Hairy root culture of Lactuca virosa L.
A hairy root culture of Lactuca virosa L. (Asteraceae) was initiated by infection of the leaf explants of the aseptic plants with Agrobacterium rhizogenes - strain LBA 9402. One of the obtained clones which showed the most favourable growth rate was used for further investigation. An influence of di...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2014-01-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/977 |
| _version_ | 1811223532532662272 |
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| author | Anna Stojakowska Janusz Malarz Wanda Kisiel Stanisław Kohlmünzer |
| author_facet | Anna Stojakowska Janusz Malarz Wanda Kisiel Stanisław Kohlmünzer |
| author_sort | Anna Stojakowska |
| collection | DOAJ |
| description | A hairy root culture of Lactuca virosa L. (Asteraceae) was initiated by infection of the leaf explants of the aseptic plants with Agrobacterium rhizogenes - strain LBA 9402. One of the obtained clones which showed the most favourable growth rate was used for further investigation. An influence of different nutrient media and sucrose concentrations on the biomass accumulation was studied. The most advantageous for the biomass increment were media: MS and MS containing macronutrients reduced to half concentration, supplemented with 5% sucrose. A preliminary chromatographic analysis of the tissue extract revealed the presence of sesquiterpene lactones characteristic of the roots of the intact plant. |
| first_indexed | 2024-04-12T08:34:17Z |
| format | Article |
| id | doaj.art-9f6c94f0b52f46a49e6b010f7c1584c1 |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-04-12T08:34:17Z |
| publishDate | 2014-01-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-9f6c94f0b52f46a49e6b010f7c1584c12022-12-22T03:40:04ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802014-01-01641333910.5586/asbp.1995.006737Hairy root culture of Lactuca virosa L.Anna Stojakowska0Janusz Malarz1Wanda Kisiel2Stanisław Kohlmünzer3Polish Academy of SciencesPolish Academy of SciencesPolish Academy of SciencesPolish Academy of SciencesA hairy root culture of Lactuca virosa L. (Asteraceae) was initiated by infection of the leaf explants of the aseptic plants with Agrobacterium rhizogenes - strain LBA 9402. One of the obtained clones which showed the most favourable growth rate was used for further investigation. An influence of different nutrient media and sucrose concentrations on the biomass accumulation was studied. The most advantageous for the biomass increment were media: MS and MS containing macronutrients reduced to half concentration, supplemented with 5% sucrose. A preliminary chromatographic analysis of the tissue extract revealed the presence of sesquiterpene lactones characteristic of the roots of the intact plant.https://pbsociety.org.pl/journals/index.php/asbp/article/view/977Lactuca virosa L.Agrobacterium rhizogeneshairy rootssesquiterpene lactones |
| spellingShingle | Anna Stojakowska Janusz Malarz Wanda Kisiel Stanisław Kohlmünzer Hairy root culture of Lactuca virosa L. Acta Societatis Botanicorum Poloniae Lactuca virosa L. Agrobacterium rhizogenes hairy roots sesquiterpene lactones |
| title | Hairy root culture of Lactuca virosa L. |
| title_full | Hairy root culture of Lactuca virosa L. |
| title_fullStr | Hairy root culture of Lactuca virosa L. |
| title_full_unstemmed | Hairy root culture of Lactuca virosa L. |
| title_short | Hairy root culture of Lactuca virosa L. |
| title_sort | hairy root culture of lactuca virosa l |
| topic | Lactuca virosa L. Agrobacterium rhizogenes hairy roots sesquiterpene lactones |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/977 |
| work_keys_str_mv | AT annastojakowska hairyrootcultureoflactucavirosal AT januszmalarz hairyrootcultureoflactucavirosal AT wandakisiel hairyrootcultureoflactucavirosal AT stanisławkohlmunzer hairyrootcultureoflactucavirosal |