiang Province, Affiliated People’s Hospital, Zhejiang Provincial People’s Hospital, Hangzhou Medical CollegeAbstract Background Hepatic ischemia-reperfusion injury (HIRI) is a pathophysiological process during liver transplantation, characterized by insufficient oxygen supply and subsequent restoration of blood flow leading to an overproduction of reactive oxygen species (ROS), which in turn activates the inflammatory response and leads to cellular damage. Therefore, reducing excess ROS production in the hepatic microenvironment would provide an effective way to mitigate oxidative stress injury and apoptosis during HIRI. Nanozymes with outstanding free radical scavenging activities have aroused great interest and enthusiasm in oxidative stress treatment. Results We previously demonstrated that carbon-dots (C-dots) nanozymes with SOD-like activity could serve as free radicals scavengers. Herein, we proposed that C-dots could protect the liver from ROS-mediated inflammatory responses and apoptosis in HIRI, thereby improving the therapeutic effect. We demonstrated that C-dots with anti-oxidative stress and anti-inflammatory properties improved the survival of L-02 cells under H2O2 and LPS-treated conditions. In the animal model, Our results showed that the impregnation of C-dots could effectively scavenge ROS and reduce the expression of inflammatory cytokines, such as IL-1β, IL-6, IL-12, and TNF-α, resulting in a profound therapeutic effect in the HIRI. To reveal the potential therapeutic mechanism, transcriptome sequencing was performed and the relevant genes were validated, showing that the C-dots exert hepatoprotective effects by modulating the hepatic inflammatory network and inhibiting apoptosis. Conclusions With negligible systemic toxicity, our findings substantiate the potential of C-dots as a therapeutic approach for HIRI, thereby offering a promising intervention strategy for clinical implementation.https://doi.org/10.1186/s12951-023-02234-1C-dots nanozymesReactive oxygen species (ROS)Hepatic ischemia-reperfusion injury (HIRI)Inflammatory networkOxidative stress |
| spellingShingle | Haoge Geng Jiayu Chen Kangsheng Tu Hang Tuo Qingsong Wu Jinhui Guo Qingwei Zhu Zhe Zhang Yujie Zhang Dongsheng Huang Mingzhen Zhang Qiuran Xu Carbon dot nanozymes as free radicals scavengers for the management of hepatic ischemia-reperfusion injury by regulating the liver inflammatory network and inhibiting apoptosis Journal of Nanobiotechnology C-dots nanozymes Reactive oxygen species (ROS) Hepatic ischemia-reperfusion injury (HIRI) Inflammatory network Oxidative stress |
| title | Carbon dot nanozymes as free radicals scavengers for the management of hepatic ischemia-reperfusion injury by regulating the liver inflammatory network and inhibiting apoptosis |
| title_full | Carbon dot nanozymes as free radicals scavengers for the management of hepatic ischemia-reperfusion injury by regulating the liver inflammatory network and inhibiting apoptosis |
| title_fullStr | Carbon dot nanozymes as free radicals scavengers for the management of hepatic ischemia-reperfusion injury by regulating the liver inflammatory network and inhibiting apoptosis |
| title_full_unstemmed | Carbon dot nanozymes as free radicals scavengers for the management of hepatic ischemia-reperfusion injury by regulating the liver inflammatory network and inhibiting apoptosis |
| title_short | Carbon dot nanozymes as free radicals scavengers for the management of hepatic ischemia-reperfusion injury by regulating the liver inflammatory network and inhibiting apoptosis |
| title_sort | carbon dot nanozymes as free radicals scavengers for the management of hepatic ischemia reperfusion injury by regulating the liver inflammatory network and inhibiting apoptosis |
| topic | C-dots nanozymes Reactive oxygen species (ROS) Hepatic ischemia-reperfusion injury (HIRI) Inflammatory network Oxidative stress |
| url | https://doi.org/10.1186/s12951-023-02234-1 |
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