Identification of COVID-19 B-cell epitopes with phage-displayed peptide library
Abstract Background Coronavirus disease 19 (COVID-19) first appeared in the city of Wuhan, in the Hubei province of China. Since its emergence, the COVID-19-causing virus, SARS-CoV-2, has been rapidly transmitted around the globe, overwhelming the medical care systems in many countries and leading t...
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | Journal of Biomedical Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12929-021-00740-8 |
| _version_ | 1818440903621083136 |
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| author | Jing-You Guo I-Ju Liu Hsiu-Ting Lin Mei-Jung Wang Yu-Ling Chang Shin-Chang Lin Mei-Ying Liao Wei-Chia Hsu Yi-Ling Lin James C. Liao Han-Chung Wu |
| author_facet | Jing-You Guo I-Ju Liu Hsiu-Ting Lin Mei-Jung Wang Yu-Ling Chang Shin-Chang Lin Mei-Ying Liao Wei-Chia Hsu Yi-Ling Lin James C. Liao Han-Chung Wu |
| author_sort | Jing-You Guo |
| collection | DOAJ |
| description | Abstract Background Coronavirus disease 19 (COVID-19) first appeared in the city of Wuhan, in the Hubei province of China. Since its emergence, the COVID-19-causing virus, SARS-CoV-2, has been rapidly transmitted around the globe, overwhelming the medical care systems in many countries and leading to more than 3.3 million deaths. Identification of immunological epitopes on the virus would be highly useful for the development of diagnostic tools and vaccines that will be critical to limiting further spread of COVID-19. Methods To find disease-specific B-cell epitopes that correspond to or mimic natural epitopes, we used phage display technology to determine the targets of specific antibodies present in the sera of immune-responsive COVID-19 patients. Enzyme-linked immunosorbent assays were further applied to assess competitive antibody binding and serological detection. VaxiJen, BepiPred-2.0 and DiscoTope 2.0 were utilized for B-cell epitope prediction. PyMOL was used for protein structural analysis. Results 36 enriched peptides were identified by biopanning with antibodies from two COVID-19 patients; the peptides 4 motifs with consensus residues corresponding to two potential B-cell epitopes on SARS-CoV-2 viral proteins. The putative epitopes and hit peptides were then synthesized for validation by competitive antibody binding and serological detection. Conclusions The identified B-cell epitopes on SARS-CoV-2 may aid investigations into COVID-19 pathogenesis and facilitate the development of epitope-based serological diagnostics and vaccines. |
| first_indexed | 2024-12-14T18:19:45Z |
| format | Article |
| id | doaj.art-9f76db302e484e87859cd59a8355dd12 |
| institution | Directory Open Access Journal |
| issn | 1423-0127 |
| language | English |
| last_indexed | 2024-12-14T18:19:45Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biomedical Science |
| spelling | doaj.art-9f76db302e484e87859cd59a8355dd122022-12-21T22:52:06ZengBMCJournal of Biomedical Science1423-01272021-06-0128111310.1186/s12929-021-00740-8Identification of COVID-19 B-cell epitopes with phage-displayed peptide libraryJing-You Guo0I-Ju Liu1Hsiu-Ting Lin2Mei-Jung Wang3Yu-Ling Chang4Shin-Chang Lin5Mei-Ying Liao6Wei-Chia Hsu7Yi-Ling Lin8James C. Liao9Han-Chung Wu10Institute of Biologic Chemistry, Academia SinicaInstitute of Cellular and Organismic Biology, Academia SinicaInstitute of Cellular and Organismic Biology, Academia SinicaInstitute of Cellular and Organismic Biology, Academia SinicaInstitute of Cellular and Organismic Biology, Academia SinicaInstitute of Cellular and Organismic Biology, Academia SinicaInstitute of Cellular and Organismic Biology, Academia SinicaInstitute of Cellular and Organismic Biology, Academia SinicaInstitute of Biomedical Sciences, Academia SinicaInstitute of Biologic Chemistry, Academia SinicaInstitute of Cellular and Organismic Biology, Academia SinicaAbstract Background Coronavirus disease 19 (COVID-19) first appeared in the city of Wuhan, in the Hubei province of China. Since its emergence, the COVID-19-causing virus, SARS-CoV-2, has been rapidly transmitted around the globe, overwhelming the medical care systems in many countries and leading to more than 3.3 million deaths. Identification of immunological epitopes on the virus would be highly useful for the development of diagnostic tools and vaccines that will be critical to limiting further spread of COVID-19. Methods To find disease-specific B-cell epitopes that correspond to or mimic natural epitopes, we used phage display technology to determine the targets of specific antibodies present in the sera of immune-responsive COVID-19 patients. Enzyme-linked immunosorbent assays were further applied to assess competitive antibody binding and serological detection. VaxiJen, BepiPred-2.0 and DiscoTope 2.0 were utilized for B-cell epitope prediction. PyMOL was used for protein structural analysis. Results 36 enriched peptides were identified by biopanning with antibodies from two COVID-19 patients; the peptides 4 motifs with consensus residues corresponding to two potential B-cell epitopes on SARS-CoV-2 viral proteins. The putative epitopes and hit peptides were then synthesized for validation by competitive antibody binding and serological detection. Conclusions The identified B-cell epitopes on SARS-CoV-2 may aid investigations into COVID-19 pathogenesis and facilitate the development of epitope-based serological diagnostics and vaccines.https://doi.org/10.1186/s12929-021-00740-8COVID-19SARS-CoV-2Phage-displayed peptidesB-cell epitopeDiagnostic toolSerological detection |
| spellingShingle | Jing-You Guo I-Ju Liu Hsiu-Ting Lin Mei-Jung Wang Yu-Ling Chang Shin-Chang Lin Mei-Ying Liao Wei-Chia Hsu Yi-Ling Lin James C. Liao Han-Chung Wu Identification of COVID-19 B-cell epitopes with phage-displayed peptide library Journal of Biomedical Science COVID-19 SARS-CoV-2 Phage-displayed peptides B-cell epitope Diagnostic tool Serological detection |
| title | Identification of COVID-19 B-cell epitopes with phage-displayed peptide library |
| title_full | Identification of COVID-19 B-cell epitopes with phage-displayed peptide library |
| title_fullStr | Identification of COVID-19 B-cell epitopes with phage-displayed peptide library |
| title_full_unstemmed | Identification of COVID-19 B-cell epitopes with phage-displayed peptide library |
| title_short | Identification of COVID-19 B-cell epitopes with phage-displayed peptide library |
| title_sort | identification of covid 19 b cell epitopes with phage displayed peptide library |
| topic | COVID-19 SARS-CoV-2 Phage-displayed peptides B-cell epitope Diagnostic tool Serological detection |
| url | https://doi.org/10.1186/s12929-021-00740-8 |
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