Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue Engineering
For bone tissue engineering, stem cell-based therapy has become a promising option. Recently, cell transplantation supported by polymeric carriers has been increasingly evaluated. Herein, we encapsulated human olfactory ectomesenchymal stem cells (OE-MSC) in the collagen hydrogel system, and their o...
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2021-07-01
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| author | Sara Simorgh Peiman Brouki Milan Maryam Saadatmand Zohreh Bagher Mazaher Gholipourmalekabadi Rafieh Alizadeh Ahmad Hivechi Zohreh Arabpour Masoud Hamidi Cédric Delattre |
| author_facet | Sara Simorgh Peiman Brouki Milan Maryam Saadatmand Zohreh Bagher Mazaher Gholipourmalekabadi Rafieh Alizadeh Ahmad Hivechi Zohreh Arabpour Masoud Hamidi Cédric Delattre |
| author_sort | Sara Simorgh |
| collection | DOAJ |
| description | For bone tissue engineering, stem cell-based therapy has become a promising option. Recently, cell transplantation supported by polymeric carriers has been increasingly evaluated. Herein, we encapsulated human olfactory ectomesenchymal stem cells (OE-MSC) in the collagen hydrogel system, and their osteogenic potential was assessed in vitro and in vivo conditions. Collagen type I was composed of four different concentrations of (4 mg/mL, 5 mg/mL, 6 mg/mL, 7 mg/mL). SDS-Page, FTIR, rheologic test, resazurin assay, live/dead assay, and SEM were used to characterize collagen hydrogels. OE-MSCs encapsulated in the optimum concentration of collagen hydrogel and transplanted in rat calvarial defects. The tissue samples were harvested after 4- and 8-weeks post-transplantation and assessed by optical imaging, micro CT, and H&E staining methods. The highest porosity and biocompatibility were confirmed in all scaffolds. The collagen hydrogel with 7 mg/mL concentration was presented as optimal mechanical properties close to the naïve bone. Furthermore, the same concentration illustrated high osteogenic differentiation confirmed by real-time PCR and alizarin red S methods. Bone healing has significantly occurred in defects treated with OE-MSCs encapsulated hydrogels in vivo. As a result, OE-MSCs with suitable carriers could be used as an appropriate cell source to address clinical bone complications. |
| first_indexed | 2024-03-10T09:33:37Z |
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| id | doaj.art-9f79e90b9eb44a9e9397232b1312ff7a |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T09:33:37Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-9f79e90b9eb44a9e9397232b1312ff7a2023-11-22T04:16:39ZengMDPI AGMaterials1996-19442021-07-011414390910.3390/ma14143909Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue EngineeringSara Simorgh0Peiman Brouki Milan1Maryam Saadatmand2Zohreh Bagher3Mazaher Gholipourmalekabadi4Rafieh Alizadeh5Ahmad Hivechi6Zohreh Arabpour7Masoud Hamidi8Cédric Delattre9Cellular and Molecular Research Centre, Iran University of Medical Sciences, Tehran 1591639675, IranCellular and Molecular Research Centre, Iran University of Medical Sciences, Tehran 1591639675, IranDepartment of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran 111559465, IranDepartment of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran 1591639675, IranCellular and Molecular Research Centre, Iran University of Medical Sciences, Tehran 1591639675, IranENT and Head and Neck Research Center and Department, Hazrat Rasoul Akram Hospital, The Five Senses Health Institute, Iran University of Medical Sciences, Tehran 1445613111, IranCellular and Molecular Research Centre, Iran University of Medical Sciences, Tehran 1591639675, IranDepartment of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran 1417613151, IranDepartment of Medical Biotechnology, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht 4477166595, IranUniversité Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, F-63000 Clermont-Ferrand, FranceFor bone tissue engineering, stem cell-based therapy has become a promising option. Recently, cell transplantation supported by polymeric carriers has been increasingly evaluated. Herein, we encapsulated human olfactory ectomesenchymal stem cells (OE-MSC) in the collagen hydrogel system, and their osteogenic potential was assessed in vitro and in vivo conditions. Collagen type I was composed of four different concentrations of (4 mg/mL, 5 mg/mL, 6 mg/mL, 7 mg/mL). SDS-Page, FTIR, rheologic test, resazurin assay, live/dead assay, and SEM were used to characterize collagen hydrogels. OE-MSCs encapsulated in the optimum concentration of collagen hydrogel and transplanted in rat calvarial defects. The tissue samples were harvested after 4- and 8-weeks post-transplantation and assessed by optical imaging, micro CT, and H&E staining methods. The highest porosity and biocompatibility were confirmed in all scaffolds. The collagen hydrogel with 7 mg/mL concentration was presented as optimal mechanical properties close to the naïve bone. Furthermore, the same concentration illustrated high osteogenic differentiation confirmed by real-time PCR and alizarin red S methods. Bone healing has significantly occurred in defects treated with OE-MSCs encapsulated hydrogels in vivo. As a result, OE-MSCs with suitable carriers could be used as an appropriate cell source to address clinical bone complications.https://www.mdpi.com/1996-1944/14/14/3909tissue engineeringcollagen hydrogelcell deliverybone regenerationolfactory ectomesenchyme stem cells |
| spellingShingle | Sara Simorgh Peiman Brouki Milan Maryam Saadatmand Zohreh Bagher Mazaher Gholipourmalekabadi Rafieh Alizadeh Ahmad Hivechi Zohreh Arabpour Masoud Hamidi Cédric Delattre Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue Engineering Materials tissue engineering collagen hydrogel cell delivery bone regeneration olfactory ectomesenchyme stem cells |
| title | Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue Engineering |
| title_full | Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue Engineering |
| title_fullStr | Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue Engineering |
| title_full_unstemmed | Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue Engineering |
| title_short | Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue Engineering |
| title_sort | human olfactory mucosa stem cells delivery using a collagen hydrogel as a potential candidate for bone tissue engineering |
| topic | tissue engineering collagen hydrogel cell delivery bone regeneration olfactory ectomesenchyme stem cells |
| url | https://www.mdpi.com/1996-1944/14/14/3909 |
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