Microbiological Evaluation and Sperm DNA Fragmentation in Semen Samples of Patients Undergoing Fertility Investigation
Fifteen percent of male infertility is associated with urogenital infections; several pathogens are able to alter the testicular and accessory glands’ microenvironment, resulting in the impairment of biofunctional sperm parameters. The purpose of this study was to assess the influence of urogenital...
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MDPI AG
2021-04-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/5/654 |
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| author | Chiara Pagliuca Federica Cariati Francesca Bagnulo Elena Scaglione Consolata Carotenuto Fabrizio Farina Valeria D’Argenio Francesca Carraturo Paola D’Aprile Mariateresa Vitiello Ida Strina Carlo Alviggi Roberta Colicchio Rossella Tomaiuolo Paola Salvatore |
| author_facet | Chiara Pagliuca Federica Cariati Francesca Bagnulo Elena Scaglione Consolata Carotenuto Fabrizio Farina Valeria D’Argenio Francesca Carraturo Paola D’Aprile Mariateresa Vitiello Ida Strina Carlo Alviggi Roberta Colicchio Rossella Tomaiuolo Paola Salvatore |
| author_sort | Chiara Pagliuca |
| collection | DOAJ |
| description | Fifteen percent of male infertility is associated with urogenital infections; several pathogens are able to alter the testicular and accessory glands’ microenvironment, resulting in the impairment of biofunctional sperm parameters. The purpose of this study was to assess the influence of urogenital infections on the quality of 53 human semen samples through standard analysis, microbiological evaluation, and molecular characterization of sperm DNA damage. The results showed a significant correlation between infected status and semen volume, sperm concentration, and motility. Moreover, a high risk of fragmented sperm DNA was demonstrated in the altered semen samples. Urogenital infections are often asymptomatic and thus an in-depth evaluation of the seminal sample can allow for both the diagnosis and therapy of infections while providing more indicators for male infertility management. |
| first_indexed | 2024-03-10T11:54:19Z |
| format | Article |
| id | doaj.art-9f74d8f1a45e41158f5fcf8bbe71fbb0 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T11:54:19Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-9f74d8f1a45e41158f5fcf8bbe71fbb02023-11-21T17:25:33ZengMDPI AGGenes2073-44252021-04-0112565410.3390/genes12050654Microbiological Evaluation and Sperm DNA Fragmentation in Semen Samples of Patients Undergoing Fertility InvestigationChiara Pagliuca0Federica Cariati1Francesca Bagnulo2Elena Scaglione3Consolata Carotenuto4Fabrizio Farina5Valeria D’Argenio6Francesca Carraturo7Paola D’Aprile8Mariateresa Vitiello9Ida Strina10Carlo Alviggi11Roberta Colicchio12Rossella Tomaiuolo13Paola Salvatore14Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, via S. Pansini 5, 80131 Napoli, ItalyCEINGE-Biotecnologie Avanzate, via G. Salvatore 486, 80145 Napoli, ItalyFertility Unit, Maternal-Child Department, AOU Policlinico Federico II, 80131 Naples, ItalyDepartment of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, via S. Pansini 5, 80131 Napoli, ItalyDepartment of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, via S. Pansini 5, 80131 Napoli, ItalyDepartment of Law, Economics, Management and Quantitative Methods, University of Sannio, 82100 Benevento, ItalyCEINGE-Biotecnologie Avanzate, via G. Salvatore 486, 80145 Napoli, ItalyDepartment of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, via S. Pansini 5, 80131 Napoli, ItalyDepartment of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, via S. Pansini 5, 80131 Napoli, ItalyDepartment of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, via S. Pansini 5, 80131 Napoli, ItalyFertility Unit, Maternal-Child Department, AOU Policlinico Federico II, 80131 Naples, ItalyFertility Unit, Maternal-Child Department, AOU Policlinico Federico II, 80131 Naples, ItalyDepartment of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, via S. Pansini 5, 80131 Napoli, ItalyUniversità Vita-Salute San Raffaele, 20132 Milan, ItalyDepartment of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, via S. Pansini 5, 80131 Napoli, ItalyFifteen percent of male infertility is associated with urogenital infections; several pathogens are able to alter the testicular and accessory glands’ microenvironment, resulting in the impairment of biofunctional sperm parameters. The purpose of this study was to assess the influence of urogenital infections on the quality of 53 human semen samples through standard analysis, microbiological evaluation, and molecular characterization of sperm DNA damage. The results showed a significant correlation between infected status and semen volume, sperm concentration, and motility. Moreover, a high risk of fragmented sperm DNA was demonstrated in the altered semen samples. Urogenital infections are often asymptomatic and thus an in-depth evaluation of the seminal sample can allow for both the diagnosis and therapy of infections while providing more indicators for male infertility management.https://www.mdpi.com/2073-4425/12/5/654urogenital infectionsmicrobiological evaluationsperm DNA fragmentationmale infertility |
| spellingShingle | Chiara Pagliuca Federica Cariati Francesca Bagnulo Elena Scaglione Consolata Carotenuto Fabrizio Farina Valeria D’Argenio Francesca Carraturo Paola D’Aprile Mariateresa Vitiello Ida Strina Carlo Alviggi Roberta Colicchio Rossella Tomaiuolo Paola Salvatore Microbiological Evaluation and Sperm DNA Fragmentation in Semen Samples of Patients Undergoing Fertility Investigation Genes urogenital infections microbiological evaluation sperm DNA fragmentation male infertility |
| title | Microbiological Evaluation and Sperm DNA Fragmentation in Semen Samples of Patients Undergoing Fertility Investigation |
| title_full | Microbiological Evaluation and Sperm DNA Fragmentation in Semen Samples of Patients Undergoing Fertility Investigation |
| title_fullStr | Microbiological Evaluation and Sperm DNA Fragmentation in Semen Samples of Patients Undergoing Fertility Investigation |
| title_full_unstemmed | Microbiological Evaluation and Sperm DNA Fragmentation in Semen Samples of Patients Undergoing Fertility Investigation |
| title_short | Microbiological Evaluation and Sperm DNA Fragmentation in Semen Samples of Patients Undergoing Fertility Investigation |
| title_sort | microbiological evaluation and sperm dna fragmentation in semen samples of patients undergoing fertility investigation |
| topic | urogenital infections microbiological evaluation sperm DNA fragmentation male infertility |
| url | https://www.mdpi.com/2073-4425/12/5/654 |
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