Protocol for bulk RNA sequencing of enriched human neutrophils from whole blood and estimation of sample purity

Protocol for bulk RNA sequencing of enriched human neutrophils from whole blood and estimation of sample purity

Summary: Although neutrophils are the most abundant leukocyte in healthy individuals and impact outcomes of diseases ranging from sepsis to cancer, they remain understudied due to technical constraints of isolation, preservation, and sequencing. We present a modified Smart-Seq2 protocol for bulk RNA...

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Bibliographic Details
Main Authors: Anna L.K. Gonye, Thomas J. LaSalle, Samuel S. Freeman, Miguel Reyes, Nir Hacohen, Alexandra-Chloé Villani, Moshe Sade-Feldman
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Bioinformatics
Sequence Analysis
Cell Biology
Cell Isolation
Single Cell
RNAseq
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723000837

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http://www.sciencedirect.com/science/article/pii/S2666166723000837

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