The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers
Hair follicle outer root sheath (ORS) is a putative source of stem cells with therapeutic capacity. ORS contains several multipotent stem cell populations, primarily in the distal compartment of the bulge region. However, the bulge is routinely obtained using invasive isolation methods, which requir...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/2/154 |
| _version_ | 1797407721224404992 |
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| author | Hanluo Li Federica Francesca Masieri Marie Schneider Alexander Bartella Sebastian Gaus Sebastian Hahnel Rüdiger Zimmerer Ulrich Sack Danijela Maksimovic-Ivanic Sanja Mijatovic Jan-Christoph Simon Bernd Lethaus Vuk Savkovic |
| author_facet | Hanluo Li Federica Francesca Masieri Marie Schneider Alexander Bartella Sebastian Gaus Sebastian Hahnel Rüdiger Zimmerer Ulrich Sack Danijela Maksimovic-Ivanic Sanja Mijatovic Jan-Christoph Simon Bernd Lethaus Vuk Savkovic |
| author_sort | Hanluo Li |
| collection | DOAJ |
| description | Hair follicle outer root sheath (ORS) is a putative source of stem cells with therapeutic capacity. ORS contains several multipotent stem cell populations, primarily in the distal compartment of the bulge region. However, the bulge is routinely obtained using invasive isolation methods, which require human scalp tissue ex vivo. Non-invasive sampling has been standardized by means of the plucking procedure, enabling to reproducibly obtain the mid-ORS part. The mid-ORS shows potential for giving rise to multiple stem cell populations in vitro. To demonstrate the phenotypic features of distal, middle, and proximal ORS parts, gene and protein expression profiles were studied in physically separated portions. The mid-part of the ORS showed a comparable or higher NGFR, nestin/<i>NES</i>, CD34, <i>CD73</i>, <i>CD44</i>, <i>CD133</i>, <i>CK5</i>, <i>PAX3</i>, <i>MITF</i>, and PMEL expression on both protein and gene levels, when compared to the distal ORS part. Distinct subpopulations of cells exhibiting small and round morphology were characterized with flow cytometry as simultaneously expressing CD73/CD271, CD49f/CD105, nestin, and not CK10. Potentially, these distinct subpopulations can give rise to cultured neuroectodermal and mesenchymal stem cell populations in vitro. In conclusion, the mid part of the ORS holds the potential for yielding multiple stem cells, in particular mesenchymal stem cells. |
| first_indexed | 2024-03-09T03:45:42Z |
| format | Article |
| id | doaj.art-9f7b9c2b83a44c7bb483368496bfa678 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T03:45:42Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-9f7b9c2b83a44c7bb483368496bfa6782023-12-03T14:35:16ZengMDPI AGBiomolecules2218-273X2021-01-0111215410.3390/biom11020154The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell MarkersHanluo Li0Federica Francesca Masieri1Marie Schneider2Alexander Bartella3Sebastian Gaus4Sebastian Hahnel5Rüdiger Zimmerer6Ulrich Sack7Danijela Maksimovic-Ivanic8Sanja Mijatovic9Jan-Christoph Simon10Bernd Lethaus11Vuk Savkovic12Department of Cranial Maxillofacial Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, GermanySchool of (EAST) Engineering, Arts, Science & Technology, University of Suffolk, Ipswich IP 41QJ, UKClinic for Hematology, Cell Therapy and Hemostaseology, University Hospital Leipzig, 04103 Leipzig, GermanyDepartment of Cranial Maxillofacial Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, GermanyDepartment of Cranial Maxillofacial Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, GermanyPolyclinic for Dental Prosthetics and Material Sciences, University Hospital Leipzig, 04103 Leipzig, GermanyDepartment of Cranial Maxillofacial Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, GermanyMedical Faculty, Institute for Clinical Immunology, University Hospital Leipzig, 04103 Leipzig, GermanyDepartment of Immunology, Institute for Biological Research ‘Sinisa Stankovic’ (IBISS)-National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaDepartment of Immunology, Institute for Biological Research ‘Sinisa Stankovic’ (IBISS)-National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaClinic for Dermatology, Venereology and Allergology, University Hospital Leipzig, 04103 Leipzig, GermanyDepartment of Cranial Maxillofacial Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, GermanyDepartment of Cranial Maxillofacial Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, GermanyHair follicle outer root sheath (ORS) is a putative source of stem cells with therapeutic capacity. ORS contains several multipotent stem cell populations, primarily in the distal compartment of the bulge region. However, the bulge is routinely obtained using invasive isolation methods, which require human scalp tissue ex vivo. Non-invasive sampling has been standardized by means of the plucking procedure, enabling to reproducibly obtain the mid-ORS part. The mid-ORS shows potential for giving rise to multiple stem cell populations in vitro. To demonstrate the phenotypic features of distal, middle, and proximal ORS parts, gene and protein expression profiles were studied in physically separated portions. The mid-part of the ORS showed a comparable or higher NGFR, nestin/<i>NES</i>, CD34, <i>CD73</i>, <i>CD44</i>, <i>CD133</i>, <i>CK5</i>, <i>PAX3</i>, <i>MITF</i>, and PMEL expression on both protein and gene levels, when compared to the distal ORS part. Distinct subpopulations of cells exhibiting small and round morphology were characterized with flow cytometry as simultaneously expressing CD73/CD271, CD49f/CD105, nestin, and not CK10. Potentially, these distinct subpopulations can give rise to cultured neuroectodermal and mesenchymal stem cell populations in vitro. In conclusion, the mid part of the ORS holds the potential for yielding multiple stem cells, in particular mesenchymal stem cells.https://www.mdpi.com/2218-273X/11/2/154hair follicleouter root sheathmesenchymal stem cellsneuroectodermal cellsnon-invasive samplingmultipotency |
| spellingShingle | Hanluo Li Federica Francesca Masieri Marie Schneider Alexander Bartella Sebastian Gaus Sebastian Hahnel Rüdiger Zimmerer Ulrich Sack Danijela Maksimovic-Ivanic Sanja Mijatovic Jan-Christoph Simon Bernd Lethaus Vuk Savkovic The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers Biomolecules hair follicle outer root sheath mesenchymal stem cells neuroectodermal cells non-invasive sampling multipotency |
| title | The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers |
| title_full | The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers |
| title_fullStr | The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers |
| title_full_unstemmed | The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers |
| title_short | The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers |
| title_sort | middle part of the plucked hair follicle outer root sheath is identified as an area rich in lineage specific stem cell markers |
| topic | hair follicle outer root sheath mesenchymal stem cells neuroectodermal cells non-invasive sampling multipotency |
| url | https://www.mdpi.com/2218-273X/11/2/154 |
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