The Prevalence of Fluoroquinolone Resistant Genes in Clinical Isolates of Pseudomonas Aeruginosa of Plasmid Origin Isolated From Patients Admitted to Alzahra Hospital of Isfahan
Background: Production of plasmid-dependent quinolone resistant proteins is a major problem in treating infections, but the effect of these proteins on antibiotic resistance has not been explored enough. Therefore, this study was performed to determine the frequency of these genes in clinical isolat...
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2022-04-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | https://jims.mui.ac.ir/article_16327_eb6d15ac49a3b0df1bf687d8bdad96f4.pdf |
| _version_ | 1797704137188573184 |
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| author | Behrooz Ataei Abbasali Javadi Leila Zarghami Fatemeh Nikookar Morteza Pourahmad |
| author_facet | Behrooz Ataei Abbasali Javadi Leila Zarghami Fatemeh Nikookar Morteza Pourahmad |
| author_sort | Behrooz Ataei |
| collection | DOAJ |
| description | Background: Production of plasmid-dependent quinolone resistant proteins is a major problem in treating infections, but the effect of these proteins on antibiotic resistance has not been explored enough. Therefore, this study was performed to determine the frequency of these genes in clinical isolates of Pseudomonas aeruginosa in Al-Zahra Hospital of Isfahan.Methods: This cross-sectional study performed in Alzahra Hospital in Isfahan during 2019-2020. Cultures from 100 samples, positive for Pseudomonas aeruginosa were examined for the presence of qnrA, qnrB and qnrS genes by PCR and the frequency of this gene was assessed based on the ward and the source of the sample.Findings: Out of 100 Pseudomonas aeruginosa samples, 56 (56%) had at least one qnr gene. Thirteen of these samples contained two genes and one contained three qnr females. The frequency of gene based on hospital wards was significantly different (P < 0.001) but the number of genes based on hospital wards was not significantly different (P = 0.57). The frequency of qnrA in all samples was higher than qnrB and qnrS, but the type of gene did not differ significantly in terms of the sampling location (P = 0.36). Also, the frequency of the number of genes observed based on the sample source was not significantly different (P = 0.98).Conclusion: The findings of the present study indicate that the frequency of qnr genes in fluoroquinolone resistant (PMQR) samples in Pseudomodonas aeruginosa is high and this factor can lead to progressive antimicrobial resistance in different hospital wards. Therefore, it seems that the development of suitable antibiotic administration in hospitalized patients is an effective measure in controlling the spread of antibiotic resistance. |
| first_indexed | 2024-03-12T05:15:53Z |
| format | Article |
| id | doaj.art-9f7c02ce380b4706955af0cb71594186 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T05:15:53Z |
| publishDate | 2022-04-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-9f7c02ce380b4706955af0cb715941862023-09-03T08:09:15ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2022-04-014066212412910.48305/jims.v40.i662.012416327The Prevalence of Fluoroquinolone Resistant Genes in Clinical Isolates of Pseudomonas Aeruginosa of Plasmid Origin Isolated From Patients Admitted to Alzahra Hospital of IsfahanBehrooz Ataei0Abbasali Javadi1Leila Zarghami2Fatemeh Nikookar3Morteza Pourahmad4Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Infection Diseases, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranResident of Infectious Diseases, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranResident of Infectious Diseases, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranNosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, IranBackground: Production of plasmid-dependent quinolone resistant proteins is a major problem in treating infections, but the effect of these proteins on antibiotic resistance has not been explored enough. Therefore, this study was performed to determine the frequency of these genes in clinical isolates of Pseudomonas aeruginosa in Al-Zahra Hospital of Isfahan.Methods: This cross-sectional study performed in Alzahra Hospital in Isfahan during 2019-2020. Cultures from 100 samples, positive for Pseudomonas aeruginosa were examined for the presence of qnrA, qnrB and qnrS genes by PCR and the frequency of this gene was assessed based on the ward and the source of the sample.Findings: Out of 100 Pseudomonas aeruginosa samples, 56 (56%) had at least one qnr gene. Thirteen of these samples contained two genes and one contained three qnr females. The frequency of gene based on hospital wards was significantly different (P < 0.001) but the number of genes based on hospital wards was not significantly different (P = 0.57). The frequency of qnrA in all samples was higher than qnrB and qnrS, but the type of gene did not differ significantly in terms of the sampling location (P = 0.36). Also, the frequency of the number of genes observed based on the sample source was not significantly different (P = 0.98).Conclusion: The findings of the present study indicate that the frequency of qnr genes in fluoroquinolone resistant (PMQR) samples in Pseudomodonas aeruginosa is high and this factor can lead to progressive antimicrobial resistance in different hospital wards. Therefore, it seems that the development of suitable antibiotic administration in hospitalized patients is an effective measure in controlling the spread of antibiotic resistance.https://jims.mui.ac.ir/article_16327_eb6d15ac49a3b0df1bf687d8bdad96f4.pdfqnr genefluoroquinoloneantibiotic resistancepseudomonas aeruginosa |
| spellingShingle | Behrooz Ataei Abbasali Javadi Leila Zarghami Fatemeh Nikookar Morteza Pourahmad The Prevalence of Fluoroquinolone Resistant Genes in Clinical Isolates of Pseudomonas Aeruginosa of Plasmid Origin Isolated From Patients Admitted to Alzahra Hospital of Isfahan مجله دانشکده پزشکی اصفهان qnr gene fluoroquinolone antibiotic resistance pseudomonas aeruginosa |
| title | The Prevalence of Fluoroquinolone Resistant Genes in Clinical Isolates of Pseudomonas Aeruginosa of Plasmid Origin Isolated From Patients Admitted to Alzahra Hospital of Isfahan |
| title_full | The Prevalence of Fluoroquinolone Resistant Genes in Clinical Isolates of Pseudomonas Aeruginosa of Plasmid Origin Isolated From Patients Admitted to Alzahra Hospital of Isfahan |
| title_fullStr | The Prevalence of Fluoroquinolone Resistant Genes in Clinical Isolates of Pseudomonas Aeruginosa of Plasmid Origin Isolated From Patients Admitted to Alzahra Hospital of Isfahan |
| title_full_unstemmed | The Prevalence of Fluoroquinolone Resistant Genes in Clinical Isolates of Pseudomonas Aeruginosa of Plasmid Origin Isolated From Patients Admitted to Alzahra Hospital of Isfahan |
| title_short | The Prevalence of Fluoroquinolone Resistant Genes in Clinical Isolates of Pseudomonas Aeruginosa of Plasmid Origin Isolated From Patients Admitted to Alzahra Hospital of Isfahan |
| title_sort | prevalence of fluoroquinolone resistant genes in clinical isolates of pseudomonas aeruginosa of plasmid origin isolated from patients admitted to alzahra hospital of isfahan |
| topic | qnr gene fluoroquinolone antibiotic resistance pseudomonas aeruginosa |
| url | https://jims.mui.ac.ir/article_16327_eb6d15ac49a3b0df1bf687d8bdad96f4.pdf |
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