Characterization of a Robust and pH-Stable Tannase from Mangrove-Derived Yeast <i>Rhodosporidium diobovatum</i> Q95
Tannase plays a crucial role in many fields, such as the pharmaceutical industry, beverage processing, and brewing. Although many tannases derived from bacteria and fungi have been thoroughly studied, those with good pH stabilities are still less reported. In this work, a mangrove-derived yeast stra...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/11/546 |
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| author | Jie Pan Ni-Na Wang Xue-Jing Yin Xiao-Ling Liang Zhi-Peng Wang |
| author_facet | Jie Pan Ni-Na Wang Xue-Jing Yin Xiao-Ling Liang Zhi-Peng Wang |
| author_sort | Jie Pan |
| collection | DOAJ |
| description | Tannase plays a crucial role in many fields, such as the pharmaceutical industry, beverage processing, and brewing. Although many tannases derived from bacteria and fungi have been thoroughly studied, those with good pH stabilities are still less reported. In this work, a mangrove-derived yeast strain <i>Rhodosporidium diobovatum</i> Q95, capable of efficiently degrading tannin, was screened to induce tannase, which exhibited an activity of up to 26.4 U/mL after 48 h cultivation in the presence of 15 g/L tannic acid. The tannase coding gene <i>TANRD</i> was cloned and expressed in <i>Yarrowia lipolytica</i>. The activity of recombinant tannase (named TanRd) was as high as 27.3 U/mL. TanRd was purified by chromatography and analysed by SDS-PAGE, showing a molecular weight of 75.1 kDa. The specific activity of TanRd towards tannic acid was 676.4 U/mg. Its highest activity was obtained at 40 °C, with more than 70% of the activity observed at 25–60 °C. Furthermore, it possessed at least 60% of the activity in a broad pH range of 2.5–6.5. Notably, TanRd was excellently stable at a pH range from 3.0 to 8.0; over 65% of its maximum activity remained after incubation. Besides, the broad substrate specificity of TanRd to esters of gallic acid has attracted wide attention. In view of the above, tannase resources were developed from mangrove-derived yeasts for the first time in this study. This tannase can become a promising material in tannin biodegradation and gallic acid production. |
| first_indexed | 2024-03-10T15:12:11Z |
| format | Article |
| id | doaj.art-9f6f569440f844ecb46f9d43b95852ab |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T15:12:11Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-9f6f569440f844ecb46f9d43b95852ab2023-11-20T19:17:57ZengMDPI AGMarine Drugs1660-33972020-10-01181154610.3390/md18110546Characterization of a Robust and pH-Stable Tannase from Mangrove-Derived Yeast <i>Rhodosporidium diobovatum</i> Q95Jie Pan0Ni-Na Wang1Xue-Jing Yin2Xiao-Ling Liang3Zhi-Peng Wang4Qingdao Mental Health Center, Qingdao University, Qingdao 266034, ChinaQingdao Mental Health Center, Qingdao University, Qingdao 266034, ChinaQingdao Mental Health Center, Qingdao University, Qingdao 266034, ChinaQingdao Mental Health Center, Qingdao University, Qingdao 266034, ChinaMarine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, ChinaTannase plays a crucial role in many fields, such as the pharmaceutical industry, beverage processing, and brewing. Although many tannases derived from bacteria and fungi have been thoroughly studied, those with good pH stabilities are still less reported. In this work, a mangrove-derived yeast strain <i>Rhodosporidium diobovatum</i> Q95, capable of efficiently degrading tannin, was screened to induce tannase, which exhibited an activity of up to 26.4 U/mL after 48 h cultivation in the presence of 15 g/L tannic acid. The tannase coding gene <i>TANRD</i> was cloned and expressed in <i>Yarrowia lipolytica</i>. The activity of recombinant tannase (named TanRd) was as high as 27.3 U/mL. TanRd was purified by chromatography and analysed by SDS-PAGE, showing a molecular weight of 75.1 kDa. The specific activity of TanRd towards tannic acid was 676.4 U/mg. Its highest activity was obtained at 40 °C, with more than 70% of the activity observed at 25–60 °C. Furthermore, it possessed at least 60% of the activity in a broad pH range of 2.5–6.5. Notably, TanRd was excellently stable at a pH range from 3.0 to 8.0; over 65% of its maximum activity remained after incubation. Besides, the broad substrate specificity of TanRd to esters of gallic acid has attracted wide attention. In view of the above, tannase resources were developed from mangrove-derived yeasts for the first time in this study. This tannase can become a promising material in tannin biodegradation and gallic acid production.https://www.mdpi.com/1660-3397/18/11/546tannasepH stabilitytannin biodegradationgallic acid |
| spellingShingle | Jie Pan Ni-Na Wang Xue-Jing Yin Xiao-Ling Liang Zhi-Peng Wang Characterization of a Robust and pH-Stable Tannase from Mangrove-Derived Yeast <i>Rhodosporidium diobovatum</i> Q95 Marine Drugs tannase pH stability tannin biodegradation gallic acid |
| title | Characterization of a Robust and pH-Stable Tannase from Mangrove-Derived Yeast <i>Rhodosporidium diobovatum</i> Q95 |
| title_full | Characterization of a Robust and pH-Stable Tannase from Mangrove-Derived Yeast <i>Rhodosporidium diobovatum</i> Q95 |
| title_fullStr | Characterization of a Robust and pH-Stable Tannase from Mangrove-Derived Yeast <i>Rhodosporidium diobovatum</i> Q95 |
| title_full_unstemmed | Characterization of a Robust and pH-Stable Tannase from Mangrove-Derived Yeast <i>Rhodosporidium diobovatum</i> Q95 |
| title_short | Characterization of a Robust and pH-Stable Tannase from Mangrove-Derived Yeast <i>Rhodosporidium diobovatum</i> Q95 |
| title_sort | characterization of a robust and ph stable tannase from mangrove derived yeast i rhodosporidium diobovatum i q95 |
| topic | tannase pH stability tannin biodegradation gallic acid |
| url | https://www.mdpi.com/1660-3397/18/11/546 |
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