Genome-wide analysis of the WRKY gene family and its response to abiotic stress in buckwheat (Fagopyrum tataricum)
The WRKY gene family is an ancient plant transcription factor (TF) family with a vital role in plant growth and development, especially in response to biotic and abiotic stresses. Although many researchers have studied WRKY TFs in numerous plant species, little is known of them in Tartary buckwheat...
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| Format: | Article |
| Language: | English |
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De Gruyter
2019-03-01
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| Series: | Open Life Sciences |
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| Online Access: | https://doi.org/10.1515/biol-2019-0010 |
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| author | He Xia Li Jing-jian Chen Yuan Yang Jia-qi Chen Xiao-yang |
| author_facet | He Xia Li Jing-jian Chen Yuan Yang Jia-qi Chen Xiao-yang |
| author_sort | He Xia |
| collection | DOAJ |
| description | The WRKY gene family is an ancient plant transcription factor (TF) family with a vital role in plant growth and development, especially in response to biotic and abiotic stresses. Although many researchers have studied WRKY TFs in numerous plant species, little is known of them in Tartary buckwheat (Fagopyrum tataricum). Based on the recently reported genome sequence of Tartary buckwheat, we identified 78 FtWRKY proteins that could be classified into three major groups. All 77 WRKY genes were distributed unevenly across all eight chromosomes. Exon–intron analysis and motif composition prediction revealed the complexity and diversity of FtWRKYs, indicating that WRKY TFs may be of significance in plant growth regulation and stress response. Two separate pairs of tandem duplication genes were found, but no segmental duplications were identified. Overall, most orthologous gene-pairs between Tartary and common buckwheat evolved under strong purifying selection. qRT-PCR was used to analyze differences in expression among four FtWRKYs (FtWRKY6, 74, 31, and 7) under salt, drought, cold, and heat treatments. The results revealed that all four proteins are related to abiotic stress responses, although they exhibited various expression patterns. In particular, the relative expression levels of FtWRKY6, 74, and 31 were significantly upregulated under salt stress, while the highest expression of FtWRKY7 was observed from heat treatment. This study provides comprehensive insights into the WRKY gene family in Tartary buckwheat, and can support the screening of additional candidate genes for further functional characterization of WRKYs under various stresses. |
| first_indexed | 2024-12-19T12:51:10Z |
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| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-12-19T12:51:10Z |
| publishDate | 2019-03-01 |
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| series | Open Life Sciences |
| spelling | doaj.art-9f804e6d239948d1bc8bd4069c4f7bd32022-12-21T20:20:33ZengDe GruyterOpen Life Sciences2391-54122019-03-01141809610.1515/biol-2019-0010biol-2019-0010Genome-wide analysis of the WRKY gene family and its response to abiotic stress in buckwheat (Fagopyrum tataricum)He Xia0Li Jing-jian1Chen Yuan2Yang Jia-qi3Chen Xiao-yang4State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources (South China Agricultural University), Guangzhou510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources (South China Agricultural University), Guangzhou510642, ChinaGuangdong Province Research Center of Woody Forage Engineering Technology, Guangzhou510642, ChinaGuangdong Province Research Center of Woody Forage Engineering Technology, Guangzhou510642, Chinaushan road NO.483 Guangzhou city, GuangdongGuangzhou, P.R.ChinaThe WRKY gene family is an ancient plant transcription factor (TF) family with a vital role in plant growth and development, especially in response to biotic and abiotic stresses. Although many researchers have studied WRKY TFs in numerous plant species, little is known of them in Tartary buckwheat (Fagopyrum tataricum). Based on the recently reported genome sequence of Tartary buckwheat, we identified 78 FtWRKY proteins that could be classified into three major groups. All 77 WRKY genes were distributed unevenly across all eight chromosomes. Exon–intron analysis and motif composition prediction revealed the complexity and diversity of FtWRKYs, indicating that WRKY TFs may be of significance in plant growth regulation and stress response. Two separate pairs of tandem duplication genes were found, but no segmental duplications were identified. Overall, most orthologous gene-pairs between Tartary and common buckwheat evolved under strong purifying selection. qRT-PCR was used to analyze differences in expression among four FtWRKYs (FtWRKY6, 74, 31, and 7) under salt, drought, cold, and heat treatments. The results revealed that all four proteins are related to abiotic stress responses, although they exhibited various expression patterns. In particular, the relative expression levels of FtWRKY6, 74, and 31 were significantly upregulated under salt stress, while the highest expression of FtWRKY7 was observed from heat treatment. This study provides comprehensive insights into the WRKY gene family in Tartary buckwheat, and can support the screening of additional candidate genes for further functional characterization of WRKYs under various stresses.https://doi.org/10.1515/biol-2019-0010fagopyrum tataricumwrky gene familybioinformaticgenome-wide analysisabiotic stress |
| spellingShingle | He Xia Li Jing-jian Chen Yuan Yang Jia-qi Chen Xiao-yang Genome-wide analysis of the WRKY gene family and its response to abiotic stress in buckwheat (Fagopyrum tataricum) Open Life Sciences fagopyrum tataricum wrky gene family bioinformatic genome-wide analysis abiotic stress |
| title | Genome-wide analysis of the WRKY gene family and its response to abiotic stress in buckwheat (Fagopyrum tataricum) |
| title_full | Genome-wide analysis of the WRKY gene family and its response to abiotic stress in buckwheat (Fagopyrum tataricum) |
| title_fullStr | Genome-wide analysis of the WRKY gene family and its response to abiotic stress in buckwheat (Fagopyrum tataricum) |
| title_full_unstemmed | Genome-wide analysis of the WRKY gene family and its response to abiotic stress in buckwheat (Fagopyrum tataricum) |
| title_short | Genome-wide analysis of the WRKY gene family and its response to abiotic stress in buckwheat (Fagopyrum tataricum) |
| title_sort | genome wide analysis of the wrky gene family and its response to abiotic stress in buckwheat fagopyrum tataricum |
| topic | fagopyrum tataricum wrky gene family bioinformatic genome-wide analysis abiotic stress |
| url | https://doi.org/10.1515/biol-2019-0010 |
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