Mechanistic insights into inositol-mediated rumen function promotion and metabolic alteration using in vitro and in vivo models
Inositol is a bioactive factor that is widely found in nature; however, there are few studies on its use in ruminant nutrition. This study investigated the effects of different inositol doses and fermentation times on rumen fermentation and microbial diversity, as well as the levels of rumen and blo...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1359234/full |
| _version_ | 1797305310696701952 |
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| author | Guopei Yin Zhe Sun Zhe Sun Zhe Sun Zhanqing Wang Yuanhong Xia Long Cheng Guixin Qin Natnael D. Aschalew Natnael D. Aschalew Hongyun Liu Xuefeng Zhang Qilu Wu Weigang Zhang Wei Zhao Tao Wang Tao Wang Yuguo Zhen Yuguo Zhen |
| author_facet | Guopei Yin Zhe Sun Zhe Sun Zhe Sun Zhanqing Wang Yuanhong Xia Long Cheng Guixin Qin Natnael D. Aschalew Natnael D. Aschalew Hongyun Liu Xuefeng Zhang Qilu Wu Weigang Zhang Wei Zhao Tao Wang Tao Wang Yuguo Zhen Yuguo Zhen |
| author_sort | Guopei Yin |
| collection | DOAJ |
| description | Inositol is a bioactive factor that is widely found in nature; however, there are few studies on its use in ruminant nutrition. This study investigated the effects of different inositol doses and fermentation times on rumen fermentation and microbial diversity, as well as the levels of rumen and blood metabolites in sheep. Rumen fermentation parameters, microbial diversity, and metabolites after different inositol doses were determined in vitro. According to the in vitro results, six small-tailed Han sheep fitted with permanent rumen fistulas were used in a 3 × 3 Latin square feeding experiment where inositol was injected into the rumen twice a day and rumen fluid and blood samples were collected. The in vitro results showed that inositol could increase in vitro dry matter digestibility, in vitro crude protein digestibility, NH3-N, acetic acid, propionic acid, and rumen microbial diversity and affect rumen metabolic pathways (p < 0.05). The feeding experiment results showed that inositol increased the blood concentration of high-density lipoprotein and IgG, IgM, and IL-4 levels. The rumen microbial composition was significantly affected (p < 0.05). Differential metabolites in the rumen were mainly involved in ABC transporters, biotin metabolism, and phenylalanine metabolism, whereas those in the blood were mainly involved in arginine biosynthesis and glutathione and tyrosine metabolism. In conclusion, inositol improves rumen function, affects rumen microorganisms and rumen and blood metabolites and may reduce inflammation, improving animal health. |
| first_indexed | 2024-03-08T00:24:04Z |
| format | Article |
| id | doaj.art-9f8a8576e6a142c2946794df8f1fafd3 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-08T00:24:04Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-9f8a8576e6a142c2946794df8f1fafd32024-02-16T04:25:41ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-02-011110.3389/fvets.2024.13592341359234Mechanistic insights into inositol-mediated rumen function promotion and metabolic alteration using in vitro and in vivo modelsGuopei Yin0Zhe Sun1Zhe Sun2Zhe Sun3Zhanqing Wang4Yuanhong Xia5Long Cheng6Guixin Qin7Natnael D. Aschalew8Natnael D. Aschalew9Hongyun Liu10Xuefeng Zhang11Qilu Wu12Weigang Zhang13Wei Zhao14Tao Wang15Tao Wang16Yuguo Zhen17Yuguo Zhen18Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, Key Laboratory of Animal Production Product Quality and Security Ministry of Education, JLAU-Borui Dairy Science and Technology R&D Center, College of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Nutrition and Feed Science of Jilin Province, Key Laboratory of Animal Production Product Quality and Security Ministry of Education, JLAU-Borui Dairy Science and Technology R&D Center, College of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaCollege of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun, ChinaPostdoctoral Scientific Research Workstation, Feed Engineering Technology Research Center of Jilin Province, Changchun Borui Science and Technology Co., Ltd., Changchun, ChinaKey Laboratory of Animal Nutrition and Feed Science of Jilin Province, Key Laboratory of Animal Production Product Quality and Security Ministry of Education, JLAU-Borui Dairy Science and Technology R&D Center, College of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Nutrition and Feed Science of Jilin Province, Key Laboratory of Animal Production Product Quality and Security Ministry of Education, JLAU-Borui Dairy Science and Technology R&D Center, College of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Nutrition and Feed Science of Jilin Province, Key Laboratory of Animal Production Product Quality and Security Ministry of Education, JLAU-Borui Dairy Science and Technology R&D Center, College of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Nutrition and Feed Science of Jilin Province, Key Laboratory of Animal Production Product Quality and Security Ministry of Education, JLAU-Borui Dairy Science and Technology R&D Center, College of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Nutrition and Feed