The Tannins from <i>Sanguisorba officinalis</i> L. (Rosaceae): A Systematic Study on the Metabolites of Rats Based on HPLC–LTQ–Orbitrap MS<sup>2</sup> Analysis
<i>Sanguisorba</i> tannins are the major active ingredients in <i>Sanguisorba ofJicinalis</i> L. (Rosaceae), one of the most popular herbal medicines in China, is widely prescribed for hemostasis. In this study, three kinds of tannins extract from <i>Sanguisorba officin...
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MDPI AG
2021-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/13/4053 |
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| author | Jingjing Tu Qiaoling Li Benhong Zhou |
| author_facet | Jingjing Tu Qiaoling Li Benhong Zhou |
| author_sort | Jingjing Tu |
| collection | DOAJ |
| description | <i>Sanguisorba</i> tannins are the major active ingredients in <i>Sanguisorba ofJicinalis</i> L. (Rosaceae), one of the most popular herbal medicines in China, is widely prescribed for hemostasis. In this study, three kinds of tannins extract from <i>Sanguisorba officinalis</i> L. (Rosaceae), and the metabolites in vivo and in vitro were detected and identified by high-pressure liquid chromatography, coupled with linear ion trap orbitrap tandem mass spectrometry (HPLC–LTQ–Orbitrap). For in vivo assessment, the rats were administered at a single dose of 150 mg/kg, after which 12 metabolites were found in urine, 6 metabolites were found in feces, and 8 metabolites were found in bile, while metabolites were barely found in plasma and tissues. For in vitro assessment, 100 μM <i>Sanguisorba</i> tannins were incubated with rat liver microsomes, liver cytosol, and feces, after which nine metabolites were found in intestinal microbiota and five metabolites were found in liver microsomes and liver cytosol. Moreover, the metabolic pathways of <i>Sanguisorba</i> tannins were proposed, which shed light on their mechanism. |
| first_indexed | 2024-03-10T09:51:01Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T09:51:01Z |
| publishDate | 2021-07-01 |
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| series | Molecules |
| spelling | doaj.art-9f7b8a2a982740829902676f0c344e9c2023-11-22T02:47:20ZengMDPI AGMolecules1420-30492021-07-012613405310.3390/molecules26134053The Tannins from <i>Sanguisorba officinalis</i> L. (Rosaceae): A Systematic Study on the Metabolites of Rats Based on HPLC–LTQ–Orbitrap MS<sup>2</sup> AnalysisJingjing Tu0Qiaoling Li1Benhong Zhou2Department of Pharmacy, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Pharmacy, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Pharmacy, Renmin Hospital of Wuhan University, Wuhan 430060, China<i>Sanguisorba</i> tannins are the major active ingredients in <i>Sanguisorba ofJicinalis</i> L. (Rosaceae), one of the most popular herbal medicines in China, is widely prescribed for hemostasis. In this study, three kinds of tannins extract from <i>Sanguisorba officinalis</i> L. (Rosaceae), and the metabolites in vivo and in vitro were detected and identified by high-pressure liquid chromatography, coupled with linear ion trap orbitrap tandem mass spectrometry (HPLC–LTQ–Orbitrap). For in vivo assessment, the rats were administered at a single dose of 150 mg/kg, after which 12 metabolites were found in urine, 6 metabolites were found in feces, and 8 metabolites were found in bile, while metabolites were barely found in plasma and tissues. For in vitro assessment, 100 μM <i>Sanguisorba</i> tannins were incubated with rat liver microsomes, liver cytosol, and feces, after which nine metabolites were found in intestinal microbiota and five metabolites were found in liver microsomes and liver cytosol. Moreover, the metabolic pathways of <i>Sanguisorba</i> tannins were proposed, which shed light on their mechanism.https://www.mdpi.com/1420-3049/26/13/4053<i>Sanguisorba</i> tanninsHPLC–LTQ–Orbitrap MS<sup>2</sup>in vivoin vitrometabolites |
| spellingShingle | Jingjing Tu Qiaoling Li Benhong Zhou The Tannins from <i>Sanguisorba officinalis</i> L. (Rosaceae): A Systematic Study on the Metabolites of Rats Based on HPLC–LTQ–Orbitrap MS<sup>2</sup> Analysis Molecules <i>Sanguisorba</i> tannins HPLC–LTQ–Orbitrap MS<sup>2</sup> in vivo in vitro metabolites |
| title | The Tannins from <i>Sanguisorba officinalis</i> L. (Rosaceae): A Systematic Study on the Metabolites of Rats Based on HPLC–LTQ–Orbitrap MS<sup>2</sup> Analysis |
| title_full | The Tannins from <i>Sanguisorba officinalis</i> L. (Rosaceae): A Systematic Study on the Metabolites of Rats Based on HPLC–LTQ–Orbitrap MS<sup>2</sup> Analysis |
| title_fullStr | The Tannins from <i>Sanguisorba officinalis</i> L. (Rosaceae): A Systematic Study on the Metabolites of Rats Based on HPLC–LTQ–Orbitrap MS<sup>2</sup> Analysis |
| title_full_unstemmed | The Tannins from <i>Sanguisorba officinalis</i> L. (Rosaceae): A Systematic Study on the Metabolites of Rats Based on HPLC–LTQ–Orbitrap MS<sup>2</sup> Analysis |
| title_short | The Tannins from <i>Sanguisorba officinalis</i> L. (Rosaceae): A Systematic Study on the Metabolites of Rats Based on HPLC–LTQ–Orbitrap MS<sup>2</sup> Analysis |
| title_sort | tannins from i sanguisorba officinalis i l rosaceae a systematic study on the metabolites of rats based on hplc ltq orbitrap ms sup 2 sup analysis |
| topic | <i>Sanguisorba</i> tannins HPLC–LTQ–Orbitrap MS<sup>2</sup> in vivo in vitro metabolites |
| url | https://www.mdpi.com/1420-3049/26/13/4053 |
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