The parabasal filaments of Trichomonas vaginalis: A new filament and observations using 0.8 nm-resolution scanning electron microscopy
Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common nonviral sexually transmitted infection worldwide, with an estimated 260 million new cases annually. T. vaginalis contains organelles common to all eukaryotic cells, uncommon cell structures such as hydrogenosomes, and a...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Journal of Structural Biology: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590152424000047 |
| _version_ | 1827222984437268480 |
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| author | Sharmila Fiama das Neves Ortiz Raphael Verdan Gustavo Miranda Rocha Kildare Miranda Marlene Benchimol |
| author_facet | Sharmila Fiama das Neves Ortiz Raphael Verdan Gustavo Miranda Rocha Kildare Miranda Marlene Benchimol |
| author_sort | Sharmila Fiama das Neves Ortiz |
| collection | DOAJ |
| description | Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common nonviral sexually transmitted infection worldwide, with an estimated 260 million new cases annually. T. vaginalis contains organelles common to all eukaryotic cells, uncommon cell structures such as hydrogenosomes, and a complex and elaborate cytoskeleton constituting the mastigont system. The mastigont system is mainly formed by several proteinaceous structures associated with basal bodies, the pelta-axostylar complex made of microtubules, and striated filaments named the costa and the parabasal filaments (PFs). Although the structural organization of trichomonad cytoskeletons has been analyzed using several techniques, observation using a new generation of scanning electron microscopes with a resolution exceeding 1 nm has allowed more detailed visualization of the three-dimensional organization of the mastigont system. In this study, we have investigated the cytoskeleton of T. vaginalis using a diverse range of scanning probe microscopy techniques, which were complemented by electron tomography and Fast-Fourier methods. This multi-modal approach has allowed us to characterize an unknown parabasal filament and reveal the ultrastructure of other striated fibers that have not been published before. Here, we show the differences in origin, striation pattern, size, localization, and additional details of the PFs, thus improving the knowledge of the cell biology of this parasite. |
| first_indexed | 2024-04-25T01:41:16Z |
| format | Article |
| id | doaj.art-9f80f5090f5c4ec0ab4a992b7262ef4f |
| institution | Directory Open Access Journal |
| issn | 2590-1524 |
| language | English |
| last_indexed | 2025-03-21T16:47:10Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Structural Biology: X |
| spelling | doaj.art-9f80f5090f5c4ec0ab4a992b7262ef4f2024-06-16T05:46:31ZengElsevierJournal of Structural Biology: X2590-15242024-06-019100099The parabasal filaments of Trichomonas vaginalis: A new filament and observations using 0.8 nm-resolution scanning electron microscopySharmila Fiama das Neves Ortiz0Raphael Verdan1Gustavo Miranda Rocha2Kildare Miranda3Marlene Benchimol4Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilUnidade de Pesquisa Urogenital, Centro Biomédico, Departamento de Anatomia, Universidade Estadual do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; CENABIO - Centro Nacional de Biologia Estrutural e Bioimagem, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil; Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagem – Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; Centro Multiusuário para Análise de Fenômenos Biomédicos, Universidade do Estado do Amazonas, Manaus, BrazilCENABIO - Centro Nacional de Biologia Estrutural e Bioimagem, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil; Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagem – Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; UNIGRANRIO-Universidade da Grande Rio, Rio de Janeiro, Brazil; Corresponding author at: CENABIO - Centro Nacional de Biologia Estrutural e Bioimagem, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common nonviral sexually transmitted infection worldwide, with an estimated 260 million new cases annually. T. vaginalis contains organelles common to all eukaryotic cells, uncommon cell structures such as hydrogenosomes, and a complex and elaborate cytoskeleton constituting the mastigont system. The mastigont system is mainly formed by several proteinaceous structures associated with basal bodies, the pelta-axostylar complex made of microtubules, and striated filaments named the costa and the parabasal filaments (PFs). Although the structural organization of trichomonad cytoskeletons has been analyzed using several techniques, observation using a new generation of scanning electron microscopes with a resolution exceeding 1 nm has allowed more detailed visualization of the three-dimensional organization of the mastigont system. In this study, we have investigated the cytoskeleton of T. vaginalis using a diverse range of scanning probe microscopy techniques, which were complemented by electron tomography and Fast-Fourier methods. This multi-modal approach has allowed us to characterize an unknown parabasal filament and reveal the ultrastructure of other striated fibers that have not been published before. Here, we show the differences in origin, striation pattern, size, localization, and additional details of the PFs, thus improving the knowledge of the cell biology of this parasite.http://www.sciencedirect.com/science/article/pii/S2590152424000047CytoskeletonTrichomonadsNew filamentStriated fibersFast Fourier transformTomography |
| spellingShingle | Sharmila Fiama das Neves Ortiz Raphael Verdan Gustavo Miranda Rocha Kildare Miranda Marlene Benchimol The parabasal filaments of Trichomonas vaginalis: A new filament and observations using 0.8 nm-resolution scanning electron microscopy Journal of Structural Biology: X Cytoskeleton Trichomonads New filament Striated fibers Fast Fourier transform Tomography |
| title | The parabasal filaments of Trichomonas vaginalis: A new filament and observations using 0.8 nm-resolution scanning electron microscopy |
| title_full | The parabasal filaments of Trichomonas vaginalis: A new filament and observations using 0.8 nm-resolution scanning electron microscopy |
| title_fullStr | The parabasal filaments of Trichomonas vaginalis: A new filament and observations using 0.8 nm-resolution scanning electron microscopy |
| title_full_unstemmed | The parabasal filaments of Trichomonas vaginalis: A new filament and observations using 0.8 nm-resolution scanning electron microscopy |
| title_short | The parabasal filaments of Trichomonas vaginalis: A new filament and observations using 0.8 nm-resolution scanning electron microscopy |
| title_sort | parabasal filaments of trichomonas vaginalis a new filament and observations using 0 8 nm resolution scanning electron microscopy |
| topic | Cytoskeleton Trichomonads New filament Striated fibers Fast Fourier transform Tomography |
| url | http://www.sciencedirect.com/science/article/pii/S2590152424000047 |
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