Science of Jilin Province, Key Laboratory of Animal Production Product Quality and Security Ministry of Education, JLAU-Borui Dairy Science and Technology R&D Center, College of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaCollege of Agriculture and Environmental Science, Dilla University, Dila, EthiopiaCollege of Animal Sciences, Zhejiang University, Hangzhou, ChinaKey Laboratory of Animal Nutrition and Feed Science of Jilin Province, Key Laboratory of Animal Production Product Quality and Security Ministry of Education, JLAU-Borui Dairy Science and Technology R&D Center, College of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaCollege of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun, ChinaPostdoctoral Scientific Research Workstation, Feed Engineering Technology Research Center of Jilin Province, Changchun Borui Science and Technology Co., Ltd., Changchun, ChinaPostdoctoral Scientific Research Workstation, Feed Engineering Technology Research Center of Jilin Province, Changchun Borui Science and Technology Co., Ltd., Changchun, ChinaKey Laboratory of Animal Nutrition and Feed Science of Jilin Province, Key Laboratory of Animal Production Product Quality and Security Ministry of Education, JLAU-Borui Dairy Science and Technology R&D Center, College of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaPostdoctoral Scientific Research Workstation, Feed Engineering Technology Research Center of Jilin Province, Changchun Borui Science and Technology Co., Ltd., Changchun, ChinaKey Laboratory of Animal Nutrition and Feed Science of Jilin Province, Key Laboratory of Animal Production Product Quality and Security Ministry of Education, JLAU-Borui Dairy Science and Technology R&D Center, College of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaPostdoctoral Scientific Research Workstation, Feed Engineering Technology Research Center of Jilin Province, Changchun Borui Science and Technology Co., Ltd., Changchun, ChinaInositol is a bioactive factor that is widely found in nature; however, there are few studies on its use in ruminant nutrition. This study investigated the effects of different inositol doses and fermentation times on rumen fermentation and microbial diversity, as well as the levels of rumen and blood metabolites in sheep. Rumen fermentation parameters, microbial diversity, and metabolites after different inositol doses were determined in vitro. According to the in vitro results, six small-tailed Han sheep fitted with permanent rumen fistulas were used in a 3 × 3 Latin square feeding experiment where inositol was injected into the rumen twice a day and rumen fluid and blood samples were collected. The in vitro results showed that inositol could increase in vitro dry matter digestibility, in vitro crude protein digestibility, NH3-N, acetic acid, propionic acid, and rumen microbial diversity and affect rumen metabolic pathways (p < 0.05). The feeding experiment results showed that inositol increased the blood concentration of high-density lipoprotein and IgG, IgM, and IL-4 levels. The rumen microbial composition was significantly affected (p < 0.05). Differential metabolites in the rumen were mainly involved in ABC transporters, biotin metabolism, and phenylalanine metabolism, whereas those in the blood were mainly involved in arginine biosynthesis and glutathione and tyrosine metabolism. In conclusion, inositol improves rumen function, affects rumen microorganisms and rumen and blood metabolites and may reduce inflammation, improving animal health.https://www.frontiersin.org/articles/10.3389/fvets.2024.1359234/fullinositolsheeprumen fermentationrumen microorganismsmetabolomics |
| spellingShingle | Guopei Yin Zhe Sun Zhe Sun Zhe Sun Zhanqing Wang Yuanhong Xia Long Cheng Guixin Qin Natnael D. Aschalew Natnael D. Aschalew Hongyun Liu Xuefeng Zhang Qilu Wu Weigang Zhang Wei Zhao Tao Wang Tao Wang Yuguo Zhen Yuguo Zhen Mechanistic insights into inositol-mediated rumen function promotion and metabolic alteration using in vitro and in vivo models Frontiers in Veterinary Science inositol sheep rumen fermentation rumen microorganisms metabolomics |
| title | Mechanistic insights into inositol-mediated rumen function promotion and metabolic alteration using in vitro and in vivo models |
| title_full | Mechanistic insights into inositol-mediated rumen function promotion and metabolic alteration using in vitro and in vivo models |
| title_fullStr | Mechanistic insights into inositol-mediated rumen function promotion and metabolic alteration using in vitro and in vivo models |
| title_full_unstemmed | Mechanistic insights into inositol-mediated rumen function promotion and metabolic alteration using in vitro and in vivo models |
| title_short | Mechanistic insights into inositol-mediated rumen function promotion and metabolic alteration using in vitro and in vivo models |
| title_sort | mechanistic insights into inositol mediated rumen function promotion and metabolic alteration using in vitro and in vivo models |
| topic | inositol sheep rumen fermentation rumen microorganisms metabolomics |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1359234/full |
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